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    Human Tissue Inhibitor Of Matrix Metalloprotease-1 (TIMP-1) ELISA KIT

    • 中文名称:
      人基质金属蛋白酶组织抑制因子1(TIMP-1)ELISA Kit
    • 货号:
      CSB-E08003h
    • 规格:
      96T/48T
    • 价格:
      ¥3600/¥2500
    • 其他:

    产品详情

    • 产品描述:

      The human tissue inhibitor of matrix metalloprotease-1 (TIMP1) ELISA kit is suitable for the quantitative determination of human TIMP1 in serum, plasma, or tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human TIMP1 levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample.

      TIMP1 is a glycoprotein produced by cardiac myocytes and fibroblasts in the normal heart. TIMP1 binds with active MMPs to inhibit MMP activity. In addition to suppressing MMP activity, TIMP1 also regulates multiple biological activities, including inhibiting apoptosis, stimulating cell proliferation and differentiation, as well as promoting migration and angiogenesis or the metastatic potential of tumor cells. Overexpression of TIMP1 has been found in several types of human cancers, including prostate cancer, lung cancer, melanoma, glioblastoma, and breast cancer. TIMP1 acting as a specific hallmark of human cancer is also associated with the tumor microenvironment (TME) and drug resistance.

    • 别名:
      Clgi ELISA Kit; Collagenase inhibitor ELISA Kit; Collagenase inhibitor; Human ELISA Kit; EPA ELISA Kit; EPO ELISA Kit; Erythroid Potentiating Activity ELISA Kit; Erythroid-potentiating activity ELISA Kit; Fibroblast collagenase inhibitor ELISA Kit; FLJ90373 ELISA Kit; HCI ELISA Kit; Human Collagenase Inhibitor ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Metalloproteinase inhibitor 1 precursor ELISA Kit; OTTHUMP00000023214 ELISA Kit; TIMP 1 ELISA Kit; TIMP ELISA Kit; TIMP metallopeptidase inhibitor 1 ELISA Kit; TIMP-1 ELISA Kit; Timp1 ELISA Kit; TIMP1 protein ELISA Kit; TIMP1_HUMAN ELISA Kit; Tissue Inhibitor of Metalloproteinase 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; Tissue inhibitor of metalloproteinases ELISA Kit; Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor ELISA Kit
    • 缩写:
      TIMP1
    • Uniprot No.:
    • 种属:
      Homo sapiens (Human)
    • 样本类型:
      serum, plasma, tissue homogenates
    • 检测范围:
      0.39 ng/mL-25 ng/mL
    • 灵敏度:
      0.434 ng/mL
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Cardiovascular
    • 测定原理:
      quantitative
    • 测定方法:
      Sandwich
    • 说明书:
    • 精密度:

       

      Intra-assay Precision (Precision within an assay): CV%<8%

      Three samples of known concentration were tested twenty times on one plate to assess.

      Inter-assay Precision (Precision between assays):CV%<10%

      Three samples of known concentration were tested in twenty assays to assess.

       

    • 线性度:

       

      To assess the linearity of the assay, samples were spiked with high concentrations of human Timp-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

       

       

    • 标准曲线:

       

      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

       

       

