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  • Your Good Partner in Biology Research

    ITK Antibody

    Datasheet
    • 货号:
      CSB-RA954682A0HU
    • 规格:
      ¥2200
    • 图片:
      • IHC image of CSB-RA954682A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • 其他:

    产品详情

    • 产品描述:

      ITK is a non-receptor tyrosine kinase that drives T-cell signaling downstream of TCR activation. It is highly expressed in T-cells. ITK signaling is involved in the regulation of T cell activation, T helper cell differentiation, and thymic selection of developing thymocytes. Human ITK deficiency is linked to primary immunodeficiency, progressive natural killer T (NKT) and CD4+ T-cell lymphopenia, increased Epstein-Bar virus (EBV) vulnerability, and EBV-driven lymphoproliferative disorders, with frequent pulmonary involvement emerging as a clinical signature. In addition to the involvement in the pathogenesis of autoimmune diseases, ITK also participates in carcinogenesis.

      Mammalian cells are transfected with plasma vectors containing ITK antibody genes, allowing for both recombinant ITK antibody expression and secretion to the medium. Collecting the cell supernatant and purifying to obtain the recombinant ITK antibody by Affinity-chromatography. This recombinant ITK antibody has been validated to detect the ITK protein of Human in the ELISA, IHC.

    • Uniprot No.:
    • 基因名:
      ITK
    • 别名:
      Tyrosine-protein kinase ITK/TSK (EC 2.7.10.2) (Interleukin-2-inducible T-cell kinase) (IL-2-inducible T-cell kinase) (Kinase EMT) (T-cell-specific kinase) (Tyrosine-protein kinase Lyk), ITK, EMT LYK
    • 反应种属:
      Human
    • 免疫原:
      A synthesized peptide derived from human ITK
    • 免疫原种属:
      Homo sapiens (Human)
    • 标记方式:
      Non-conjugated
    • 克隆类型:
      Monoclonal
    • 抗体亚型:
      Rabbit IgG
    • 纯化方式:
      Affinity-chromatography
    • 克隆号:
      8F10
    • 浓度:
      It differs from different batches. Please contact us to confirm it.
    • 保存缓冲液:
      Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
    • 产品提供形式:
      Liquid
    • 应用范围:
      ELISA, IHC
    • 推荐稀释比:
      Application Recommended Dilution
      IHC 1:50-1:200
    • Protocols:
    • 储存条件:
      Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
    • 货期:
      Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

