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  • Your Good Partner in Biology Research

    Mouse B-cell leukemia/lymphoma 2,Bcl-2 ELISA Kit

    • 中文名称:
      小鼠B细胞淋巴瘤因子2(Bcl-2)ELISA Kit
    • 货号:
    • 规格:
    • 价格:
    • 其他:


    • 产品描述:

      This Mouse BCL2 ELISA Kit was designed for the quantitative measurement of Mouse BCL2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

    • 别名:
      Bcl2 ELISA Kit; Bcl-2 ELISA Kit; Apoptosis regulator Bcl-2 ELISA Kit
    • 缩写:
    • Uniprot No.:
    • 种属:
      Mus musculus (Mouse)
    • 样本类型:
      serum, plasma
    • 检测范围:
      15.6 pg/mL-1000 pg/mL
    • 灵敏度:
      3.9 pg/mL
    • 反应时间:
    • 样本体积:
    • 检测波长:
      450 nm
    • 研究领域:
      Cell Biology
    • 测定原理:
    • 测定方法:
    • 说明书:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<10%      
      Three samples of known concentration were tested in twenty assays to assess.    
    • 线性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of mouse Bcl-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
        Sample Serum(n=4)  
      1:5 Average % 96  
      Range % 85-102  
      1:10 Average % 94  
      Range % 85-102  
      1:20 Average % 94  
      Range % 82-103  
      1:40 Average % 90  
      Range % 81-99  
    • 回收率:
      The recovery of mouse Bcl-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 94 85-100  
      EDTA plasma (n=4) 96 87-105  
    • 标准曲线:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      pg/ml OD1 OD2 Average Corrected  
      1000 1.952 2.052 2.002 1.838  
      500 1.527 1.591 1.559 1.395  
      250 1.051 1.112 1.082 0.918  
      125 0.628 0.592 0.610 0.446  
      62.5 0.435 0.457 0.446 0.282  
      31.2 0.293 0.304 0.299 0.135  
      15.6 0.215 0.206 0.211 0.047  
      0 0.166 0.162 0.164    
    • 数据处理:
    • 货期:
      3-5 working days