    • 数据处理:
    • 货期:
      3-5 working days

    产品评价

    靶点详情

    • 功能:
      Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
    • 基因功能参考文献:
      1. significantly increased in the serum of type 2 diabetes mellitus patients with diabetic retinopathy PMID: 29752166
      2. Study evaluated for the first time the plasma levels and the diagnostic usefulness of VEGF, MMP-9, and TIMP-1 in cervical cancer (CC), not only independently but especially in combination with established cervical tumor markers. All tested parameters showed statistical significance when compared their concentrations in patients with CC to healthy women. PMID: 30037277
      3. In monocytes, incubation with psoriasis' sera increased the production of MMP-9 and TIMP-1 in comparison with both baseline and monocytes incubated with non-psoriatic sera PMID: 29979898
      4. no difference in the expression of MMP-7 and TIMP-1 in the endometrium in relation to hormone levels and menstrual cycle phases were observed PMID: 30187906
      5. Increased matrix metalloproteinase-9/tissue inhibitor of metalloproteinase-1 ratio in smokers with airway hyperresponsiveness is associated with accelerated lung function decline. PMID: 29692608
      6. MMP-9 and TIMP-1 levels are correlated with age, pathological grade, tumor size and lymph node metastasis of breast cancer patients. PMID: 30010105
      7. IL-33 can regulate deposition of ECM and promote the process of pulmonary fibrosis by inducing the imbalance between MMP-9 and TIMP-1. PMID: 29417309
      8. Study found that TIMP-1 accelerates cell proliferation through YAP/TAZ activation in cancer. PMID: 28925394
      9. that TLR2 is involved in IL29-stimulated TIMP1 expression in Raw264.7 cells and primary macrophages. PMID: 29658577
      10. Results found that CK18, MMP-9, and TIMP1 averages of those with positive clinical lymph nodes and those in clinical stage 3 were higher than the averages of those with negative clinical lymph nodes and those in clinical stage 2. PMID: 29651326
      11. High serum tissue inhibitor of metalloproteinase-1 level is associated with gastric cancer. PMID: 30192205
      12. Gal-3 gene and protein expression levels were also significantly higher in lung tissue from an allergic asthma murine model using TIMP-1 knockout. PMID: 28992358
      13. In dilation cardiomyopathy, expression of MMP-2, MMP-9, and TIMP-1 and their ratios in autopsy material and in cultures was elevated by 1.5-9 times. PMID: 29504111
      14. High Co-expression of TIMP-1 and CD63 is associated with glioblastoma. PMID: 29523123
      15. plasma levels of TIMP-1 are associated with pancreatic lesion-induced cachexia in patients without jaundice. PMID: 29394913
      16. the expressions of TIMP1 can predict prognosis in astrocytic tumors PMID: 27258564
      17. It is well established by now that progression of melanoma depends on ECM remodeling, TIMPs family being one of the most important regulators of this process. PMID: 29250646
      18. Authors observed a significant increase in the immunoexpression of collagen I and III in patients with DCM and a significant reduction in the immunoexpression of MMP-1 compared with the control group. Also, the collagen I and TIMP-1 expression indicated a positive linear correlation and respectively a negative linear relationship with collagen III and MMP-1. PMID: 29250654
      19. Low TIMP1 expression is associated with cervical cancer. PMID: 30052166
      20. In III-rd trimester of pregnancy parameters of Timp-1 and Survivin - anti-apoptotic substances concentration were similar in maternal and cord blood in both artery and vein. We found no increased activity of selected antiapoptotic factors. PMID: 28509321
      21. Reduced expression of types I and III collagen and TIMP-1 as well as the increased expression of MMP-1 and MMP-8 in the anterior vaginal wall tissues play important roles in the onset of pelvic organ prolapse. PMID: 29263043
      22. no significant difference was detected in the expression level of individual MMP9 and TIMP1 genes in SCZ patients versus healthy controls either in total numbers of subject or in sex based subgroups PMID: 28578515
      23. Significant differences in gene expression profiles were observed between patients with post-kidney transplant bladder tumors and those with conventional bladder tumors. TIMP-l expression was significantly higher in first group than in the second. PMID: 29038419
      24. the key role of TIMP-1 for the anti-angiogenic properties of stimulated MSC secretome already observed in mouse is confirmed in human. PMID: 28739509
      25. corticosteroid-resistant patients have higher basal MMP-9 levels and produce more MMP-9 levels in response to IL-1beta. PMID: 28696822
      26. Increased expression of TIMP1 (for both mRNA and protein) associated with poor ulcer healing in Diabetic Foot Ulcers with infection. PMID: 28682675
      27. female patients exhibited lower methylation levels of MMP-9 but higher methylation levels of TIMP-1 compared with male patients, and the methylation levels of TIMP-1 gradually decreased with age. PMID: 29298087
      28. miR-516a-5p may regulate MTHFR, MMP-2, and TIMP-1 expressions in vascular smooth muscle cells, possibly promoting the disruption of homocysteine metabolism and proteolytic degradation of elastin for abdominal aortic aneurysm formation. PMID: 28288890
