Mouse Corticosterone,CORT ELISA Kit

• A micro ELISA plate --- The 96-well plate has been pre-coated with goat anti-rabbit CORT antibody.
• Six vials lyophilized standard (0.5 ml/bottle) --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
• Anti-mouse CORT Antibody (1 x 6 ml) --- Act as the detection antibody.
• HRP-conjugate (1 x 6 ml) --- HRP catalyzes TMB substrate to elicit a chromogenic reaction.
• Wash Buffer (20 x concentrate) (1 x 15 ml) --- Wash away unbound or free substances.
• Substrate A (1 x 7 ml) --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
• Substrate B (1 x 7 ml) --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
• Stop Solution 1 x 7 ml --- Stop the color development, and the eventual solution color turns yellow.
• Four Adhesive Strips (For 96 wells) --- Cover the microwell plate when incubation.
• An Instruction manual

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• A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm to 630 nm.
• An incubator that can provide stable incubation conditions up to 37°C±5°C.
• Centrifuge
• Vortex
• Squirt bottle, manifold dispenser, or automated microplate washer
• Absorbent paper for blotting the microtiter plate
• 50-300ul multi-channel micropipette
• Pipette tips
• Single-channel micropipette with different ranges
• 100ml and 500ml graduated cylinders
• Deionized or distilled water
• Timer
• Test tubes for dilution

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