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  • Your Good Partner in Biology Research

    Pig Angiopoietin 1,ANG-1 ELISA Kit

    • 中文名称:
      猪血管生成素1(ANG-1)ELISA Kit
    • 货号:
      CSB-E08457p
    • 规格:
      96T/48T
    • 价格:
      ¥4200/¥3000
    • 其他:

    产品详情

    • 别名:
      ANGPT1Angiopoietin-1 ELISA kit; ANG-1 ELISA kit
    • 缩写:
      ANGPT1
    • Uniprot No.:
    • 种属:
      Sus scrofa (Pig)
    • 样本类型:
      serum, plasma, tissue homogenates
    • 检测范围:
      6 ng/mL-200 ng/mL
    • 灵敏度:
      6 ng/mL
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Cardiovascular
    • 说明书:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<15%        
      Three samples of known concentration were tested twenty times on one plate to assess.    
      Inter-assay Precision (Precision between assays): CV%<15%        
      Three samples of known concentration were tested in twenty assays to assess.      
                     
    • 线性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
        Sample Serum(n=4)    
      1:1 Average % 95    
      Range % 90-105    
      1:2 Average % 95    
      Range % 85-100    
      1:4 Average % 89    
      Range % 85-101    
      1:8 Average % 90    
      Range % 80-99    
    • 回收率:
      The recovery of pig ANG-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
       
      Sample Type Average % Recovery Range    
      Serum (n=5) 97 92-105    
      EDTA plasma (n=4) 96 90-102    
                     
                     
    • 标准曲线:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
       
      ng/ml OD1 OD2 Average      
      200 0.345 0.351 0.348      
      100 0.478 0.486 0.482      
      50 0.703 0.716 0.710      
      17 1.068 1.103 1.086      
      6 1.316 1.355 1.336      
      0 1.925 1.968 1.947      
    • 货期:
      3-5 working days

    产品评价

    靶点详情

    • 功能:
      Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
    • 基因功能参考文献:
      1. Angiopoietin 1 was predominantly present in the maturing glomeruli. PMID: 20598355
      2. The expression of ANGPT1 has already been studied during metanephric glomerulogenesis, but it remains to be elucidated during the development of the embryonic mesonephros. PMID: 20713984
      3. Attenuates H2O2-induced stress-activated protein kinase (SEK)1/JNK phosphorylation through the PI 3-kinase/Akt pathway and inhibits the apoptosis of vascular endothelial cells induced by oxidative stress. PMID: 16000309
      4. Estrogen-dependent control of Ang-1 expression plays an important role in stabilizing meningeal microvessel and maintaining healthy microvascular networks. PMID: 17496211
    • 亚细胞定位:
      Secreted.
    • 数据库链接:


    1v1周青梅郑宣木天寥
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