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    Recombinant Arabidopsis thaliana Potassium channel AKT2/3(AKT2) ,partial

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    • 共轭:
      Avi-tag Biotinylated

      E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

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    • 来源:
      Mammalian cell
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    • 纯度:
      >85% (SDS-PAGE)
    • 基因名:
    • Uniprot No.:
    • 别名:
      AKT2; AKT3; At4g22200; T10I14.30Potassium channel AKT2/3
    • 种属:
      Arabidopsis thaliana (Mouse-ear cress)
    • 蛋白长度:
    • 蛋白标签:
      The following tags are available.
      N-terminal His-tagged
      The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
    • 产品提供形式:
      Lyophilized powder
      Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
    • 复溶:
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
    • 储存条件:
      Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
    • 保质期:
      The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
      Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
    • 货期:
      Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
      Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
    • 注意事项:
      Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
    • Datasheet :
      Please contact us to get it.



    • 功能:
      Highly selective and weak inward-rectifying potassium channel. Plays a role in both loading and unloading potassium into/from the phloem sap. Seems to control sugar loading into phloem via a voltage-dependent process. Blocked by physiological concentrations of external calcium and by external acidification. May interact with the cytoskeleton or with regulatory proteins. Dephosphorylation by PP2CA not only leads to the inhibition of potassium currents but also to an increase of the voltage-dependence of the channel. Regulated by the CBL4/CIPK6 calcium sensor/protein kinase complex via a kinase interaction-dependent but phosphorylation-independent translocation of the channel to the plasma membrane.
    • 基因功能参考文献:
      1. the outward-rectifying potassium-selective channel GORK limits the action potential amplitude and duration, while the weakly-rectifying channel AKT2 affects membrane excitability. PMID: 29561764
      2. AKT2 subunit containing K(+) channels mediate sodium chloride-induced depolarization of root cells. PMID: 27043750
      3. CBL4 together with the interacting protein kinase CIPK6 modulates the activity and plasma membrane targeting of the K(+) channel AKT2. PMID: 21445098
      4. Data discuss the existence of multiple post-translational modification steps that work in concert to convert AKT2 from an inward-rectifying into a non-rectifying K (+) channel. PMID: 21445013
      5. the AKT2/3 subunit constitutes the Ca2+ sensitivity of the guard cell K+ uptake channel PMID: 15824192
      6. The role of 2 phosphorylation sites on the switch between gating modes of AKT2 are described. PMID: 16297070
      7. AKT2-transformed tobacco mesophyll cells displayed inward-rectifying, rather than voltage-independent K+ channels. PMID: 17146665



    • 亚细胞定位:
      Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Targeted to the cell membrane when interacting with CIPK6 and CBL4.
    • 蛋白家族:
      Potassium channel family, Plant (TC 1.A.1.4) subfamily
    • 组织特异性:
      Expressed mainly in the phloem tissues throughout the plant but also, at a lower level, in leaf epiderm, mesophyll and guard cells.
    • 数据库链接:

      KEGG: ath:AT4G22200

      STRING: 3702.AT4G22200.1

      UniGene: At.1970

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