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    Recombinant Human Macrophage colony-stimulating factor 1 receptor(CSF1R),partial

    In Stock
    • 货号:
      CSB-MP006044HUi10
    • 规格:
    • 图片:
      • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • 其他:

    产品详情

    • 纯度:
      Greater than 90% as determined by SDS-PAGE.
    • 基因名:
      CSF1R
    • Uniprot No.:
    • 别名:
      (CSF-1 receptor)(CSF-1-R)(CSF-1R)(M-CSF-R)(Proto-oncogene c-Fms)(CD antigen CD115)
    • 种属:
      Homo sapiens (Human)
    • 蛋白长度:
      Partial
    • 来源:
      Mammalian cell
    • 分子量:
      57.7 kDa
    • 表达区域:
      20-512aa
    • 氨基酸序列
      IPVIEPSVPELVVKPGATVTLRCVGNGSVEWDGPPSPHWTLYSDGSSSILSTNNATFQNTGTYRCTEPGDPLGGSAAIHLYVKDPARPWNVLAQEVVVFEDQDALLPCLLTDPVLEAGVSLVRVRGRPLMRHTNYSFSPWHGFTIHRAKFIQSQDYQCSALMGGRKVMSISIRLKVQKVIPGPPALTLVPAELVRIRGEAAQIVCSASSVDVNFDVFLQHNNTKLAIPQQSDFHNNRYQKVLTLNLDQVDFQHAGNYSCVASNVQGKHSTSMFFRVVESAYLNLSSEQNLIQEVTVGEGLNLKVMVEAYPGLQGFNWTYLGPFSDHQPEPKLANATTKDTYRHTFTLSLPRLKPSEAGRYSFLARNPGGWRALTFELTLRYPPEVSVIWTFINGSGTLLCAASGYPQPNVTWLQCSGHTDRCDEAQVLQVWDDPYPEVLSQEPFHKVTVQSLLTVETLEHNQTYECRAHNSVGSGSWAFIPISAGAHTHPPDE
      Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
    • 蛋白标签:
      C-terminal 6xHis-Flag-tagged
    • 产品提供形式:
      Liquid or Lyophilized powder
      Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
    • 缓冲液:
      If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
    • 复溶:
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
    • 储存条件:
      Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
    • 保质期:
      The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
      Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
    • 货期:
      3-7 business days
    • 注意事项:
      Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
    • Datasheet & COA:
      Please contact us to get it.

