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    Mouse Transforming Growth factor β1,TGF-β1 ELISA kit

    • 中文名称:
      小鼠转化生长因子β1(TGF-β1)ELISA Kit
    • 货号:
      CSB-E04726m
    • 规格:
      96T/48T
    • 价格:
      ¥3800/¥2500
    • 其他:

    产品详情

    • 产品描述:

      This Mouse TGFB1 ELISA Kit was designed for the quantitative measurement of Mouse TGFB1 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.2 ng/mL.

    • 别名:
      Tgfb1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
    • 缩写:
      TGFB1
    • Uniprot No.:
    • 种属:
      Array
    • 样本类型:
      serum, plasma, cell culture supernates
    • 检测范围:
      0.78 ng/mL-50 ng/mL
    • 灵敏度:
      0.2 ng/mL
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Signal Transduction
    • 测定原理:
      quantitative
    • 测定方法:
      Sandwich
    • 说明书:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<10%      
      Three samples of known concentration were tested in twenty assays to assess.    
                   
    • 线性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
        Sample Serum(n=4)  
      1:1 Average % 99  
      Range % 94-104  
      1:2 Average % 89  
      Range % 83-97  
      1:4 Average % 92  
      Range % 87-97  
      1:8 Average % 92  
      Range % 87-96  
    • 回收率:
      The recovery of mouse TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 92 87-99  
      EDTA plasma (n=4) 96 90-100  
                   
                   
    • 标准曲线:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      ng/ml OD1 OD2 Average Corrected  
      50 2.654 2.636 2.645 2.515  
      25 2.318 2.455 2.387 2.257  
      12.5 1.756 1.886 1.821 1.691  
      6.25 0.997 1.001 0.999 0.869  
      3.12 0.542 0.512 0.527 0.397  
      1.56 0.301 0.309 0.305 0.175  
      0.78 0.228 0.238 0.233 0.103  
      0 0.129 0.131 0.130    
    • 本试剂盒所含材料:
      • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse TGF-β1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-labeled TGF-β1 antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
      • HRP-avidin Diluent 1 x 15 ml---Dilute the high concentration HRP-avidin solution to an appropriate solution.
      • Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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    • 本试剂盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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    • 数据处理:
    • 货期:
      3-5 working days

