Mouse 4-Hydroxynonenal (HNE)ELISA Kit
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中文名称：小鼠4-羟基壬烯酸(HNE) ELISA Kit
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货号：CSB-E13412m
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：
This Mouse HNE ELISA Kit was designed for the quantitative measurement of Mouse HNE protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pmol/mL-1000 pmol/mL and the sensitivity is 3.9 pmol/mL.
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缩写：HNE
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种属：Mus musculus (Mouse)
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样本类型：serum, plasma
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检测范围：15.6 pmol/mL-1000 pmol/mL
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灵敏度：3.9 pmol/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Signal Transduction
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测定原理：quantitative
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测定方法：Sandwich
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说明书：
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 87-98 1:2 Average % 96 Range % 91-103 1:4 Average % 94 Range % 90-99 1:8 Average % 94 Range % 88-98 -
回收率：
The recovery of mouse HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 89-97 EDTA plasma (n=4) 94 90-99 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pmol/ml OD1 OD2 Average Corrected 1000 2.608 2.555 2.582 2.392 500 1.939 1.897 1.918 1.728 250 1.227 1.212 1.220 1.030 125 0.821 0.759 0.790 0.600 62.5 0.468 0.450 0.459 0.269 31.2 0.331 0.328 0.330 0.140 15.6 0.278 0.271 0.275 0.085 0 0.192 0.188 0.190 -
数据处理：
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货期：3-5 working days
引用文献
- Impact of mitochondrial aldehyde dehydrogenase 2 on cognitive impairment in the AD model mouse Y Yang,Acta Biochimica et Biophysica Sinica,2021
- Green tea polyphenol (-)-epigallocatechin-3-gallate triggered hepatotoxicity in mice: responses of major antioxidant enzymes and the Nrf2 rescue pathway Wang D.et al,Toxicol Appl Pharmacol.,2015
- Short-term treadmill exercise increased tau insolubility and neuroinflammation in tauopathy model mice Elahi M. et al,Neurosci Lett.,2016
- Region-Specific Vulnerability to Oxidative Stress, Neuroinflammation, and Tau Hyperphosphorylation in Experimental Diabetes Mellitus Mice. Elahi M.et al,J Alzheimers Dis.,2016
- Cancer Therapeutic Effects of Titanium Dioxide Nanoparticles Are Associated with Oxidative Stress and Cytokine Induction Fujiwara R. et al,Pathobiology.,2015
- Melatonin attenuates (-)-epigallocatehin-3-gallate triggered hepatotoxicity without compromising its downregulation of hepatic gluconeogenic and lipogenic genes in mice Wang D. et al,J Pineal Res,2015
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