Rat keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Rat AREG ELISA Kit was designed for the quantitative measurement of Rat AREG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 pg/mL-80 pg/mL and the sensitivity is 0.31 pg/mL .
别名：

Areg; Sdgf; Amphiregulin; AR; Schwannoma-derived growth factor; SDGF
缩写：

AREG
Uniprot No.：

P24338
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.25 pg/mL-80 pg/mL
灵敏度：

0.31 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich
说明书：

下载最新说明书

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	95
	Range %	91-99
1:4	Average %	86
	Range %	80-92
1:8	Average %	96
	Range %	92-102

回收率：

The recovery of rat KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	98-108
EDTA plasma (n=4)	97	92-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
基因功能参考文献：
1. Report increased AREG transcript and protein levels in the lungs of rodents with DA-induced bronchiolitis obliterans. PMID: 24816162
2. estrogen and progeterone increased cell proliferation in normal gland and mammary cancers through the increased production of amphiregulin PMID: 21258428
3. may play a role in the ovulatory process of cycling rats as well as in the induction of ovulation in immature rats PMID: 15291759
4. the expression of amphiregulin (AR), a member of the epidermal growth factor (EGF) family, is rapidly and highly up-regulated by PTH in several osteoblastic cell lines and bone tissues PMID: 15509566
5. findings identify Amphiregulin as a factor for resistance of glioma cells to delta9-Tetrahydrocannabinol induced apoptosis PMID: 19229996
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亚细胞定位：

Membrane; Single-pass membrane protein.
蛋白家族：

Amphiregulin family
数据库链接：

KEGG: rno:29183

STRING: 10116.ENSRNOP00000003703

UniGene: Rn.10568