    引用文献

    产品评价

    平均分:
    4.0分 - 1 个评价

    样品类型:血清

    样品信息:小鼠

    稀释比:没有稀释

    产品评价: 我用CSB-E04726m分别检测了细胞上清外泌体和小鼠上清的tgfβ水平。标曲结果良好。

    By 匿名

    靶点详情

    • 功能:
      Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.; Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1. Interaction with LRRC33/NRROS regulates activation of TGF-beta-1 in macrophages and microglia. Interaction with LRRC32/GARP controls activation of TGF-beta-1 on the surface of activated regulatory T-cells (Tregs). Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1.; Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1. Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules'. Plays an important role in bone remodeling: acts as a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types.
    • 基因功能参考文献:
      1. this paper shows that epithelial-derived TGF-beta1 acts as a pro-viral factor in the lung during influenza A infection PMID: 29067998
      2. Study points toward elevated levels of active TGF-beta as inducers and promoters of ectopic bone formation, and suggest that TGF-beta might be a therapeutic target in heterotopic ossification. PMID: 29416028
      3. The comparison of transforming growth factor beta family (TGFbeta) expression showed significantly higher levels of Tgfbeta3 transcript between nude and Balb/c mice but no differences were detected for Tgfbeta1. Nude DFs were specifically sensitive to the presence of the pro-regenerative TGFbeta3 isoform, showing increased collagen I deposition and alpha smooth muscle actin expression. PMID: 29637306
      4. Results indicate that the miR-23a cluster regulates osteocyte differentiation by modulating the TGF-beta signalling pathway. PMID: 28397831
      5. Following Schistosoma exposure, TSP-1 levels in the lung increase, via recruitment of circulating monocytes, while TSP-1 inhibition or knockout bone marrow prevents TGF-beta activation and protects against pulmonary hypertension development. PMID: 28555642
      6. TGF-beta/Smad proteins signaling affects radiation response and prolongs survival by regulating DNA repair genes in malignant glioma. PMID: 30230914
      7. The results indicate that EGFR and its activation are critical for YAP-mediated suppression of TGF-beta1-induced apoptosis. This study provides a new understanding of the regulatory mechanism underlying the determination of cell fate in response to TGF-beta1-mediated simultaneous apoptosis and epithelial mesenchymal transformation. PMID: 28425446
      8. transforming growth factor beta (TGFbeta) signaling was upregulated in HSCs from bone marrow of mice with MLL-AF9-induced acute myeloid leukemia (AML) because of excessive production of TGFbeta1, especially from megakaryocytes, and overactivation of latent TGFbeta1 protein. PMID: 29307605
      9. TGF-beta release from platelets is necessary for podoplanin-mediated tumor invasion and metastasis in lung cancer. PMID: 28176852
      10. Data suggest partial or complete transforming growth factor beta 1 (TGFBI) knockdown as a potential therapy against TGFBI-linked corneal dystrophies. PMID: 29117645
      11. De novo formation of the biliary system by TGFbeta-mediated hepatocyte transdifferentiation PMID: 29720662
      12. CXCL9 may promote prostate cancer progression via inhibition of cytokines from T cells. PMID: 29901197
      13. EGCG attenuated airway inflammation in asthmatic mice, decreased the percentage of Th17 cells and increased the percentage of Treg cells. The antiinflammatory effect of EGCG is achieved via the TGFbeta1 signaling pathway. PMID: 29916550
      14. fibrosis induced by Ang II may be alleviated by AKAP12 expression through inactivation of the TGF-beta1 pathway. PMID: 29501491
      15. RUNX1 promoted TGF-beta-induced partial EMT by increasing transcription of the PI3K subunit p110delta, which mediated Akt activation. PMID: 29759484
      16. Transforming growth factor-beta1 induces cerebrovascular dysfunction and astrogliosis through angiotensin II type 1 receptor-mediated signaling pathways PMID: 29505736
      17. TET2 plays an important role in the pathogenesis of diabetic nephropathy by activating TGFbeta1 expression through demethylation of CpG islands in the TGFbeta1 regulatory region PMID: 29705354
      18. early myocardial CTGF mRNA expression (six hours) after Ang-II exposure is likely dependent on latent TGF-beta activation via the canonical Smad-dependent pathway in resident cardiac cells. PMID: 29575960
      19. These results indicate that the hepatocytic expression of TGF-beta and CTGF is mediated by Wnt signalling in Schistosoma japonicum infection. PMID: 28331224
      20. Infection with Mycobacterium bovis results in increase in interleukin-1alpha, TGF-beta1, and MMP1 in multinucleated macrophages. PMID: 29504104
      21. type I and II IFN as in vivo modifiers of LIC fate that may significantly affect the impact of putative leukemia-driving activities such as the ETV6-RUNX1-mediated growth advantage in the presence of TGF-beta and TLR4-upregulated mutagenic activity PMID: 27220664
      22. Studies indicate key developmental processes that require transforming growth factor beta (TGF-beta) signaling [Review]. PMID: 29190317
      23. Osthole,a natural coumarin, decreases the collagen/III ratio in transforming growth factor beta 1 (TGF-beta1)-transfected mouse cardiac fibroblasts via the TGF-beta/Smad signaling pathway. PMID: 29860992
