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    Recombinant Mouse Breast cancer type 1 susceptibility protein homolog(Brca1) ,partial

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    • 共轭:
      Avi-tag Biotinylated

      E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

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    • 来源:
      Mammalian cell
    • 其他:


    • 纯度:
      >85% (SDS-PAGE)
    • 基因名:
    • Uniprot No.:
    • 别名:
      Brca1Breast cancer type 1 susceptibility protein homolog; EC; RING-type E3 ubiquitin transferase BRCA1
    • 种属:
      Mus musculus (Mouse)
    • 蛋白长度:
    • 蛋白标签:
      The following tags are available.
      N-terminal His-tagged
      The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
    • 产品提供形式:
      Lyophilized powder
      Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
    • 复溶:
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
    • 储存条件:
      Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
    • 保质期:
      The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
      Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
    • 货期:
      Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
      Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
    • 注意事项:
      Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
    • Datasheet :
      Please contact us to get it.



    • 功能:
      E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Required for FANCD2 targeting to sites of DNA damage. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator.
    • 基因功能参考文献:
      1. Enhanced BRCA1 after cerebral ischemia/reperfusion njury may attenuate or prevent neural damage from ischemia/reperfusion via NRF2-mediated antioxidant pathway. PMID: 30014904
      2. BRCA1 Interacting Protein COBRA1 Facilitates Adaptation to Castrate-Resistant Growth Conditions. PMID: 30036938
      3. Recent work in a TP53(-/-)BRCA1-mutant murine breast cancer model indicates that double blockade with two immune checkpoint inhibitors increases the number of tumor-infiltrating lymphocytes and overall survival after DNA damaging chemotherapy, whereas single blockade does not PMID: 29120748
      4. Data indicate the importance of breast cancer 1 protein (BRCA1)/breast cancer 2 protein (BRCA2) function in cranial neural crest cells (CNCCs) during craniofacial skeletal formation. PMID: 29718910
      5. BRCA1, but not BRCA2, suppresses the formation of tandem duplications at a site-specific chromosomal replication fork barrier imposed by the binding of Tus proteins to an array of Ter sites. BRCA1 has no equivalent role at chromosomal double-stranded DNA breaks, indicating that tandem duplications form specifically at stalled forks. PMID: 29168504
      6. Loss of p16INK4 protein (p16) transforms breast cancer 1 (Brca1)-deficient mammary epithelial cell (MEC) and induces mammary tumors. PMID: 27811360
      7. Brca1 is necessary for hematopoietic stem cells maintenance and normal hematopoiesis and that distinct mutations lead to different degrees of hematopoietic dysfunction. PMID: 28122244
      8. TGFbeta stabilized the abundance of BRCA1 by reducing the abundance of microRNA-182 (miR-182). Ectopic expression of BRCA1 or antagonism of miR-182 in cultured TGFbeta-deficient mammary epithelial cells restored luminal lineage commitment. PMID: 27923913
      9. BRCA1 inactivation can increase expression of miR-155, contributing to cardiac hypertrophy. And Rev produces their beneficial effects partially by down-regulating miR-155 expression, which might be a novel strategy for treatment of cardiac hypertrophy. PMID: 27107135
      10. both murine Brca1185stop tumors and human BRCA1185delAG breast cancer cells express a new gene domain-less (RING-less) BRCA1 protein that mediated resistance to homologous recombination deficient-targeted therapies PMID: 27454287
      11. Genomic instability can be rescued by deletion of Trp53bp1, encoding the DNA damage response factor 53BP1; mice expressing RING-less BRCA1 do not show an increased susceptibility to tumors in the absence of 53BP1; Genomic instability in cells expressing RING-less BRCA1 correlates with loss of BARD1 and a defect in restart of replication forks after hydroxyurea treatment PMID: 27670884
      12. the aberrant proliferative capacity of Brca1(-/-) luminal progenitor cells is linked to the replication-associated DNA damage response, where proliferation of mammary progenitors is perpetuated by damage-induced, autologous NF-kappaB signaling. PMID: 27292187
      13. We report here elevated recombination rates at telomeres in cells from human BRCA1 mutation carriers and in mouse embryonic stem cells lacking both copies of functional Brca1. PMID: 27295426
      14. loss of Brca1, a tumor suppressor that functions in DNA damage repair, in the mammary epithelium induced senescence with induction of p16 and a decline of stem cell function, which was rescued by p16 loss. PMID: 28278054
      15. Brca1-Wwox interaction supports non-homologous end-joining as the dominant DSB repair pathway in Wwox-sufficient cells PMID: 27869163
      16. Structure-guided separation-of-function mutations show that the RNF8 E2 stimulating activity is essential for DSB signaling in mammalian cells and is necessary for downstream recruitment of 53BP1 and BRCA1. PMID: 26903517
      17. Results reveal a broader biological role for BRCA1 in protecting the developing embryo from oxidative stress, and corroborate a role for DNA damage in the mechanism of ethanol embryotoxicity. PMID: 26629949
      18. This study uncovers a previously unappreciated, DNA repair-independent function of BRCA1 in antagonizing COBRA1-dependent transcription program during mammary gland development. PMID: 26941120