    产品评价

    靶点详情

    • 功能:
      Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages.
    • 基因功能参考文献:
      1. M-CSFR inhibition suppressed programmed death-1 and -2 ligand in adult T-cell leukemia/lymphoma (ATLL) cells and macrophages stimulated with conditioned medium from ATL-T cells. PMID: 30541986
      2. The detection of the CSF1R mutation outside of the region-encoding TKD may extend the genetic spectrum of adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP) with CSF1R mutations. Mutational analysis of all the coding exons of CSF1R should be considered for patients clinically suspected of having ALSP. PMID: 30136118
      3. To verify its sensitivity and specificity, we retrospectively applied our criteria to 83 axonal spheroids and pigmented glia cases who had CSF1R mutations PMID: 28921817
      4. Study find elevated expression of CSF1R in primary gastric cancer tissue (GC) to be significantly associated with the presence of lymph node and peritoneal metastasis, advanced TNM stage, and poor survival. In vitro analysis also revealed a functional role for the CSF1R in GC development, and a prognostic and predictive biomarker for GC. PMID: 29767252
      5. Adult-onset Mendelian leukodystrophy genes are not common factors implicated in Alzheimer's disease, but there is a potential pathogenic link between NOTCH3, CSF1R, and sporadic late-onset Alzheimer's disease. PMID: 29544907
      6. Adult-onset leukoencephalopathy with axonal spheroids and pigmented glia.(S39-S41) is a subtype of dominantly inherited leukoencephalopathy caused by CSF1R mutations. PMID: 28827005
      7. this is the first study to demonstrate CSF1R genetic variant regulates the CSF-1R signaling and sensitivity to CSF-1R inhibitors. PMID: 28724665
      8. Hypoxia promotes glioma-associated macrophage infiltration via periostin and subsequent M2 polarization by upregulating TGF-beta and M-CSFR. PMID: 27602954
      9. CSF-1R is a novel therapeutic target. PMID: 27334834
      10. The phenotype of adult-onset leukoencephalopathy axonal spheroids and pigmented glia caused by CSF1R mutations is affected by sex PMID: 27680516
      11. CSFIR mutation is associated with Metaplastic Breast Cancer. PMID: 27568101
      12. Results suggest that TP63 rs7631358 G > A and CSF1R rs10079250 A > G may affect the risk and prognosis of lung cancer in never-smoking females. PMID: 28449811
      13. findings suggest that expression of wild-type CSF1R in some cells, whether achieved by mosaicism or chimerism, may confer benefit in hereditary diffuse leukoencephalopathy with axonal spheroids. PMID: 27190017
      14. This review showed that CSF1R mutation is related to Hereditary diffuse leukoencephalopathy with axonal spheroids. PMID: 27338940
      15. High CSF-1R expression is associated with Clear Cell Renal Cell Carcinoma. PMID: 26467457
      16. The aim of this study was to compare the expression of CSF-1R in nasopharyngeal carcinoma to nasopharyngitis. PMID: 26743272
      17. CSF1R mutations account for 10% of idiopathic adult onset leukodystrophies. PMID: 25935893
      18. The frequencies of the rare alleles of CCR2, ITGB3, and 3'UTR of c-fms in the Old Believers are lower than in the sample of Novosibirsk Russians, and the rare allele of DBH is more frequent PMID: 27239844
      19. Assessing serum levels of WFA(+) -CSF1R has diagnostic value for predicting carcinogenesis and the survival of LC patients. PMID: 26437001
      20. CSF1R pathway activation was evident in the cHL and inactivation of this pathway could be a potential therapeutic target in cHL cases. PMID: 26066800
      21. All 4 hereditary diffuse leukoencephalopathy with axonal spheroids patients had a different single nucleotide mutation in the cytoplasmic part of the gene. Different mutations lead to different levels of depletion of nonclassical slan-positive monocytes. PMID: 26443621
      22. results suggest that CSF1R SNP rs10079250 may contribute to lung cancer susceptibility in never-smoking females PMID: 25144241
      23. Report treatment of diffuse-type tenosynovial giant cell tumour of the soft tissue using CSF1R inhibition with emactuzumab. PMID: 26179200
      24. Autocrine CSF1R signaling is essential in maintaining low claudin expression. PMID: 25088194
      25. CSF1R gene had variations in genic regions that affected the association of RORalpha with its target binding site in vivo PMID: 25913741
      26. The first report of hereditary diffuse leukoencephalopathy with neuroaxonal spheroids due to a novel CSF1R missense mutation. PMID: 25012610
      27. A missense mutation c.2563C>A (p.P855T) of the CSF1R gene has been identified to associated with hereditary diffuse leukoencephalopathy. PMID: 25863088
