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    Recombinant Bovine Cyclic AMP-dependent transcription factor ATF-4(ATF4)

    • 货号:
      CSB-YP669151BO
    • 规格:
    • 来源:
      Yeast
    • 其他:
    • 货号:
      CSB-EP669151BO
    • 规格:
    • 来源:
      E.coli
    • 其他:
    • 货号:
      CSB-EP669151BO-B
    • 规格:
    • 来源:
      E.coli
    • 共轭:
      Avi-tag Biotinylated

      E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

    • 其他:
    • 货号:
      CSB-BP669151BO
    • 规格:
    • 来源:
      Baculovirus
    • 其他:
    • 货号:
      CSB-MP669151BO
    • 规格:
    • 来源:
      Mammalian cell
    • 其他:

    产品详情

    • 纯度:
      >85% (SDS-PAGE)
    • 基因名:
      ATF4
    • Uniprot No.:
    • 别名:
      ATF4; Cyclic AMP-dependent transcription factor ATF-4; cAMP-dependent transcription factor ATF-4; Activating transcription factor 4
    • 种属:
      Bos taurus (Bovine)
    • 蛋白长度:
      full length protein
    • 表达区域:
      1-348
    • 氨基酸序列
      MAEMSFLSSE VLGGDFVSPF DQLGLGAEES LGLLDDNLEV AKHFKHHGFS CDKAKAGSSE WLAVDWLVSD NSKEDAFSGT DWMVEKMDLK EFDFDILFSK DDLETMPDEL LATLDDTCDL FQPLVQETNK EPPQIVNPIG HLPEGLPTID QGAPFTFFQP LPPSPGTLSS TPDHSFSLEL CSEVVIPEGD SKPDSTTTGF PQCIKEEDAP SDNDSGICMS PDSSLGSPQD SPSTSRGSPN KSLLSPGALS GSSRPKPYDP PGEKMVAAKV KGEKLDKKLK KMEQNKTAAT RYRQKKRAEQ EALTGECKEL EKKNEALKEK ADSLAKEIQY LKDQIEEVRK AREKKRVL
    • 蛋白标签:
      The following tags are available.
      N-terminal His-tagged
      Tag-Free
      The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
    • 产品提供形式:
      Lyophilized powder
      Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
    • 复溶:
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
    • 储存条件:
      Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
    • 保质期:
      The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
      Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
    • 货期:
      Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
      Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
    • 注意事项:
      Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
    • Datasheet :
      Please contact us to get it.

    产品评价

    靶点详情

    • 功能:
      Transcription factor that binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3') and displays two biological functions, as regulator of metabolic and redox processes under normal cellular conditions, and as master transcription factor during integrated stress response (ISR). Binds to asymmetric CRE's as a heterodimer and to palindromic CRE's as a homodimer. Core effector of the ISR, which is required for adaptation to various stress such as endoplasmic reticulum (ER) stress, amino acid starvation, mitochondrial stress or oxidative stress. During ISR, ATF4 translation is induced via an alternative ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced ATF4 acts as a master transcription factor of stress-responsive genes in order to promote cell recovery. Promotes the transcription of genes linked to amino acid sufficiency and resistance to oxidative stress to protect cells against metabolic consequences of ER oxidation. Activates the transcription of NLRP1, possibly in concert with other factors in response to ER stress. Activates the transcription of asparagine synthetase (ASNS) in response to amino acid deprivation or ER stress. However, when associated with DDIT3/CHOP, the transcriptional activation of the ASNS gene is inhibited in response to amino acid deprivation. Together with DDIT3/CHOP, mediates programmed cell death by promoting the expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the terminal unfolded protein response (terminal UPR), a cellular response that elicits programmed cell death when ER stress is prolonged and unresolved. Together with DDIT3/CHOP, activates the transcription of the IRS-regulator TRIB3 and promotes ER stress-induced neuronal cell death by regulating the expression of BBC3/PUMA in response to ER stress. May cooperate with the UPR transcriptional regulator QRICH1 to regulate ER protein homeostasis which is critical for cell viability in response to ER stress. In the absence of stress, ATF4 translation is at low levels and it is required for normal metabolic processes such as embryonic lens formation, fetal liver hematopoiesis, bone development and synaptic plasticity. Acts as a regulator of osteoblast differentiation in response to phosphorylation by RPS6KA3/RSK2: phosphorylation in osteoblasts enhances transactivation activity and promotes expression of osteoblast-specific genes and post-transcriptionally regulates the synthesis of Type I collagen, the main constituent of the bone matrix. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. Mainly acts as a transcriptional activator in cellular stress adaptation, but it can also act as a transcriptional repressor: acts as a regulator of synaptic plasticity by repressing transcription, thereby inhibiting induction and maintenance of long-term memory. Regulates synaptic functions via interaction with DISC1 in neurons, which inhibits ATF4 transcription factor activity by disrupting ATF4 dimerization and DNA-binding.
    • 亚细胞定位:
      Nucleus. Nucleus speckle. Cytoplasm. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
    • 蛋白家族:
      BZIP family
    • 数据库链接:


    1v1周青梅郑宣木天寥
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