      24. both ERK and Smad2 signal pathways are involved in the activation of macrophages induced by TGF-b1 and high-ambient glucose, while there is no crosstalk shown in the process. PMID: 29199516
      25. In obese mice, periodontitis caused the downregulation of MMP2, and upregulation of TIMP1 and TGF-beta1 at transcriptional and translational levels PMID: 29322806
      26. The protective effect of the EP2 receptor on TGF-beta1 induced podocyte injury via the PI3K / Akt signaling pathway. PMID: 29746568
      27. This study demonstrates that prevention of renal apoB accumulation is a mechanism by which TGF-beta inhibition is nephroprotective. PMID: 28912302
      28. data show that increased TGFbeta in the tumour microenvironment represents a primary mechanism of immune evasion that promotes T-cell exclusion and blocks acquisition of the TH1-effector phenotype; immunotherapies directed against TGFbeta signalling may therefore have broad applications in treating patients with advanced colorectal cancer PMID: 29443964
      29. IL 6 and TGF beta perform essential role in cerebral malaria pathogenesis by modulating the level of glial cell induced neuroinflammation. PMID: 28803696
      30. The increased susceptibility to IMQ-induced psoriasis of GILZ-Tg mice was significantly associated with skin-specific over-activation of TGF-beta1-mediated signaling via SMAD2/3. PMID: 27934944
      31. The data suggest that B cells can down-regulate the function of antigen-presenting cells, and in turn encephalitogenic Th1/Th17 responses, via TGF-beta1. PMID: 27708418
      32. p-SMAD2/3 and p-ERK1/2 might play a regulatory role in TGF-beta1 induced CTGF exp p-SMAD2/3 and p-ERK1/2 might play a regulatory role in TGF-beta1 induced CTGF expression during tooth development. PMID: 28825193
      33. inhibiting NCAM1 would be cardioprotective, counteract the pathological action of TGFbeta1 and reduce heart failure severity. PMID: 28870505
      34. TGF-beta signaling has a role in inhibiting tumor progression and invasion in an induced mouse bladder cancer model PMID: 27378170
      35. calpains inhibition plays crucial roles in vascular restenosis by preventing neointimal hyperplasia at the early stage via suppression of the MMP2/TGF-beta1 pathway. PMID: 27453531
      36. Suggest an essential role for platelet-derived TGFbeta1 for the vascular remodelling response to arterial injury, apparently independent from the role of platelets in thrombosis or haemostasis. PMID: 28726976
      37. In cultured B16 melanoma and Bend3 endothelial cells treated with Bend3 conditioned media, MITF, tyrosinase, and melanin expression decreased due to TGFB1 secreted by the endothelial cells. PMID: 27172887
      38. Lycat regulates TGF-beta mediated lung fibroblast differentiation in pulmonary fibrosis. PMID: 28751023
      39. TFGbeta1 and arachidonic acid synthase expression is increased during the involution period in mammary glands. PMID: 28381667
      40. Chronic exercise suppressed the TGF-beta1/IkB-alpha axis in the hypothalamus and improved the energy homeostasis in an animal model of obesity-associated with aging. PMID: 28854149
      41. miR-146 inhibits skeletal muscle fibrosis by suppressing TGF-beta/Smad4 signaling pathway. PMID: 28510617
      42. Study shows that expression of Gadd45 family members is adjusted in response to KCl/depolarization, BDNF/TRKB, and/or TGFB-signaling. Additionally, results indicate decreased Gadd45 expression in a mouse model of depression, and that Gadd45b expression and DNA demethylation activate Arc transcription. PMID: 28444170
      43. Bioinformatics analysis provides positive supportive correlation between somatic mutations for VD-related genes and the TGF-beta pathway PMID: 27456065
      44. Taken together, these results indicate that eIF6 may be involved in external mechanical force-mediated murine dermal fibroblast function at least partly through the TGF-beta1/TGFBR1/TGFBR2 pathway. PMID: 27824055
      45. important roles in virtually every stage of neural development [review] PMID: 28130363
      46. These data suggest that the interplay between cell-matrix adhesion and intercellular adhesion is an important determinant for some aspects of TGFbeta1-induced epithelial-mesenchymal transition via alphaSMA expression induction. PMID: 27194451
      47. Excessive activation of TGFbeta by spinal instability causes vertebral endplate sclerosis and intervertebral disk degeneration. PMID: 27256073
      48. A Smad3-PTEN regulatory loop controls proliferation and apoptotic responses to TGF-beta in mouse endometrium. PMID: 28524854
      49. TGFss through the Alk1/TgfssR2 receptor axis is acting on endothelial cells to produce hematopoiesis. PMID: 29253505
      50. The adoptive transfer of NK1.1(-) CD4(+) NKG2D(+) cells suppressed DSS-induced colitis largely dependent on TGF-beta. Thus, NK1.1(-) CD4(+) NKG2D(+) cells exhibited immune regulatory functions, and this T cell subset could be developed to suppress inflammation in clinics. PMID: 28224733

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    • 亚细胞定位:
      [Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-1]: Secreted.
    • 蛋白家族:
      TGF-beta family
    • 数据库链接:


    1v1周青梅郑宣木天寥
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