      28. CSF-1R D802V and KIT D816V homologous mutations have differential effects on receptor tertiary structure and allosteric communication. PMID: 24828813
      29. C/EBPalpha-C(m)-mediated downregulation of Csf1r has a negative, rather than positive, impact on the progression of AML involving C/EBPalpha-C(m), which might possibly be accelerated by additional genetic and/or epigenetic alterations inducing Csf1r upregulation PMID: 25534203
      30. The survival of CSF1R(pos) cells requires active AKT (v-akt murine thymoma viral oncogene homolog 1) signaling, which contributed to increased levels of nuclear, transcriptionally competent beta-catenin. PMID: 24722292
      31. CSF1R gene analysis was performed in 15 patients with undefined leukoencephalopathy and progressive cognitive decline PMID: 24532199
      32. We report three patients with HDLS who carried missense mutations in the CSF1R gene, two of them novel (p.L582P and p.V383L). PMID: 24706185
      33. The identified isoform of CSF-1R mRNA may interfere with the expression of full-length CSF-1R mRNA, thereby affecting the biological activity of the ligand/receptor signaling axis in Sprague-Dawley rats. PMID: 24682770
      34. CSF-1R mayact as a transcriptional regulator on proliferation-related genes in breast cancer. PMID: 24362524
      35. Our results provide new insights into the molecular physiology of the CSF-1 receptor and contribute to our understanding of substrate selection by TACE and gamma-secretase. PMID: 24955855
      36. haploinsufficiency of CSF-1R is sufficient to cause Hereditary diffuse leukoencephalopathy with spheroids [review] PMID: 24807373
      37. CSF1R mutations in hereditary diffuse leukoencephalopathy with spheroids are loss of function. PMID: 24145216
      38. diagnosis of HDLS or a de novo mutation in CSF1R must be considered in patients with bilateral symmetric changes in ukodystrophies. PMID: 24034409
      39. Data indicate that anti-CD115 monoclonal antibody H27K15 exerts partial inhibitory effects on CD115 signaling, but inhibits monocyte chemotactic protein-1 secretion and reduces interleukin-6 production. PMID: 23924795
      40. Our data demonstrate that a high number of non-Hodgkin/Reed-Sternberg cells expressing CSF-1R are correlated with an increased tumor macrophage content and worse survival in classical Hodgkin lymphoma. PMID: 24619759
      41. Our report emphasizes the presence of atypical Parkinsonism in Hereditary diffuse leukoencephalopathy with spheroids due to CSF1R mutations PMID: 23787135
      42. our study indicates that pathogenic mutations in CSF1R are an unlikely cause of multipel sclerosis in the Canadian population PMID: 23889897
      43. This study showed that a novel A781V mutation in the CSF1R gene causes hereditary diffuse leucoencephalopathy with axonal spheroids. PMID: 23816250
      44. CSF-1R signaling by haploinsufficiency may play a role in microglial dysfunction leading to the pathogenesis of hereditary diffuse leukoencephalopathy with spheroids PMID: 24336230
      45. These results indicate that all of the Fms mutations tested severely impair the kinase activity and most of the mutations also impair the trafficking to the cell surface, further suggesting that hereditary diffuse leukoencephalopathy with spheroids is caused by the loss of Fms function. PMID: 24120500
      46. One mechanism of RANK inhibition by 1,25(OH)2D3 is down-regulation of the M-CSF receptor c-Fms, which is required for the expression of RANK. PMID: 23116709
      47. Data suggest that CSF-1R-independent actions of IL-34 via receptor-type protein-tyrosine phosphatase zeta (PTP-zeta) might be considered in evaluating IL-34 roles in development and disease. PMID: 23744080
      48. CSF1R mutations are responsible for a significant proportion of clinically and pathologically proven hereditary diffuse leukoencephalopathies with spheroids. PMID: 23649896
      49. This study showed that Adult-onset leukoencephalopathy with neuroaxonal spheroids and pigmented glia with CSF1R mutation. PMID: 23052599
      50. CSF1/CSF1R signaling is important in the recruitment of tumor-infiltrating myeloid cells that can limit the efficacy of radiotherapy PMID: 23418320

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    • 相关疾病:
      Leukoencephalopathy, diffuse hereditary, with spheroids (HDLS)
    • 亚细胞定位:
      Cell membrane; Single-pass type I membrane protein.
    • 蛋白家族:
      Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
    • 组织特异性:
      Expressed in bone marrow and in differentiated blood mononuclear cells.
    • 数据库链接:

      HGNC: 2433

      OMIM: 164770

      KEGG: hsa:1436

      STRING: 9606.ENSP00000286301

      UniGene: Hs.586219



    1v1周青梅郑宣木天寥
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