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  • Your Good Partner in Biology Research

    Rat Interleukin 1β,IL-1β ELISA Kit

    • 中文名称:
      大鼠白介素1β (IL-1β)ELISA Kit
    • 货号:
      CSB-E08055r
    • 规格:
      96T/48T
    • 价格:
      ¥3200/¥2500
    • 其他:

    产品详情

    • 产品描述:

      CUSABIO's IL1B rat ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL1B in serum, plasma, or tissue homogenates. It has good reproducibility, high specificity & sensitivity, and good linearity. This assay uses an antibody specific for rat IL1B coated on a 96-well plate. IL1B present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated IL1B antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1B bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader. This IL1B rat ELISA kit has been described in up to 150 publications.

      IL1B is a potent pro-inflammatory cytokine essential for host-defense responses to infection and injury. IL1B binds to IL1RI and then associates with the IL-1RAcP, initiating the recruitment of the MyD88 adaptor and activation and ligation to IRAK4, activating the MAPK p38 cascade and the NF-κB signaling pathways that are implicated in inflammatory responses. It is involved in pain, inflammation, and autoimmune disorders. IL1B also exerts important homeostatic effects in the normal organism, including the modulation of temperature, feeding, and sleep. Aberrant expression of IL1B has been found in various fibrotic diseases. For example, IL1B overexpression participates in the pathophysiological changes that occur during different disease states, such as rheumatoid arthritis, osteoarthritis, and Alzheimer's disease.

    • 别名:
      Il1bInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
    • 缩写:
      IL1B
    • Uniprot No.:
    • 种属:
      Rattus norvegicus (Rat)
    • 样本类型:
      serum, plasma, tissue homogenates.
    • 检测范围:
      0.156 ng/ml-10 ng/ml.
    • 灵敏度:
      0.039 ng/ml.
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Immunology
    • 测定原理:
      quantitative
    • 测定方法:
      Sandwich
    • 说明书:
    • 数据处理:
    • 货期:
      3-5 working days

    引用文献

    产品评价

    问答及客户评论

     客户评论
    • Tested application: ELISA
      Test sample: Tissue homogenate (brain)
      Species: Rat
      Review: The test is carried out in a simple way, the samples in repetitions come out very repetitive, only small differences in groups. The samples were not diluted, the results approximated to half the standard curve. The samples were converted to the amount of protein calculated using the BCA method.

    靶点详情

    • 功能:
      Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
    • 亚细胞定位:
      Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
    • 蛋白家族:
      IL-1 family
    • 数据库链接:


    1v1周青梅郑宣木天寥
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