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    4. Rat TNF-α ELISA kit

    Rat TNF-α ELISA kit

    • 中文名称：
      大鼠肿瘤坏死因子α(TNF-α)ELISA kit
    • 货号：
      CSB-E11987r
    • 规格：
      96T/48T
    • 价格：
      ￥3800/￥2500
    • 其他：

    产品详情

    • 产品描述：

      This Rat TNF ELISA Kit was designed for the quantitative measurement of Rat TNF protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .

    • 别名：
      Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
    • 缩写：
      TNF
    • Uniprot No.：
    • 种属：
      Rattus norvegicus (Rat)
    • 样本类型：
      serum, plasma, cell culture supernates, tissue homogenates
    • 检测范围：
      6.25 pg/mL-400 pg/mL
    • 灵敏度：
      1.56 pg/mL
    • 反应时间：
      1-5h
    • 样本体积：
      50-100ul
    • 检测波长：
      450 nm
    • 研究领域：
      Immunology
    • 测定原理：
      quantitative
    • 测定方法：
      Sandwich
    • 说明书：
      下载最新说明书
    • 精密度：
      Intra-assay Precision (Precision within an assay): CV%<8%
      Three samples of known concentration were tested twenty times on one plate to assess.
      Inter-assay Precision (Precision between assays): CV%<10%
      Three samples of known concentration were tested in twenty assays to assess.
    • 线性度：
      To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      　SampleSerum(n=4)
      1:1Average %90
      Range %87-96
      1:2Average %99
      Range %95-104
      1:4Average %101
      Range %97-105
      1:8Average %98
      Range %93-107
    • 回收率：
      The recovery of rat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample TypeAverage % RecoveryRange
      Serum (n=5) 8580-92
      EDTA plasma (n=4)9995-104
    • 标准曲线：
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      pg/mlOD1OD2AverageCorrected
      4002.037 2.067 2.052 1.871
      2001.445 1.489 1.467 1.286
      1001.017 1.034 1.026 0.845
      500.655 0.677 0.666 0.485
      250.411 0.438 0.425 0.244
      12.50.351 0.378 0.365 0.184
      6.250.297 0.282 0.290 0.109
      00.183 0.179 0.181 　
    • 数据处理：
      ELISA操作视频及标曲绘制软件下载
    • 货期：
      3-5 working days

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    Tnf Proteins

    问答及客户评论

    ■ 常见问题解答
    Q:

    Seeking for ELISA kits of TNF a .I am working on SD Rats and project related to High Fat Diet induced Metabolic complications.

    A:
    Thanks for your inquiry.
    For the mentiond detection targets,we have the following kit:
    #CSB-E11987r,Rat TNF-α ELISA kit
    And we use kits to test ordinary rats.While there is not big problems to detect SD Rats.Pls take your actual test results as the real results
    Q:

    Rat TNF-α ELISA kit -CSB-E11987r this kit can be used for estimation in BALF...fluid
    Kindly answer me.

