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    4. Rat Cortisol ELISA Kit

    Rat Cortisol ELISA Kit

    • 中文名称：
      大鼠皮质醇(Cortisol)ELISA Kit
    • 货号：
      CSB-E05112r
    • 规格：
      96T/48T
    • 价格：
      ￥3900/￥2500
    • 其他：

    产品详情

    • 产品描述：

      This Rat COR ELISA Kit was designed for the quantitative measurement of Rat COR protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.049 ng/mL-200 ng/mL and the sensitivity is 0.049 ng/mL.

    • 缩写：
      COR
    • 种属：
      Rattus norvegicus (Rat)
    • 样本类型：
      serum, plasma
    • 检测范围：
      0.049 ng/mL-200 ng/mL
    • 灵敏度：
      0.049 ng/mL
    • 反应时间：
      1-5h
    • 样本体积：
      50-100ul
    • 检测波长：
      450 nm
    • 研究领域：
      Metabolism
    • 测定原理：
      quantitative
    • 测定方法：
      Sandwich
    • 说明书：
      下载最新说明书
    • 精密度：
      Intra-assay Precision (Precision within an assay): CV%<8%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<10%      
      Three samples of known concentration were tested in twenty assays to assess.    
                   
    • 线性度：
      To assess the linearity of the assay, samples were spiked with high concentrations of rat cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      　 Sample Serum(n=4)  
      1:1 Average % 90  
      Range % 86-98  
      1:2 Average % 96  
      Range % 92-104  
      1:4 Average % 85  
      Range % 81-88  
      1:8 Average % 90  
      Range % 83-97  
    • 回收率：
      The recovery of rat cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 91 87-94  
      EDTA plasma (n=4) 96 92-100  
                   
                   
    • 标准曲线：
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      ng/ml OD1 OD2 Average    
      200 0.195 0.185 0.190    
      50 0.336 0.321 0.329    
      12.5 0.515 0.553 0.534    
      3.12 0.913 0.982 0.948    
      0.78 1.334 1.261 1.298    
      0.195 1.663 1.656 1.660    
      0.049 1.933 1.900 1.917    
      0 2.326 2.402 2.364    
    • 数据处理：
      ELISA操作视频及标曲绘制软件下载
    • 货期：
      3-5 working days

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    问答及客户评论

    ■ 常见问题解答
    Q:

    Can we use processed brain tissue samples of rat for analysis?/

    A:
    Thanks for your inquiry.
    We suggest you do a pretest first if you want to test brain tissues. Pls grind well when treating the samples.
    Pls let me know if you have any further questions. Thank you.
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