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  • Your Good Partner in Biology Research

    CRK Antibody

    Datasheet
    • 货号:
      CSB-PA005979LA01HU
    • 规格:
      ¥1320
    • 图片:
      • Western Blot
        Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate
        All lanes: CRK antibody at 3μg/ml
        Secondary
        Goat polyclonal to rabbit IgG at 1/50000 dilution
        Predicted band size: 34, 23 kDa
        Observed band size: 37 kDa
      • Immunofluorescent analysis of A549 cells using CSB-PA005979LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
    • 其他:

    产品详情

    • 产品名称:
      Rabbit anti-Homo sapiens (Human) CRK Polyclonal antibody
    • Uniprot No.:
    • 基因名:
      CRK
    • 别名:
      Adapter molecule crk antibody; Avian sarcoma virus CT10 (v crk) oncogene homolog antibody; CRK antibody; CRK isoform 2 antibody; CRK isoform II antibody; CRK_HUMAN antibody; CRKII antibody; FLJ38130 antibody; OTTHUMP00000115366 antibody; OTTHUMP00000198330 antibody; p38 antibody; Proto oncogene C crk antibody; Proto-oncogene C-crk antibody; v crk avian sarcoma virus CT10 oncogene homolog antibody; v crk sarcoma virus CT10 oncogene homolog antibody; v crk sarcoma virus CT10 oncogene homolog (avian) antibody
    • 宿主:
      Rabbit
    • 反应种属:
      Human, Rat
    • 免疫原:
      Recombinant Human Adapter molecule crk protein (40-196AA)
    • 免疫原种属:
      Homo sapiens (Human)
    • 标记方式:
      Non-conjugated

      本页面中的产品,CRK Antibody (CSB-PA005979LA01HU),的标记方式是Non-conjugated。对于CRK Antibody,我们还提供其他标记。见下表:

      可提供标记
      标记方式 货号 产品名称 应用
      HRP CSB-PA005979LB01HU CRK Antibody, HRP conjugated ELISA
      FITC CSB-PA005979LC01HU CRK Antibody, FITC conjugated
      Biotin CSB-PA005979LD01HU CRK Antibody, Biotin conjugated ELISA
    • 克隆类型:
      Polyclonal
    • 抗体亚型:
      IgG
    • 纯化方式:
      >95%, Protein G purified
    • 浓度:
      It differs from different batches. Please contact us to confirm it.
    • 保存缓冲液:
      Preservative: 0.03% Proclin 300
      Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
    • 产品提供形式:
      Liquid
    • 应用范围:
      ELISA, WB, IF
    • 推荐稀释比:
      Application Recommended Dilution
      WB 1:500-1:5000
      IF 1:50-1:200
    • Protocols:
    • 储存条件:
      Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
    • 货期:
      Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

