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    Human high sensitivity C-Reactive Protein,hs-CRP ELISA Kit

    • 中文名称:
      人超敏C反应蛋白(hs-CRP)ELISA Kit
    • 货号:
      CSB-E08617h
    • 规格:
      96T/48T
    • 价格:
      ¥3600/¥2500
    • 其他:

    产品详情

    • 产品描述:

      This Human hs-CRP ELISA Kit was designed for the quantitative measurement of Human hs-CRP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

    • 别名:
      Pentraxin 1, short ELISA Kit; C reactive protein ELISA Kit; C reactive protein pentraxin related ELISA Kit; C-reactive protein(1-205) ELISA Kit; CRP ELISA Kit; CRP_HUMAN ELISA Kit; MGC88244 ELISA Kit; Pentraxin 1 ELISA Kit; PTX 1 ELISA Kit; PTX1 ELISA Kit
    • 缩写:
      hs-CRP
    • Uniprot No.:
    • 种属:
      Homo sapiens (Human)
    • 样本类型:
      serum, plasma, cell culture supernates, tissue homogenates
    • 检测范围:
      0.625 ng/mL-40 ng/mL
    • 灵敏度:
      0.156 ng/mL
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Others
    • 测定原理:
      quantitative
    • 测定方法:
      Sandwich
    • 说明书:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<10%      
      Three samples of known concentration were tested in twenty assays to assess.    
                   
    • 线性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of human CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
        Sample Serum(n=4)  
      1:200 Average % 95  
      Range % 91-99  
      1:400 Average % 104  
      Range % 100-108  
      1:800 Average % 93  
      Range % 89-97  
      1:1600 Average % 99  
      Range % 93-104  
    • 回收率:
      The recovery of human CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 92 87-96  
      EDTA plasma (n=4) 99 95-104  
                   
                   
    • 标准曲线:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      ng/ml OD1 OD2 Average Corrected  
      40 2.859 2.876 2.868 2.760  
      20 2.261 2.234 2.248 2.140  
      10 1.436 1.412 1.424 1.316  
      5 0.744 0.723 0.734 0.626  
      2.5 0.408 0.422 0.415 0.307  
      1.25 0.251 0.237 0.244 0.136  
      0.625 0.167 0.158 0.163 0.055  
      0 0.106 0.110 0.108    
    • 本试剂盒所含材料:
      • A micro ELISA plate--- The 96-well plate has been pre-coated with an anti-human hs-CRP antibody.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial HRP-conjugated hs-CRP antibody (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial HRP-avidin Diluent (20 ml/bottle) ---Dilute HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the standard and sample solutions.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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    • 本试剂盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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    • 数据处理:
    • 货期:
      3-5 working days