      19. loss of Brca1 in murine BM causes hematopoietic defects similar to those seen in people with FA, which provides strong evidence that Brca1 is critical for normal hematopoiesis and that Brca1 is a bona fide FA-like gene. PMID: 26644450
      20. The Brca1 pathway is dysregulated in spinal cord microglia from hSOD1G93A mice. Brca1 was upregulated at 90 days of age when microgliosis became evident. PMID: 26227626
      21. Brca1(GC-/-) models reveal that specific intra-follicular Brca1 loss alone, or combined with cancer-promoting genetic (Trp53 loss) and endocrine (high serum follicle-stimulating hormone) changes, was not sufficient to cause ovarian tumors. PMID: 25943777
      22. Data show that mammary tumors resembling BRCA1-associated metaplastic breast carcinoma display intrinsic resistance to the poly(ADP-ribose) polymerase (PARP) inhibitor olaparib as a result of increased P-glycoprotein drug efflux transporter expression. PMID: 26100884
      23. Downregulation of BRCA1 protein levels was a result of endoplasmic reticulum stress. PMID: 25483082
      24. BRCA1 regulated cell cycle checkpoints in a manner dependent on the 26S proteasome activity. PMID: 25522274
      25. work therefore ascribes novel roles for BRCA1 and CtIP in end-processing and fusion reactions at uncapped telomeres PMID: 25582120
      26. results underscore the in vivo importance of the PALB2-BRCA1 complex formation in DSB repair and male meiosis PMID: 25016020
      27. Data indicate that loss of the CtIP-BRCA1 interaction does not detectably affect resection, maintenance of genomic stability or viability. PMID: 24842372
      28. hypothesis that aberrant long-tract homologous recombination at stalled replication forks contributes to genomic instability and breast/ovarian cancer predisposition in BRCA mutant cells PMID: 24776801
      29. the major meiotic role of BRCA1 is to promote the dramatic chromatin changes required for formation and function of the XY body PMID: 24914237
      30. work elucidates the basis of the tissue specificity of BRCA1-related tumor predisposition, and explains why oophorectomy significantly reduces breast cancer risk and recurrence in women carrying BRCA1 mutations PMID: 24567396
      31. These results suggest distinct apoptotic and centrosomal functions of BRCA1 in neural progenitors. PMID: 24639535
      32. Progesterone receptor A stability is mediated by glycogen synthase kinase-3beta in the Brca1-deficient mammary gland. PMID: 23880761
      33. Cardiomyocyte-specific loss of BRCA1 alters critical pathways of fatty acid and glucose metabolism, leading to an energy starved heart. PMID: 23317938
      34. over-expression of BRCA1 in bone marrow disrupted hematopoiesis through the down-regulation of p21/cip1 and p57. PMID: 23850973
      35. These data collectively highlight a previously unrecognized role of BRCA1 as a gatekeeper of inflammation-induced endothelial cell function and a target to limit atherosclerosis. PMID: 23415688
      36. the role of BRCA1 in regulating Nrf2 activity suggests important implications for both the etiology and treatment of BRCA1-related cancers. PMID: 23857982
      37. BRCA1 is a key negative modulator of PRC2 and that loss of BRCA1 inhibits embryonic stem (ES) cell differentiation and enhances an aggressive breast cancer phenotype by affecting PRC2 function. PMID: 23624935
      38. The phospho-dependent BRCA1-CtIP interaction is not essential for HDR-mediated DSB repair or for tumor suppression. PMID: 23712259
      39. this study demonstrates that germline mutation of Brca1 is not efficient in initiating mammary tumor formation and that loss of p18 results in the expansion of luminal progenitors, which is an early and initiatory factor in luminal tumor development. PMID: 22777348
      40. Impairment of BRCA1-related DNA double-strand break repair leads to ovarian aging in mice and humans. PMID: 23408054
      41. TNRC9 and BRCA1 expression are inversely correlated in breast cancer. TNRC9 binds to CREB and BRCA1 promoter and downregulates BRCA1 promoter activity. PMID: 23447579
      42. Our results indicate that Brca1 deficiency might activate Akt1 contributing to tumorigenesis through regulation of the Chk1-Rad51 signaling. PMID: 22665067
      43. Double-strand break repair by homologous recombination in primary mouse somatic cells requires BRCA1 but not the ATM kinase. PMID: 23509290
      44. reveal a major difference in the requirement of BRCA1 between different types of epidermal stem cells and progenitors and during the different activation stages of adult hair follicle stem cells PMID: 23271346
      45. BrCa1 might help to control fatty acid biosynthesis in adipocytes and adipose tissue from obese subjects PMID: 22666314
      46. BRCA1 directly modulates the spatiotemporal dynamics of gamma-H2AX upon genotoxic stress and serves as a molecular maker for neuronal DNA damage response in Huntington's disease. PMID: 22580959
      47. Mechanistically, loss of RAP80 suppresses recruitment of the BRCA1-A complex to DNA damage sites and abrogates the DNA damage repair process at DNA damage sites. PMID: 22539352
      48. Authors searched for gene expression patterns that correlate with docetaxel or cisplatin response in a mouse model for breast cancer associated with BRCA1 deficiency. PMID: 22396490
      49. Estrogen biosynthesis by granulosa cells was increased in mice carrying a homozygous Brca1 mutation. PMID: 22488153
      50. BRCA1 has an additional role in DNA crosslink repair that is distinct from homologous recombination. PMID: 22445484



    • 亚细胞定位:
      Nucleus. Chromosome. Cytoplasm.
    • 组织特异性:
      In the embryo, expressed in otic vesicles at day 9.5. At day 10.5, this expression decreases and high levels are found in the neuroectoderm. At days 11-12.5, high levels in differentiating keratinocytes and whisker pad primordia. At days 14-17, expression
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