    产品评价

    靶点详情

    • 功能:
      Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1.; Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.
    • 基因功能参考文献:
      1. A reduced miR-126 expression and an elevated Crk protein expression, alone or in combination, statistically correlated with aggressive clinicopathological characteristics, such as larger tumor size, deeper local invasion, more lymph node metastasis, advanced TNM stage, and poorer prognosis. PMID: 29171987
      2. these data highlight a signaling pathway in which SHP-1 acts through CrkII to reshape the pattern of Rap1 activation in the immunological synapse. PMID: 28790195
      3. Crk-dependent increased phosphorylation of CD3zeta coincided with inhibition of TCR downmodulation, supporting a positive role for Crk adaptor proteins in TCR-mediated signal amplification. PMID: 28465009
      4. This mini review will focus on the emerging roles of Crk adaptors during myocardial I/R injury. PMID: 28553779
      5. TGF-beta and Crk collaborate to form a positive feedback loop to facilitate epithelial-mesenchymal transition (EMT). PMID: 27027347
      6. The results suggest the involvement of Crk adaptor proteins in the early stages of T cell activation in which Crk might help recruiting effector proteins to the vicinity of the phospho-CD3zeta and contribute to the fine-tuning of the TCR/CD3-coupled signal transduction pathways. PMID: 28526413
      7. Study investigated structures and thermodynamics of the interactions mediated by N-terminal Src homology 3 domain of CrkII and proline-rich motifs (PRMs) of cAbl kinase, highlight the role of interfacial water molecules and the conformational entropy in the SH3:PRM interactions PMID: 27332121
      8. CrkII mediates IGF-1 signaling and further balances pancreatic ductal adenocarcinoma biological behaviors via Erk1/2 and Akt pathway PMID: 26250463
      9. In individuals with class I 17p13.3 microduplications including CRK, we recommend biochemical evaluation of the growth hormone axis. Providers caring for these patients should be aware of their possible risk for the development of central precocious puberty. PMID: 27633569
      10. Crk Tyr251 phosphorylation regulate invasive cell phenotypes in glioblastoma. PMID: 26473374
      11. CsA and FK506 might interfere with selected effector T cell functions via a CrkII-, but not CrkI-dependent mechanisms. PMID: 26792730
      12. Studied the role of PAK1/Crk axis in transduction of the oncogenic KRAS signal in non-small cell lung cancer (NSCLC). PMID: 25956913
      13. in human c-CrkII, movement at position 239 and strain at position 272 are not tolerated because the beta-branched residues Ile239 and Val272 restrain the backbone mobility and thus destabilize the cis Pro238 form. PMID: 26456136
      14. Data indicate the role of tyrosine phosphorylation in regulating modular domain utilization in v-crk sarcoma virus CT10 oncogene homolog (avian) protein (Crk). PMID: 25381819
      15. evidence uncovered essential roles of CRK in invasive bladder cancer through the hepatocyte growth factor/c-Met/CRK feedback loop for epithelial-mesenchymal transition induction PMID: 25816892
      16. In this review, we describe the structure and function of the different Crk proteins. PMID: 25506591
      17. proline switch and its role in Crk signaling PMID: 24702481
      18. Data indicate that human proto-oncogene c-CrkII (CrkII) is regulated by phosphorylation only, but chicken CrkII is regulated by Pro238 trans-->cis isomerization and by Tyr222 phosphorylation. PMID: 25284755
      19. Study indicates that miR-126 is a tumor suppressor that inhibits gastric cancer cells proliferation by targeting PI3KR2, Crk and PLK2. PMID: 24969300
      20. Crk1/2 and CrkL are physically linked, functionally complement each other during podocyte foot process spreading, and together are required for developing typical foot process architecture. PMID: 24499776
      21. The present data demonstrate that immunophilins regulate CrkII, but not CrkI activity in T cells and suggest that CsA and FK506 inhibit selected effector T cell functions via a CrkII-dependent mechanism. PMID: 25225668
      22. This study analyzed the kinetics of folding and substrate binding of the two homologues and found that cis-trans isomerization of Pro238 determines target binding in chicken but not in human CT10 regulator of kinase protein (c-CrkII). PMID: 24571054
      23. We propose that Crk adaptor proteins inhibit actin polymerization by competing with Nck binding to Tir. PMID: 24675776
      24. This prospect summarizes recent developments in Crk biology, including new structural and biochemical roles for the atypical carboxyl-terminal SH3 (SH3C) domain. PMID: 24356912
      25. These findings suggest that PTPN4 negatively regulates cell proliferation and motility through dephosphorylation of CrkI. PMID: 23666597
