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    Mouse Interleukin 1α,IL-1α ELISA kit

    • 中文名称:
      小鼠白介素1α(IL-1α)ELISA Kit
    • 货号:
      CSB-E04621m
    • 规格:
      96T/48T
    • 价格:
      ¥3200/¥2500
    • 其他:

    产品详情

    • 产品描述:

      This Mouse IL1A ELISA Kit was designed for the quantitative measurement of Mouse IL1A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 2.35 pg/ml - 150 pg/ml and the sensitivity is 0.58 pg/ml.

    • 别名:
      Il1aInterleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
    • 缩写:
      IL1A
    • Uniprot No.:
    • 种属:
      Mus musculus (Mouse)
    • 样本类型:
      serum, plasma, tissue homogenates
    • 检测范围:
      2.35 pg/ml - 150 pg/ml
    • 灵敏度:
      0.58 pg/ml
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Immunology
    • 测定原理:
      quantitative
    • 测定方法:
      Sandwich
    • 说明书:
    • 精密度:

        

    • 线性度:

       
       
        

    • 回收率:

        

    • 标准曲线:

         

    • 本试剂盒所含材料:
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse IL-1α antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled IL-1α antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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    • 本试剂盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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    • 数据处理:
    • 货期:
      3-5 working days

    产品评价

    靶点详情

    • 功能:
      Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
    • 基因功能参考文献:
      1. Infection with Mycobacterium bovis results in increase in interleukin-1alpha, TGF-beta1, and MMP1 in multinucleated macrophages. PMID: 29504104
      2. Together, these data suggest that caspase-11/IL-1alpha pathway plays an important role in defending against Klebsiella pneumoniae by recruiting neutrophils in the early stage of infection. PMID: 28939441
      3. These data highlight an important interdependency between the potent pro-inflammatory cytokine IL1A and Fshr expression. PMID: 28337831
      4. Since neither IL-1alpha nor IL-1beta depletions completely rescued the phenotype, we believe that IL-1alpha and IL-1beta have a similar and probably complementary role in FHF progression PMID: 28953903
      5. These results suggested that Streptococcus pneuomoniae PLY induces the influx of calcium in Streptococcus pneumoniae-infected macrophages, followed by calpain activation and subsequent IL-1alpha maturation and secretion. PMID: 28630064
      6. In response to chemically induced colitis, this microbial landscape promoted the release of IL-1alpha, which acted as a critical driver of colitis and colitis-associated cancer. PMID: 27775548
      7. our results suggest that mature IL-1alpha induced by hS100A7 is via RAGE-p38 MAPK and calpain-1 pathway in keratinocyte and this mechanism may play an important role during psoriasis. PMID: 28060905
      8. Il-1 signaling pathway has a key role in abdominal aortic aneurysm formation in mouse model of Kawasaki disease. PMID: 26941015
      9. endothelial cells were identified as the primary cellular source of G-CSF during OPC, which responded to IL-1alpha that was released from keratinocytes in the infected tissue. PMID: 27632536
      10. Key aspects of IL-1alpha biology and regulation especially with regard to inflammation are reviewed. Review. PMID: 27434011
      11. data suggested that pINSd needs IL-1R1 for inflammatory cytokine induction. Mouse embryo fibroblast cells of IL-1R1-deficient mice further confirmed that pINSd promotes immune responses through IL-1R1 PMID: 27226621
      12. IL-1alpha signaling and DNA damage is important for triggering a sterile inflammatory cascade . PMID: 26439902
      13. As a dual function cytokine, IL-1alpha may contribute to the induction of CHOP intracellularly, while IL-1alpha released from necrotic cells accelerates steatohepatitis via induction of inflammatory cytokines by neighboring cells. PMID: 26022690
      14. These data demonstrate that DC and macrophages display distinct patterns of cytokine regulation, particularly with respect to IL-1, as a consequence of cell-type specific differences in the physicochemical properties of the P2X(7)R PMID: 26068648
      15. Data suggest the role of stromal cell IL-1alpha and IL-1beta in Kawasaki disease vasculitis model. PMID: 26515418
      16. IL-1alpha and IL-36alpha form a self-amplifying inflammatory loop in vivo that in patients with insufficient counter regulatory mechanisms may become hyper-engaged and/or chronic PMID: 26203636
      17. IL-1alpha-positive cells were identified in the epithelium in dextran sulfate sodium (DSS)-induced colitis. IL-1alpha was detected in the stool of colitic mice before IL-1beta. PMID: 25864926
      18. IL-1alpha acts as an alarmin essential for leukocyte recruitment and protective immunity against HSV-1 PMID: 25323745
      19. The aim of this study was to characterize the role of IL-1 in cellular responses of carbon nanotubes in cells from IL-1alpha/beta wild type (IL1-WT) mice. PMID: 25748835
      20. These findings do not support the previously suggested role of nuclear IL-1alpha in gene regulation of IL-1beta. PMID: 25748836
      21. the underlying mechanism by which AR influences AAA development is through IL-1alpha and transforming growth factor-beta1, and provides a potential new therapy to suppress/prevent AAA by targeting AR with ASC-J9. PMID: 26324502