    引用文献

    产品评价

    靶点详情

    • 功能:
      Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
    • 基因功能参考文献:
      1. gemcabene decreases CRP by C/EBP-delta and NF-kappaB-mediated transcriptional mechanism and suppresses IL-6 and IL-1beta-induced CRP production. PMID: 29644527
      2. Combinative index (arterial lactate, NT-proBNP and CRP) could serve as a valuable predictor for the 28-day mortality rate in patients with sepsis. PMID: 30158031
      3. CRP rs3093061, rs1130864, rs1205, and rs3091244 polymorphisms are associated with decreased risk of developing Systemic Lupus erythematosus PMID: 29556849
      4. The study showed that higher CRP and lower T3 levels were associated with stroke severity on admission. Functional outcome is likely secondary to stroke severity but functional outcome at discharge was associated with higher CRP levels. PMID: 30056003
      5. These findings reveal that the CRP- and PTX3-binding characteristics of FHL-1 differ from those of FH, likely underpinning independent immune regulatory functions in the context of the human retina. PMID: 29374201
      6. Short-term changes in daily movement behaviour can affect salivary CRP, a marker of systemic inflammation. PMID: 29499122
      7. M1 macrophages were characterized by elevated CRP mRNA expression (by 67%), CRP protein levels (by 108%), and upregulation of NFkappaB activation PMID: 29687414
      8. Levels of CRP and PCT in CSF did not present greater accuracy in differentiating bacterial from viral meningitis compared to serum levels. PMID: 30143494
      9. elevated serum C-reactive protein (CRP) is a well-known proxy for infections/immune abnormalities. Our results show elevated C-reactive protein levels to be associated with an increase in risk for schizophrenia. PMID: 29306175
      10. High CRP expression is associated with Nonalcoholic fatty liver disease. PMID: 29402657
      11. High C-reactive protein expression is associated with impaired glucose tolerance. PMID: 29078256
      12. Findings indicate that childhood infections do not have an independent, lasting effect on circulating inflammatory marker levels subsequently in childhood; however, elevated inflammatory markers may be harmful for intellectual development/function. PMID: 29198208
      13. maternal serum level has predictive value for chorioamnionitis PMID: 29430983
      14. serum level elevated in preterm labor leading to preterm birth PMID: 29426269
      15. Among healthy women attempting pregnancy after one or two pregnancy losses, elevated C-reactive protein is associated with reduced fecundability. PMID: 29317123
      16. Suggest a close association between CRP and metabolic syndrome, while the association between the dietary inflammatory index and MetS was limited. PMID: 29954070
      17. pre-procedural serum CRP could be a valuable predictor of global cardiovascular risk. PMID: 28128312
      18. Data suggest that serum CRP levels show positive association with ChildTurcottePugh scores in subjects with liver cirrhosis; GSH levels show negative association with ChildTurcottePugh scores; thus, CRP and GSH appear to exert synergistic effects in association with severity of liver cirrhosis. This cross-sectional study was conducted in Taiwan. (GSH = glutathione) PMID: 29861471
      19. Study shows for the first time that CRP activates signaling of integrin alpha2 via binding to integrin alpha2 and Fcgamma receptor I in breast cells and MDA-MB-231 triple-negative human breast cancer cells. PMID: 28846105
      20. This meta-analysis suggests that C-reactive protein/albumin ratio (CAR)may be a potential prognostic marker in solid cancers PMID: 28128229
      21. The results of the study suggest that the levels of C-reactive protein and tumor necrosis factor-alpha are important diagnostic markers of inflammation in patients with chronic pancreatitis and type 2 diabetes mellitus. PMID: 30280549
      22. Elevated serum C-reactive protein could be a bacterial infection predictor in decompensated cirrhosis. PMID: 29384797
      23. The results showed that the MI-based bio-sensing system had high selectivity and sensitivity for detection of Cardiac biomarkers . Compared with the control region, ultrasensitive detections of CRP and Mb were accomplished with the detection limits of 1.0 pg/mL and 0.1 pg/mL, respectively. PMID: 29857573
      24. Data suggest that C-reactive protein (CRP) may play an important role in the development of tongue squamous cell carcinoma (TSCC). PMID: 29385406
      25. For WBC, N (%), and ESR, both groups showed similar patterns of temporal values 14 days after surgery regardless of preoperative CRP level. Our findings could be used as guidelines for patient discharge and during the follow-up period after surgery. PMID: 29854731
      26. The neutrophil/lymphocyte ratio is a better addition to C-reactive protein than CD64 index as a marker for infection in COPD. PMID: 28185443
