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  • Your Good Partner in Biology Research

    Human inositol 1,4,5,-trisphosphate(IP3) ELISA kit

    • 中文名稱:
      人1,4,5-三磷酸肌醇(IP3)ELISA kit
    • 貨號:
      CSB-E12636h
    • 規格:
      96T/48T
    • 價格:
      ¥3600/¥2500
    • 其他:

    產品詳情

    • 產品描述:

      This Human IP3 ELISA Kit was designed for the quantitative measurement of Human IP3 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 5 pg/mL-1000 pg/mL and the sensitivity is 2.5 pg/mL.

    • 縮寫:
      IP3
    • 種屬:
      Homo sapiens (Human)
    • 樣本類型:
      serum, plasma, cell culture supernates, tissue homogenates
    • 檢測范圍:
      5 pg/mL-1000 pg/mL
    • 靈敏度:
      2.5 pg/mL
    • 反應時間:
      1-5h
    • 樣本體積:
      50-100ul
    • 檢測波長:
      450 nm
    • 研究領域:
      Signal Transduction
    • 測定原理:
      quantitative
    • 測定方法:
      Competitive
    • 說明書:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<15%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<15%      
      Three samples of known concentration were tested in twenty assays to assess.    
                   
    • 線性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of human IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
        Sample Serum(n=4)  
      1:1 Average % 95  
      Range % 90-105  
      1:2 Average % 94  
      Range % 89-100  
      1:4 Average % 94  
      Range % 87-102  
      1:8 Average % 95  
      Range % 90-102  
    • 回收率:
      The recovery of human IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 95 91-100  
      EDTA plasma (n=4) 92 87-96  
                   
                   
    • 標準曲線:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      pg/ml OD1 OD2 Average    
      1000 0.202 0.211 0.207    
      250 0.350 0.349 0.350    
      80 0.600 0.594 0.597    
      20 1.029 1.018 1.024    
      5 1.326 1.345 1.336    
      0 2.102 2.130 2.116    
    • 數據處理:
    • 貨期:
      3-5 working days

    引用文獻

    產品評價

    問答及客戶評論

     常見問題解答
    Q:

    I would like to receive more information about this assay, particularly regarding specificity for IP3.
    Please provide some more information about the following.
    a. capture antibody or capture antigen
    b. Detection antibody (if applicable)
    c. standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.
    Are you aware of any product specific references for this assay? If yes please can you provide links to these references. If you have any other information about validation of the antibodies in other applications e.g. WB this information would be helpful also.

    A:
    Thanks for your inquiry.
    CSB-E12636h
    This is a competitive kit.
    The plate is coated with goat-anti-rabbit antibody.
    The specific antibody is rabbit polyclonal antibody.
    The standard is purified IP3.
    The raw materials of the kit have been screened strictly and passed quality control. Sorry it is not convenient for us to provide validation information of the antibodies because it is only for our internal tracing. There are many references about this kit. Pls find following links:
    1. https://www.ncbi.nlm.nih.gov/pubmed/?term=26884398 https://academic.oup.com/humrep/article/31/3/672/2384908
    3. https://www.ncbi.nlm.nih.gov/pubmed/26181635
    4.https://www.ncbi.nlm.nih.gov/pubmed/25785721
    5. https://www.ncbi.nlm.nih.gov/pubmed/23992092
    6. https://www.nature.com/articles/nature10246?WT.ec_id=NATURE-20110728
    Pls let me know if you have any further questions. Thank you.


    1v1周青梅郑宣木天寥
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