      26. P-glycoprotein activity in association with pCrkl reduction levels might help to distinguish between imatinib-resistant and imatinib-sensitive chronic myeloid leukemia cells. PMID: 24210993
      27. A gene signature derived following CrkII over-expression correlated significantly with basal breast cancers and with high grade and poor outcome in general; data highlight the physiological importance of Crk proteins in regulating growth of aggressive basal breast cancer cells PMID: 22569336
      28. the role of PAK1 in the promotion of cell motility, cell invasiveness and the down regulation of p120-catenin through CRK serine 41 phosphorylation in NSCLC cells PMID: 22848689
      29. SRC, c-MET and CRK play a key role in gastric carcinogenesis by modulating CagA signal transductions and interaction between CRK gene and phytoestrogens modify gastric cancer risk PMID: 22383989
      30. miR-126 may play an initial role in the development and progression of EMs. Crk may be regulated by miR-126, and synergism between abnormal expressions may play an important role in the pathogenesis of EMs. PMID: 22012249
      31. propose a unified model for inhibitory receptor function: Crk phosphorylation prevents essential Crk-dependent activation signals and blocks F-actin network formation, thereby reducing constraints on subsequent engagement of activation receptors PMID: 22464172
      32. CrkI and p130(Cas) complex regulates the migration and invasion of prostate cancer cells. PMID: 22144090
      33. positive regulatory role resulting from tyrosine phosphorylation of Crk, and identify a novel mechanism by which an adaptor protein activates a non-receptor tyrosine kinase by SH2 domain displacement PMID: 21602891
      34. differential binding selectivity of Crk and CrkL to their downstream partners Dock 180 and C3G was demonstrated in ovarian cancer cell line SKOV3 through coimmunoprecipitation PMID: 21319228
      35. CRKII increases migration and invasive potential in oral squamous cell carcinoma PMID: 21339045
      36. SOS1 is essential for CrkI-induced fibroblast transformation, and also reveal a surprising negative role for Abl kinases in Crk transformation. PMID: 20729917
      37. These results imply that binding capacity of influenza A virus NS1 to CRK/CRKL has evolved in virus strains that over-induce the antiviral acting JNK-ATF2 signalling module and helps to suppress the detrimental apoptosis promoting action of this pathway. PMID: 20088952
      38. signal transfer of Crk/Dock180/Rac1 is implicated in actin cytoskeleton reorganization and thus in the cell proliferation, motility, invasion, and of human ovarian cancer cell line SKOV3. PMID: 20237902
      39. Silencing of CRK-I/II increased endothelial cell migration. PMID: 19760510
      40. Depletion of Crk by RNA interference markedly inhibited proliferation of the synovial sarcoma cell lines HS-SYII, SYO-1, and Fuji as well as prevented anchorage-independent growth. PMID: 19737974
      41. Molecular and immunohistochemical analysis of signaling adaptor protein Crk in human cancers PMID: 11911970
      42. determined the NMR solution structure of the ternary complex of the Abl SH3 domain with the Crk SH2 domain bound to a Crk pY221 phosphopeptide PMID: 12384576
      43. Crk plays a critical role in Rac1-induced membrane ruffling and Rap1-mediated nascent focal complex stabilization contributing to ephrin-B1-induced human aortic endothelial cells migration PMID: 12475948
      44. Requirement of the p130CAS-Crk coupling for metastasis suppressor KAI1/CD82-mediated inhibition of cell migration in a metastatic prostate cancer cell line. PMID: 12738793
      45. CRK is ADP ribosylated by Pseudomonas aeruginosa ExoT, demonstrating how ADP-ribosylation of Crk-I may be responsible for the anti-phagocytosis phenotype elicited by ExoT in mammalian cells PMID: 12807879
      46. c-Src phosphorylates the receptor KIT, thereby creating docking sites for SH2 domain containing proteins, leading to recruitment of Crk to the receptor. PMID: 12878163
      47. This study provides the first quantitative analysis of discrete CRKI and CRKII protein isoforms in human lung tumors and provides evidence that the C-CRK proto-oncogene may foment a more aggressive phenotype in lung cancers. PMID: 12970743
      48. Nck and Crk mediate distinct VEGF-induced signaling pathways that serve overlapping functions in cell migration. PMID: 15051508
      49. a major role for a Crk-cortactin complex in actin polymerization downstream of tyrosine kinase signaling PMID: 15263018
      50. Crk may be involved in regulation of cell motility by a hyaluronic acid-dependent mechanism through an association with ERMs PMID: 15326184

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    • 亚细胞定位:
      Cytoplasm. Cell membrane.
    • 蛋白家族:
      CRK family
    • 数据库链接:

      HGNC: 2362

      OMIM: 164762

      KEGG: hsa:1398

      STRING: 9606.ENSP00000300574

      UniGene: Hs.461896



    1v1周青梅郑宣木天寥
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