Mouse angiotensin Ⅱ(ANG-Ⅱ) ELISA Kit
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中文名称:小鼠血管紧张素Ⅱ(ANG-Ⅱ)ELISA Kit
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货号:CSB-E04495m
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规格:96T/48T
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价格:¥3200/¥2500
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其他:
产品详情
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产品描述:
This Mouse ANG-Ⅱ ELISA Kit was designed for the quantitative measurement of Mouse ANG-Ⅱ protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 pg/mL-30 pg/mL and the sensitivity is 0.11 pg/mL .
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别名:angiotensin Ⅱ, angiotensin 2, ANG2
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缩写:ANG-Ⅱ
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种属:Mus musculus (Mouse)
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样本类型:serum, plasma, cell culture supernates, tissue homogenates
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检测范围:0.45 pg/mL-30 pg/mL
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灵敏度:0.11 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cardiovascular
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测定原理:quantitative
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测定方法:Sandwich
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说明书:
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 90-95 1:2 Average % 102 Range % 98-104 1:4 Average % 92 Range % 89-96 1:8 Average % 100 Range % 95-103 -
回收率:
The recovery of mouse ANG-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 90-95 EDTA plasma (n=4) 96 92-99 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 30 2.166 2.072 2.119 1.980 15 1.434 1.422 1.428 1.289 7.5 0.985 0.971 0.978 0.839 3.75 0.489 0.497 0.493 0.354 1.8 0.313 0.324 0.319 0.180 0.9 0.238 0.242 0.240 0.101 0.45 0.187 0.181 0.184 0.045 0 0.140 0.138 0.139 -
数据处理:
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货期:3-5 working days
引用文献
- Bone morphogenetic protein 4 in perivascular adipose tissue ameliorates hypertension through regulation of angiotensinogen WJ Mu,Frontiers in cardiovascular medicine,2022
- Imatinib and methazolamide ameliorate COVID-19-induced metabolic complications via elevating ACE2 enzymatic activity and inhibiting viral entry Zilun L,Cell Metabolism,2022
- PSGL1-deficient mice develop spontaneous pulmonary hypertension associated to systemic sclerosis Gonzalez-Tajuelo R, et al,Arthritis & Rheumatology,2019
- The combination effects of Shen-Ling-Bai-Zhu on promoting apoptosis of transplanted H22 hepatocellular carcinoma in mice receiving chemotherapy. Xi S.et al,J Ethnopharmacol.,2016
- The effects of different angiotensin II type 1 receptor blockers on the regulation of the ACE-AngII-AT1 and ACE2-Ang(1-7)-Mas axes in pressure overload-induced cardiac remodeling in male mice. Wang X.et al,J Mol Cell Cardiol.,2016
- Pitavastatin-attenuated cardiac dysfunction in mice with dilated cardiomyopathy via regulation of myocardial calcium handling proteins WEI HU et al,Acta pharmaceutica,2014
- Control of Adipogenesis by the Autocrine Interplays between Angiotensin (1-7)/Mas receptor and Angiotensin II/AT1 receptor Signaling Pathways Than A et al,J Biol Chem,2013
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