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  • Your Good Partner in Biology Research

    Human angiotensin Ⅱ (ANG-Ⅱ) ELISA Kit

    • 中文名称:
      人血管紧张素Ⅱ(ANG-Ⅱ)ELISA Kit
    • 货号:
      CSB-E04493h
    • 规格:
      96T/48T
    • 价格:
      ¥3200/¥2500
    • 其他:

    产品详情

    • 产品描述:

      This Human ANG-Ⅱ ELISA Kit was designed for the quantitative measurement of Human ANG-Ⅱ protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

    • 别名:
      angiotensin Ⅱ, angiotensin 2, ANG2
    • 缩写:
      ANG-Ⅱ
    • 种属:
      Homo sapiens (Human)
    • 样本类型:
      serum, plasma, cell culture supernates, tissue homogenates
    • 检测范围:
      31.25 pg/mL-2000 pg/mL
    • 灵敏度:
      7.81 pg/mL
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Cardiovascular
    • 测定原理:
      quantitative
    • 测定方法:
      Sandwich
    • 说明书:
    • 本试剂盒所含材料:
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ANG-II antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled ANG-II antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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    • 本试剂盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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    • 数据处理:
    • 货期:
      3-5 working days

    产品评价



    1v1周青梅郑宣木天寥
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