      29. C-type natriuretic peptide (CNP)administration significantly decreased the secretion and expression of MMP-2 and MMP-9 in the cultured mesangial cells ; (2) the secretion and expression of TIMP-1 progressively elevated after treatment with CNP for 24 and 48 h, whereas declined at later time point; (3) CNP expression was negatively correlated with MMP-2 and MMP-9 expression. PMID: 28554303
      30. TIMP-1 modulates leukemic blasts survival, migration and function via CD63/PI3K/Akt/p21 signaling. As a "bad actor" in a "bad soil", TIMP-1 is a potential novel therapeutic target in leukemic bone marrow microenvironment. PMID: 27903985
      31. CD82 is a component of the promiscuous TIMP-1 interacting protein complex on cell surface of human pancreatic adenocarcinoma cells. CD82 directly binds to TIMP-1 N-terminal region through its large extracellular loop and co-localizes with TIMP-1. PMID: 28030805
      32. these results show here that EGFR signaling induces TIMP-1 expression in colorectal cancer cells, and that TIMP-1 promotes a more aggressive behavior, specifically in KRAS mutated cells PMID: 27509063
      33. Our results provided evidence that polymorphisms in TIMP1, DLX1 and DLX2 genes may be associated with DF phenotypes. PMID: 28131910
      34. The plasma levels of interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha, tissue inhibitor of metalloproteinases (TIMP)-1 are increased in myelofibrosis (MF) patients. PMID: 27304059
      35. The present study suggests that scoring of TIMP-1 immunoreactivity is a better choice than measuring of TIMP-1 plasma levels. PMID: 27619981
      36. Results show that TIMP1 expression is significantly upregulated in human colon cancer. Its suppression decreases proliferation, and metastasis but increases apoptosis by inducing TIMP1 specific regulated FAK-PI3K/AKT and MAPK pathway. These results suggest that TIMP1 may play an important role in promoting tumorigenesis and metastasis of human colon cancer. PMID: 27644693
      37. Tissue inhibitor of matrix metalloproteinases 1 (TIMP1) inhibition resensitized tumors to gemcitabine and radiotherapy. PMID: 28765154
      38. Suggest that IL-6 could promote the invasiveness of breast cancer cells by inducing secretion of TIMP-1 and -2, causing a disturbance in TIMP/MMP balance. PMID: 28375112
      39. This study indicates that in our population, the COL4A3 rs55703767 polymorphism decreased the risk of KC. However, the TIMP-1 rs6609533 polymorphism was associated with an increased risk of KC. PMID: 28197741
      40. Low MMP-8/TIMP-1 reflects left ventricle impairment in takotsubo cardiomyopathy and high TIMP-1 may help to differentiate it from acute coronary syndrome PMID: 28278213
      41. Data show that the mean values for TIMP1, TIMP2 and MMP2 were lower in survivors, MMP9 was higher in survivors. PMID: 28192449
      42. We conclude that in the resected esophageal cancer an increased mRNA expression of MMP-7, MMP-10 and TIMP-1 correlated with clinicopathologic features. We suggest that these genes may play a role during progression of the diseaseMMP-10, MMP-7, TIMP-1, TIMP-2 were overexpressed in 73%, 85%, 55% and 42% of esophageal cancer samples, respectively. PMID: 28510611
      43. plasma concentrations of MMP-7, MMP-8, -9 and TIMP-1 within 96 h from the onset of acute pancreatitis symptoms are elevated in acute pancreatitis patients compared with healthy controls PMID: 27561093
      44. TIMMP1 expression is a major factor in myocardial fibrosis. PMID: 27113051
      45. High TIMP1 expression is associated with hepatic fibrosis. PMID: 28098912
      46. TIMP-1 was highly expressed in TNBC patients and was associated with a poor prognosis. PMID: 27130446
      47. Results suggest a crucial role of MMP9 at the early stage of carcinogenesis in the large intestine. The increase in MMP9 and TIMP1 mRNA concentration and the decrease in MMP28 in the large intestinal tissue may be a confirmation of cancer. PMID: 28293015
      48. Expression of TIMP1 is increased in chronic pancreatitis, pancreatic intra-epithelial neoplasia, and pancreatic ductal adenocarcinoma tissues from patients. TIMP1 signaling via CD63 leads to activation of hepatic stellate cells, which create an environment in the liver that increases its susceptibility to pancreatic tumor cells. PMID: 27506299
      49. Our study provides information on the dynamic changes of MMP-9-TIMP-1 system and S100B in the perioperative period. Preoperative reduction of TIMP-1 might be predictive for shunt requirement but future studies are required for verification. PMID: 27121520
      50. Serum TIMP1 did not differ significantly between normal weight, overweight, and obese participants. PMID: 27296149

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    • 亚细胞定位:
      Secreted.
    • 蛋白家族:
      Protease inhibitor I35 (TIMP) family
    • 组织特异性:
      Detected in rheumatoid synovial fluid (at protein level).
    • 数据库链接:

      HGNC: 11820

      OMIM: 305370

      KEGG: hsa:7076

      STRING: 9606.ENSP00000218388

      UniGene: Hs.522632



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