    • 功能:
      Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function. May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release.
    • 基因功能参考文献:
      1. data demonstrate that disruption of the beclin 1-BCL2 complex is an effective mechanism to increase autophagy, prevent premature ageing, improve healthspan and promote longevity in mammals PMID: 29849149
      2. Results support a link between age-related apoptosis in auditory cortex neurons and miR-34a/Bcl-2 signaling, which may serve as a potential mechanism of the expression of age-related hearing loss in the auditory cortex. PMID: 28817812
      3. MiR-146a inhibits proliferation and induces apoptosis in murine osteoblastic MC3T3-E1 by regulating Bcl2 PMID: 28975995
      4. Ru(II)/diphenylphosphine/pyridine-6-thiolate complexes induce S-180 cell apoptosis through intrinsic mitochondrial pathway involving inhibition of Bcl-2 and p53/Bax activation. PMID: 28795366
      5. The results suggest that SPK2 interacts with Bcl2 via its BH3 domain, thereby dissociating it from Beclin-1 and activating autophagy while protecting neurons against ischemic injury. PMID: 28682313
      6. The findings support a model in which survival is determined by quantitative participation of multiple anti-apoptotic proteins, BCL2, Mcl1, and BCL2A1, rather than by a single anti-apoptotic protein. PMID: 28362427
      7. Bcl-2 is strongly expressed in the inner hair cells and spiral ganglion neurons of young mice. In addition, moderate Bcl-2 expression is also detected in the outer hair cells and in the neurons of the auditory cortex. A significant reduction of Bcl-2 expression in the cochlea or auditory cortex is also associated with elevated hearing thresholds and hair cell loss during aging. PMID: 27925611
      8. Bcl2 is the autophagy-related target of miR-449a. PMID: 27351886
      9. anti-apoptotic molecules BclxL and Bcl-2 and the pro-apoptotic factors BAD and BID cooperate to promote migration of triple-negative breast cancer cells stimulated with cl-CD95L. PMID: 27367565
      10. Acute ethanol exposure induced autophagy-mediated heart toxicity and injury mainly through the ROS-JNK-Bcl-2 signaling pathway. PMID: 28369910
      11. Th17 cells are resistant to glucocorticoid-induced apoptosis and cytokine suppression, at least in part due to high levels of BCL-2. These findings support a role of Th17 cells in glucocorticoid-resistant inflammatory conditions such as certain endotypes of asthma. PMID: 26752231
      12. E2 protects skeletal myoblasts against apoptosis induced by H2 O2 modulating p53 and FoxO transcription factors and then their target genes Bcl-2, Bim, Puma, PERP, and MDM2, without affecting Noxa gene. PMID: 27249370
      13. Bcl-2 expression has a significant impact on the mineralogical content of enamel crystals of tooth structure PMID: 28610838
      14. The results suggest that CARP can protect against hypoxia-reperfusion induced cardiomyocyte apoptosis, possibly through increasing anti-apoptosis Bcl2 gene expression. PMID: 27713078
      15. This study provides evidence from transgenic mouse models that Crebbp deletion results in deficits in B-cell development and can cooperate with Bcl2 overexpression to promote B-cell lymphoma. PMID: 28288979
      16. an essential role for the binding of BCL2 to BIM in the survival of noncycling NK cells. PMID: 28057804
      17. LIGHT signalling pathway combined with IFN-gamma induces beta cells apoptosis via an NF-kappaB/Bcl2-dependent mitochondrial pathway. PMID: 27241100
      18. Bcl-2 was highly expressed in tubulointerstitial infiltrates in (NZB x NZW)F1 (NZB/NZW) lupus-prone mice. PMID: 27159593
      19. Bcell lymphoma 2, an antiapoptotic gene, was the target of mmumiR96. PMID: 28259902
      20. The abnormal expression of epidermal cytokeratins suggests that Ha-Ras and Bcl-2 suppress the terminal differentiation and sustain the stem cell-like features in epidermal keratinocytes PMID: 27655119
      21. the augmentation of endogenous MTA1 expression during neuronal ischemic injury acts additionally to an endocrinous cascade orchestrating intimate interactions between ERalpha and BCL2 pathways. PMID: 26728277
      22. Interestingly, SKPs showed higher protein levels of Bcl-2, Nrf2, and HO-1 than FBs. SKPs exert a beneficial effect on resisting UVB-induced apoptosis, which may be associated with Bcl-2 and Nrf2 upregulation. PMID: 27635399
      23. Bcl-2 is an essential regulator in the survival of doublecortin-expressing immature neurons. PMID: 26266948
      24. Results show that the deletion of BCL-2, on its own or in concert with MCL-1, does not affect platelet production or platelet lifespan. PMID: 25880088
      25. the expression changes observed within the BCL2 family did not depend on STAT3 signalling, in line with its initiating role early in the process, but rather appear to result from relief of repression by STAT5. PMID: 26045049