    产品评价

    靶点详情

    • 功能:
      Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Required for TCR-mediated calcium response in gamma-delta T-cells, may also be involved in the modulation of the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells. Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3.
    • 基因功能参考文献:
      1. These observations have significant therapeutic implications, as pharmacologic inhibition of ITK prevented the activation of this signaling axis and overcame chemotherapy resistance. PMID: 27780854
      2. Our results provide new insight into the effect of ITK and suboptimal T-cell receptor signaling on CD8(+) T cell function, and how these may contribute to phenotypes associated with ITK deficiency PMID: 28213500
      3. The data indicate that increased ITK expression could act as a disease activity marker and as a risk factor for involvement in SLE, but it still needs further study to confirm. PMID: 27729219
      4. We conclude that ITK, formerly considered an immune cell-specific protein, is aberrantly expressed in melanoma and promotes tumor development and progression PMID: 25934889
      5. The kinase Itk and the adaptor TSAd change the specificity of the kinase Lck in T cells by promoting the phosphorylation of Tyr192. PMID: 25492967
      6. Found that 38% and 14% of the Angioimmunoblastic T-cell lymphoma cases exhibited gains of ITK and SYK genes, respectively. PMID: 25337257
      7. These data indicate that PRN694 is a highly selective and potent covalent inhibitor of ITK and RLK, and its extended target residence time enables durable attenuation of effector cells in vitro and in vivo. PMID: 25593320
      8. approach identified 18 kinase and kinase-related genes whose overexpression can substitute for EGFR in EGFR-dependent PC9 cells, and these genes include seven of nine Src family kinase genes, FGFR1, FGFR2, ITK, NTRK1, NTRK2, MOS, MST1R, and RAF1. PMID: 25512530
      9. ITK deficiency is a genetic cause of idiopathic CD4+ T-cell lymphopenia. PMID: 25061172
      10. Data indicate reduced T-cell activation by altered IL-2 inducible T-cell kinase (ITK) expression in vitro. PMID: 24462896
      11. T-cell-specific human ITK-Syk oncogene in mice leads to early polyclonal T cell lymphoproliferation with B cell expansion. It induces terminal T cell differentiation via Blimp-1, eliminating oncogene-expressing cells early in development. PMID: 24376268
      12. Case Report/Letter: ITK/SYK translocation in angioimmunoblastic T-cell lymphoma. PMID: 24076779
      13. a new pathway regulated by Itk in cells, and suggest cross talk between Itk and G-protein signaling downstream of the TcR. PMID: 23454662
      14. Data indicate that the intracellular signaling of ITK-SYK requires both SLP-76 and the adapter function of SYK/ZAP-70 kinases. PMID: 23293025
      15. ITK and Gag colocalized at the plasma membrane and were concentrated at sites of F-actin accumulation and membrane lipid rafts in HIV-1 infected T cells PMID: 23260110
      16. DEF6, a novel substrate for the Tec kinase ITK, contains a glutamine-rich aggregation-prone region and forms cytoplasmic granules that co-localize with P-bodies. PMID: 22829599
      17. This review focuses on Itk and its role in regulating T-cell signaling and function, especially the activation and development of alpha-beta T cells. PMID: 22449075
      18. ITK mutations are distributed over the entire protein and include missense, nonsense and indel mutations, reminiscent of the situation in its sister kinase in B cells, Bruton's tyrosine kinase PMID: 22289921
      19. These results indicate that Itk is required for efficient replication of influenza virus in infected T-cells. PMID: 22302878
      20. No detrimental mutations were identified in ITK in Chinese children with Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis. PMID: 21674762
      21. These data suggest a sequential mechanism whereby ZAP-70-dependent priming of SLP-76 at three N-terminal sites triggers reciprocal regulatory interactions between Itk and SLP-76, which are ultimately required to couple active Itk to PLC-gamma1. PMID: 21725281
      22. People with the -rare allele 196C>T may be more susceptible to asthma via transcriptional regulation of the ITK gene. PMID: 21323647
      23. Knocking down Itk expression inhibits Jurkat cell proliferation and cytokines production. PMID: 21280324
      24. TSAd, through its interaction with both Itk and Lck, primes Itk for Lck mediated phosphorylation and thereby regulates CXCL12 induced T cell migration and actin cytoskeleton rearrangements PMID: 20305788
      25. T-cell receptor (TCR)-induced association between ITK and SLP-76, recruitment and transphosphorylation of ITK, actin polarization at the T-cell contact site, and expression of Th2 cytokines PMID: 20457812
      26. Expression of patient-derived ITK in mice induces highly malignant peripheral T cell lymphomas. PMID: 20439541
      27. Data show that tacrolimus modified the expression levels of Foxp3-regulated T cell receptor signal related-genes, PTPN22 and Itk, in Treg cells. PMID: 19714314
      28. Itk catalytic activity is inhibited by the peptidyl prolyl isomerase activity of cyclophilin A (CypA). PMID: 11830645
      29. In this review, the pathways are discussed by which Itk might impact the differentiation of T helper (Th) cells. PMID: 16931156
      30. Itk forms dimers in the membrane and that receptors that recruit Itk do so to specific membrane regions PMID: 17060314
      31. Vav phosphorylation correlates with calcium flux and Itk phosphorylation during mitogenic CD28 signalaing in Jurkat cells PMID: 17237383
      32. Active signals from transgenic Tec kinase (ITK) regulate the development of memory-like CD8+ T cells with innate function. PMID: 17724684
      33. Itk autophosphorylation on Y180 within the SH3 domain occurs exclusively via an intramolecular, in cis mechanism PMID: 17897671
      34. In transgenic mice specifically lacking Itk kinase activity, active kinase signaling is required for control of T helper (Th)2 cell responses and development of allergic asthma. PMID: 18322190
      35. These data suggest that inhibition of ITK blocks HIV infection by affecting multiple steps of HIV replication. PMID: 18443296
      36. Analysis of SNPs in TCR pathway genes revealed that a haplotype that covers a major part of the coding sequence of ITK is a risk factor for seasonal allergic rhinitis. PMID: 19222422
      37. ITK deficiency as a result of a R335W missense mutation, causes a novel immunodeficiency syndrome that leads to a fatal inadequate immune response to Epstein-Barr virus. PMID: 19425169
      38. The authors identify the residues on the surface of the Itk SH2 domain responsible for substrate docking and show that this SH2 surface mediates autophosphorylation in the full-length Itk molecule. PMID: 19523959
      39. Findings identify ITK-SYK as an active, transforming FTK and suggest ITK-SYK as a rational therapeutic target for t(5;9)(q33;q22)-positive lymphomas. PMID: 19535334
      40. these results provide an initial step in understanding the biological role of Itk-TFII-I signaling in T-cell function. PMID: 19701889
      41. The Tec family kinase Itk exists as a folded monomer in vivo. PMID: 19717557
      42. Studies of Itk deletion mutants indicate that the amino acids in the N-terminal 152 residues and proline-rich domains enhance catalysis by affecting turnover rate rather than by substrate binding. PMID: 11437596

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    • 相关疾病:
      Lymphoproliferative syndrome 1 (LPFS1)
    • 亚细胞定位:
      Cytoplasm. Nucleus. Note=Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.
    • 蛋白家族:
      Protein kinase superfamily, Tyr protein kinase family, TEC subfamily
    • 组织特异性:
      T-cell lines and natural killer cell lines.
    • 数据库链接:

      HGNC: 6171

      OMIM: 186973

      KEGG: hsa:3702

      STRING: 9606.ENSP00000398655

      UniGene: Hs.558348



    1v1周青梅郑宣木天寥
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