    A:
    Thanks for your enquiry.
    We usually use this kit to test serum, plasma, cell culture supernates, tissue homogenates.If you want to test bronchoalveolar lavage fluid,we suggest you do pre-testing first.If the content of your sample falls in the detection range,it can usually be detected out.
    The manual will be provided to you by e-mail.
    Pls let me know if you have any further questions. Thank you.
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    ■ 客户评论
    • Sample type: PVN tissue
      Sample species: Rat
      Review: The cytokine levels of IL-1β(F) and TNF-α(G) from the PVN tissue as measured by ELISA. n = 10–11 for each group. *P < 0.01 and **P < 0.05 versus sham groups; &P < 0.01 and #P < 0.05 versus MI + shCtrl group.
      PMID: 30978539
    • Sample type: Myocardial cells
      Sample species: Rat
      Review: TNF-α protein level in the conditioned media.#P < 0.05 vs Control group; *P < 0.05 vs Ang II group. The statistical analyses were performed using one-way ANOVA followed by Bonferroni's post hoc test. n = 3 independent experiments.
      PMID: 30953402
    • Sample type: Serum and lung tissue
      Sample species:Rat
      Review: MSCs-HO-1 decreased LPS-mediated tumor necrosis factor α (TNF-α; a) and interleukin 1β (IL-1β; b) in sera of all groups at the indicated time points. (c, d) MSCs-HO-1 decreased LPS-induced TNF-α (c) and IL-1β (d) in lungs of all groups at the indicated time points. All data are expressed as mean ± SEM (n = 6). *p < 0.05 versus 0 day time point, and #p < 0.05 versus lenti-HO-1-GFP and MSC groups at each time point.
      PMID: 30362554
    • Sample type: Serum
      Sample species: Rat
      Review: Effect of GABARAP and DCs on cartilage appearance and the expression of inflammatory cytokines in serum. In vivo cartilagerepair at 4 and 8 weeks postsurgery, (a) Macroscopic appearance and (b) macroscopic scores of femoral condyles were detected. Meanwhile,serum levels of (c) IL-6, (d) TNF-α, and (e) MMP-13 were determined using ELISA kits. Values are the means ± SD (n = 12). Bars with different letters are significantly different from each other at p < 0.05 and those with the same letter exhibit no significant difference. DC: differentiated chondrocytes; ELISA: enzyme-linked immunosorbent assay; GABARAP: γ-aminobutyric acid receptor-associated protein; SD: standard deviation [Color figure can be viewed at wileyonlinelibrary.com].
      PMID: 31020644
    • Sample type: Colorectal tissues
      Sample species: Rat
      Review: Effects of BD-SNEDDS on colon levels of inflammatory cytokines and oxidative stress.
      PMID: 30319255
    • Sample type: Liver homogenate
      Sample species: Rat
      Review: Effects of MFKE on proinflammatory biomarkers in rats treated with APAP-induced liver toxicity. (A) MRNA expressions of tumour necrosis factor- , interlukin-1 and inducible nitric oxide synthase.
      PMID: 30823534
    • Sample type: Serum
      Sample species: Rat
      Review: Serum concentrations of biomarkers, including TGF-β1, TNFα, IL-1β, IL-6, IL-18, CRP, and BNP were measured by Elisa kits following manufacture instructions.
      PMID: 30466624
    • Sample type: Cell culture supernates
      Sample species: Rat
      Review:The release of TNF-α. Single asterisk refers to p < 0.05 and double asterisks refer to p < 0.01.
      PMID: 30675709
    • Sample type: Spinal cord tissue
      Sample species: Rat
      Review: The concentrations of inflammatory cytokines IL-6 (a), TNF-α (b), and IL-1β (c) and anti‐inflammatory cytokines TGF-β (d) and IL‐13 (e) were measured by ELISA in sham-operated rats (n = 6) and in SCI rats treated with melatonin (n = 6) or vehicle (n = 6). Data are presented as mean ± SEM, ***p < 0.001: SCI + Vehicle versus sham; ### p < 0.001: SCI + Melatonin versus SCI + Vehicle, and One‐way ANOVA.
      PMID: 31006904
    • Sample type: Bronchoalveolar lavage fluid
      Sample species: Rat
      Review: Levels of TNF-α, IL-6, IL-8, and IL-10 detected by ELISA. n =15 in each group; The measurement data were expressed by mean ± standard deviation, one-way ANOVA was used for comparison between groups and the survival rate of 0~120 h in each group was compared by Kaplan-Meier analysis; *, p < 0.05 when compared with the normal group; #, p <0.05 when compared with the ALI + PBS group; eMSCs, mesenchymal stem cell; ALI, acute lung injury; OS, oxidative stress; TNF, tumor necrosis factor; IL, interleukin; SOD, superoxide dismutase; MDA, methylenedioxyamphetamine; GSH, glutathione; BALF, bronchoalveolar lavage fluid; ELISA, enzyme linked immunosorbent assay; HE, hematoxylin-eosin; ANOVA, analysis of variance.
      PMID: 30892077
    • Tested application: ELISA
      Test sample: Serum,Tissue homogenate: heart tissue homogenate
      Species: Rat
      Review: The standard was worked perfectly in my experiment but I could don get much difference in control and disease group animal serum samples.
    • Sample type: Serum
      Sample species: Rat
      Review: The results were remarkable and the repeatability of the kit was phenomenal.

    靶点详情

    • 功能：
      Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
    • 亚细胞定位：
      Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
    • 蛋白家族：
      Tumor necrosis factor family
    • 数据库链接：

      KEGG: rno:103694380

      STRING: 10116.ENSRNOP00000001110

      UniGene: Rn.2275

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