      26. Bcl-2-caspase-9 apoptosis pathway was clearly activated in the testis of asthmatic mice with the increased expression of apoptosis-related proteins. PMID: 26938720
      27. Bcl-2 expression in the endothelium plays a significant role during postnatal retinal vascularization, and pathological choroidal but not retinal neovascularization, suggesting vascular bed specific Bcl-2 function in the endothelium PMID: 26444547
      28. Studied the protein expression of BCL2, FGF-R1, and HSP70 after short-time magnetic thermoablative tumor treatment using immunohistochemistry in a human BT474 breast cancer mouse xenograft model. PMID: 25792827
      29. The increased level of Bcl-2 caused by the decrease in miR-181c protected mitochondrial morphology from the tumour necrosis factor alpha-induced apoptosis. PMID: 25898913
      30. The expression of GRP78 and Bcl-2 was significantly higher in astrocytes pretreated with recombinant Shh. These findings suggest that the expression of Shh may inhibit cell death by activating Bcl-2 through a GRP78-dependent pathway. PMID: 25961032
      31. Knockdown of NF-kappaB p65 subunit expression can significantly inhibit the growth of nude mouse Lewis tumour cell xenografts by inducing tumour cell apoptosis and downregulating pro-apoptotic protein Bcl-2 expression. PMID: 26178579
      32. Studies suggest targeting the BCL-2 family for treating diseases with dysregulated apoptosis. PMID: 26344567
      33. Deletion of AU-rich elements within the Bcl2 3'UTR reduces protein expression and B cell survival PMID: 25680182
      34. The nuclear-penetrating anti-dsDNA autoantibodies could possibly function as a pathogenic factor for lupus nephritis by up-regulating ERK activation and Bcl-2 production in mesangial cells. PMID: 26189065
      35. Enhanced splicing of XBP-1 was observed in BCL-2 overexpressing cells implicating BCL-2 in the complex regulation of IRE1alpha activity. PMID: 26319553
      36. Bcl-2 counteracts palmitate-induced beta-cell death by maintaining mitochondrial membrane integrity and augmenting NF-kappaB activity, but not by affecting ROS production and ER stress PMID: 25266628
      37. BCL2-overexpressing B cells required multiple GC transits before acquiring FL-associated developmental arrest and presenting as GC B cells with constitutive activation-induced cytidine deaminase (AID) mutator activity. PMID: 25384217
      38. ZFP36L1 interacts with and mediates degradation of anti-apoptotic BCL2 mRNA as its important target in malignant B-cells. PMID: 25014217
      39. MPT64 could inhibit the apoptosis of RAW264.7 macrophages through the NF-kappaB-miRNA21-Bcl-2 pathway PMID: 25000291
      40. Suggest that when encountering harmful stress (hypoxia), melanoma cells overexpress Bcl-2 and turn to senescence, a permanent cell-cycle arrest, for prolonged survival. PMID: 24966955
      41. Bcl2 deficiency activates FoxO1 and FoxO3a through Akt inactivation and causes an increase in bone mass and osteoblast apoptosis. PMID: 24466179
      42. we demonstrate that inhibition of Bcl-2 increasingly stimulates NSCs proliferation, so that, it suggests that Bcl-2 controls NSCs differentiation to neurons. PMID: 25470948
      43. sigmaR1 regulates endoplasmic reticulum stress in retinal Muller cells and that the role of sigmaR1 in retinal neuroprotection probably involves BCL2. PMID: 24469320
      44. But not the IL-15 effect on Bcl-2 proteins. PMID: 24825007
      45. Data indicate that triggering receptor expressed on myeloid cells 1 (TREM-1) induces anti-apoptotic protein Bcl-2 and prolongs macrophage survival. PMID: 24711453
      46. Data suggest, in pancreatic beta-cells exposed to nonesterified fatty acid/palmitate, microRNA 34a expression is upregulated, apoptosis is promoted, and expression of Bcl2 is downregulated; silencing of Bcl2 gene expression promotes apoptosis. PMID: 24829923
      47. Differences in cell-death susceptibility between the undifferentiated and differentiated states showed that the mitochondria-localised anti-apoptotic protein Bcl-2 was highly expressed in undifferentiated Embryonic stem cells PMID: 24293136
      48. Bcl-2 levels are upregulated following traumatic brain injury, contributing to neuronal cell death. PMID: 25057207
      49. Myotubes of differentiated C2C12 cells with low levels of Bcl-2 expression are particularly vulnerable to apoptosis. PMID: 24129924
      50. In resistant Bcl2-expressing mouse lymphoma cells, 2 missense mutations within the Bcl2 BH3 domain were identified. Both F101C and F101L mutations impeded ABT-199 binding to the BH3 domain, therefore suppressing mitochondrial apoptosis PMID: 24786774



    • 亚细胞定位:
      Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
    • 蛋白家族:
      Bcl-2 family
    • 组织特异性:
      Expressed in a variety of tissues.
    • 数据库链接:

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