      22. inhibition of this potentially important source of chronic inflammation in atherosclerosis requires blockade of interleukin-1alpha and not interleukin-1beta. PMID: 26139463
      23. The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines. PMID: 25058342
      24. data suggest that central inhibition of IL-1alpha or Tox3 overexpression during the acute phase of a CNS insult may be an effective means for preventing the loss of neurological function PMID: 26224856
      25. Data (including date from studies in knockout mice) suggest that neutralization/deletion of Il1a reduces Il1b production and neutrophil infiltration in lung after inhalation exposure to silica nanoparticles. PMID: 25497724
      26. The controlled release of IL-1alpha could be a critical regulator during acute liver inflammation PMID: 25870999
      27. it appears the balance between TPO and IL-1alpha determines the MK cellular programming for thrombopoiesis in response to acute and chronic platelet needs. PMID: 25963822
      28. In acute lung injury, lipopolysaccharide induced alveolar macrophage necrosis via CD14 and the P2X7 receptor leading to interleukin-1alpha release. PMID: 25862090
      29. IL-1alpha induced the proliferation of CD11b(low) alveolar macrophages and differentiated these cells into CD11b(high) macrophages which perform critical phagocytic functions and organize granuloma. PMID: 25421226
      30. Data indicate that interleukin-1 cytokines IL-1alpha and IL-1beta are regulated by polyubiquitination and proteasomal degradation. PMID: 25371210
      31. IL-1 is a key mediator driving an innate immune response to inflammatory challenge in the mouse brain but is dispensable in extracerebral tissues including the lung and peritoneum. PMID: 25367678
      32. findings identify IL-1alpha as a crucial early danger signal triggering post-MI inflammation. PMID: 25505286
      33. Immune complexes inhibit IL-1 secretion and inflammasome activation. PMID: 25320279
      34. Selective deficiency of IL-1alpha in Kupffer cells reduces liver inflammation and expression of inflammatory cytokines, which may implicate Kupffer cell-derived IL-1alpha in steatohepatitis development. PMID: 24582082
      35. cigarette smoke-induced neutrophilia was dependent on IL-1alpha produced by alveolar macrophages and alveolar macrophages isolated from smoke-exposed mice were primed for excessive IL-1alpha production in response to bacterial ligands. PMID: 25092891
      36. tested the possible role of the proinflammatory cytokine IL-1 in the age-related exhaustion of ovarian reserve using IL-1alpha and IL-1beta-KO mice PMID: 25114230
      37. This study documents distinct roles for IL-1alpha and IL-1beta in the response to Pseudomonas aeruginosa infection as a function of the type 3 secretion system effectors produced by the infecting strain. PMID: 25069982
      38. necroptosis caused the processing and release of IL-1alpha, and this was independent of IL-1beta processing and release PMID: 24790078
      39. IL-1 induces systemic inflammation and augments Streptococcal pneumoniae infection, atherosclerosis, and ischemic brain injury via platelet activation and microvascular coagulation. PMID: 24644058
      40. IL-1alpha was not released upon inflammasome activation unless significant cell damage occurred. PMID: 23684408
      41. haematopoietic-derived IL-1 is a key driver of ischaemic brain injury. PMID: 23519030
      42. In severe S. aureus bacteraemia in mice, TNF-alpha, IL-1alpha, and KC are biomarkers predicting fatal outcome of infection. PMID: 23520553
      43. Our results establish IL-1alpha as a critical initiator of the inflammatory response to L. pneumophila PMID: 23686480
      44. discovery of a novel inflammatory circuit in which RIP1-mediated IL-1alpha secretion in response to deregulated SHP-1 activity triggers an inflammatory destructive disease that proceeds independently of inflammasomes and programmed necrosis PMID: 23708968
      45. Results suggest importance of IL-1R1/IL-1alpha to the recruitment and activation of dendritic cells in response to cigarette smoke exposure. PMID: 22992200
      46. ERalpha-regulated repression of TNFalpha and IL1alpha is important for lumen formation and maintenance. PMID: 22328525
      47. Allergic sensitization to HDM was abolished in vivo when IL-1alpha, GM-CSF, or IL-33 was neutralized. PMID: 22802353
      48. Autophagy has a potentially pivotal role to play in the induction and regulation of inflammatory responses by innate immune cells, largely driven by IL-1 and its consequential effects on IL-23 secretion. PMID: 22972933
      49. Vascular wall resident cells are the main targets for the pro-atherogenic effects of bone marrow-derived IL-1 through IL-1R1, partly by induction of adhesion and chemotactic molecules in endothelial cells. PMID: 22236482
      50. This study demonstrated here in in vivo experiments that IL-1 exacerbates the effects of SCI by accentuating the impact of the inflammatory responses. PMID: 22483094

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    • 亚细胞定位:
      Cytoplasm. Secreted.
    • 蛋白家族:
      IL-1 family
    • 数据库链接:


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