6*His Monoclonal Antibody
Datasheet-
货号:CSB-MA000011M0m
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规格:¥600
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图片:
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Western Blot
Positive WB detected in: HIS fusion protein 1 at 20ng, 10ng, 5ng, 2.5ng
All lanes: HIS antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
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Western Blot
Positive WB detected in: HIS fusion protein 2 at 20ng, 10ng, 5ng, 2.5ng
All lanes: HIS antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
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其他:
产品详情
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产品描述:6*His monoclonal antibody CSB-MA000011M0m was produced in mouse immunized by using HHHHHH(6x His) synthetic peptide conjugate to KLH as the immunogen. The immunogen, 6x His, a short peptide, consists of six histidine residues (His-His-His-His-His-His). It is specifically designed for the adsorptive purification of recombinant proteins. The relatively small size of His tags makes its integration into expression vectors extremely easy.
This 6*His Monoclonal Antibody was tested in the WB, IHC, ICC and ELISA applications. The non-conjugated IgG2a got purified by protein G and reached up to 95% in purity. It can be used for detection and expression of His-tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of His fusion-expressed proteins and the like. It doesn’t have species restricted. -
别名:6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
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宿主:Mouse
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反应种属:N/A
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免疫原:HHHHHH(6x His) synthetic peptide conjugate to KLH
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标记方式:Non-conjugated
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克隆类型:Monoclonal
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抗体亚型:IgG2a
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纯化方式:>95%, Protein A purified
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克隆号:3G5H8
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浓度:It differs from different batches. Please contact us to confirm it.
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保存缓冲液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
产品提供形式:Liquid
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应用范围:ELISA, WB
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推荐稀释比:
Application Recommended Dilution WB 1:500-1:2000 -
Protocols:
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储存条件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
引用文献
- Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain Dominguez G.et al,Acta Neuropathol Commun,2018