      27. This retrospective cohort study aimed to identify the early postoperative kinetics of C-reactive protein (CRP) and procalcitonin (PCT) in children undergoing tetralogy of Fallot (ToF) correction. PMID: 29111402
      28. After beta-blocker treatment, hs-CRP levels are associated with functional capacity in heart failure patients. PMID: 29396658
      29. Hypertensive patients with coronary tortuosity have a higher serum hs-CRP level concentration and have a higher incidence of lacunar infarction than hypertensive patients without coronary tortuosity. PMID: 29436255
      30. High CRP expression is associated with non-small cell lung cancer. PMID: 29549910
      31. T allele of rs1205 in CRP gene was protective against Takayasu arteritis in our patients. CC genotype was associated with involvement of coronary artery and ascending aorta. A trend toward a blunted CRP response seen in patients. PMID: 29024426
      32. Serum C-reactive protein (and COMP) levels were both associated with the incidence of knee osteoarthritis. PMID: 29351749
      33. coexistence of both higher HCY and higher hs-CRP in the acute phase of ischemic stroke were associated with subsequent PSD, independently of established conventional risk factors PMID: 29325799
      34. Data suggest that C-reactive-protein (CRP) concentration is weakly associated with global DNA methylation level, and this association was more clearly in individuals with the minor allele of the 5,10-methylenetetrahydrofolate reductase (MTHFR)missense SNP rs1801133. PMID: 29439678
      35. A longitudinal association between adolescent CRP levels and adult schizophrenia diagnosis indicates a potentially important role of inflammation in the pathogenesis of the illness PMID: 27622678
      36. rs1205 genetic variability in the CRP gene determines the CRP levels in sepsis of different severities PMID: 29379005
      37. CRP levels predict the development of anastomotic leak and postoperative mortality after colorectal cancer surgery. PMID: 28132052
      38. Uremia patients receiving maintenance hemodialysis with hospitalacquired infection had increased serum inflammatory factors and high throughput hemodialysis significantly decreased CRP, IL2 and TNFalpha levels in the serum, suggesting that high throughput hemodialysis may be beneficial for the prevention of the infections in uremia patients. PMID: 29257244
      39. High CRP expression is associated with cardiovascular disease. PMID: 28724840
      40. The results of this meta-analysis suggest that increased CRP expression indicates a poorer prognosis in patients with bone neoplasms. PMID: 29668751
      41. C-reactive protein (CRP) recognized 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG) in the supported 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) monolayers without calcium at pH 7.4 and 5.5. PMID: 29172154
      42. Used Ultra Performance Liquid Chromatography-Ultra Definition Mass Spectrometry-based proteomics to compare serum samples from 19 colorectal cancer (CRC) patients. The samples were first analyzed after dividing them into two categories based on CRP, CRP <30 and CRP >30, and the same samples were analyzed again after being divided into the categories short and long 5-year survival. PMID: 29630649
      43. CRP bound to surface CD32 (also known as FcgammaRII) on myeloma cells, which activated a pathway mediated by the kinase p38 MAPK and the transcription factor Twist that enhanced the cells' secretion of osteolytic cytokines. PMID: 29233917
      44. high-sensitivity C-reactive protein could have a role in recurrent stroke and vascular events in patients treated with statins PMID: 28302952
      45. Procalcitonin and CRP serum levels are associated with the appearance of intra-abdominal infection after colorectal cancer surgery. PMID: 28918433
      46. C-reactive protein polymorphisms and DNA methylation of the AIM2 gene influence associations between trauma exposure, posttraumatic stress disorder, and C-reactive protein. PMID: 28867284
      47. Elevated CRP level is correlated with poor prognosis in patients with metastatic renal cell carcinoma receiving tyrosine kinase inhibitors treatment. (Meta-analysis) PMID: 29080691
      48. The study reveals that CRP might be a clear cell renal cell carcinoma development promoter. PMID: 28923830
      49. CRP distinguished sepsis and non-septic ICU patients. PMID: 28898742
      50. Suggest that hs-CRP may be a marker of comorbidities associated with worse health status in myocardial infarction. PMID: 28946036

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    • 亚细胞定位:
      Secreted.
    • 蛋白家族:
      Pentraxin family
    • 组织特异性:
      Found in plasma.
    • 数据库链接:

      HGNC: 2367

      OMIM: 123260

      KEGG: hsa:1401

      STRING: 9606.ENSP00000255030

      UniGene: Hs.709456



    1v1周青梅郑宣木天寥
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