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  • Your Good Partner in Biology Research

    Rat Testosterone,T ELISA Kit

    • 中文名稱:
      大鼠睪酮(T)ELISA Kit
    • 貨號:
      CSB-E05100r
    • 規格:
      96T/48T
    • 價格:
      ¥3900/¥2500
    • 其他:

    產品詳情

    • 產品描述:

      This Rat Testosterone (T) ELISA Kit was designed for the quantitative measurement of Rat Testosterone (T) protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.13 ng/mL-25.6 ng/mL and the sensitivity is 0.06 ng/mL.

    • 別名:
      N/A
    • 縮寫:
      T
    • 種屬:
      Rattus norvegicus (Rat)
    • 樣本類型:
      serum, plasma, cell culture supernates, tissue homogenates
    • 檢測范圍:
      0.13 ng/mL-25.6 ng/mL
    • 靈敏度:
      0.06 ng/mL
    • 反應時間:
      1-5h
    • 樣本體積:
      50-100ul
    • 檢測波長:
      450 nm
    • 研究領域:
      Signal Transduction
    • 測定原理:
      quantitative
    • 測定方法:
      Competitive
    • 說明書:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<15%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<15%      
      Three samples of known concentration were tested in twenty assays to assess.    
                   
    • 線性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of rat testosteronen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
        Sample Serum(n=4)  
      1:1 Average % 91  
      Range % 86-96  
      1:2 Average % 97  
      Range % 92-104  
      1:4 Average % 85  
      Range % 81-88  
      1:8 Average % 89  
      Range % 83-93  
    • 回收率:
      The recovery of rat testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 90 87-95  
      EDTA plasma (n=4) 94 90-98  
                   
                   
    • 標準曲線:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      ng/ml OD1 OD2 Average    
      25.6 0.238 0.236 0.237    
      6.4 0.435 0.431 0.433    
      2 0.611 0.615 0.613    
      0.5 0.984 0.982 0.983    
      0.13 1.403 1.398 1.401    
      0 2.001 2.003 2.002    
    • 本試劑盒所含材料:
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit Testosterone antibody.
      • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Anti-Testosterone Antibody 1 x 6 ml -- Act as the detection antibody.
      • HRP-conjugated Testosterone 1 x 6 ml -- Bind to the DHT antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
      • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
      • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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    • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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    • 數據處理:
    • 貨期:
      3-5 working days

    引用文獻

    產品評價



    1v1周青梅郑宣木天寥
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