<menu id="ssssw"><table id="ssssw"></table></menu>
  • <rt id="ssssw"><samp id="ssssw"></samp></rt>
  • <bdo id="ssssw"></bdo>
  • Your Good Partner in Biology Research

    Human Tau proteins ELISA kit

    • 中文名称:
      人Tau蛋白ELISA kit
    • 货号:
      CSB-E12011h
    • 规格:
      96T/48T
    • 价格:
      ¥3600/¥2500
    • 其他:

    产品详情

    • 产品描述:

      The human Tau ELISA Kit is engineered for accurate measurement of human Tau levels from samples including serum, plasma, or cerebrospinal fluid (CSF). It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the Tau content in the sample. The color intensity is positively correlated with Tau content in the sample. The Tau concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

      Tau, also called MAPT, is the main component of neurofibrillary tangles (NFTs). As a stabilizing microtubule-associated protein (MAP), Tau primarily functions to protect microtubules (MTs) against depolymerization by decreasing the dissociation of tubulin at both MT ends, leading to an elevated growth rate and declined catastrophe frequency. In addition to being a stabilizer of microtubules, Tau is involved in multiple biological processes, including myelination, glucose metabolism, axonal transport, microtubule dynamics, iron homeostasis, neurogenesis, motor function, learning & memory, neuronal excitability, and DNA protection.

    • 别名:
      AI413597 ELISA Kit; AW045860 ELISA Kit; DDPAC ELISA Kit; FLJ31424 ELISA Kit; FTDP 17 ELISA Kit; G protein beta1/gamma2 subunit interacting factor 1 ELISA Kit; MAPT ELISA Kit; MAPTL ELISA Kit; MGC134287 ELISA Kit; MGC138549 ELISA Kit; MGC156663 ELISA Kit; Microtubule associated protein tau ELISA Kit; Microtubule associated protein tau isoform 4 ELISA Kit; Microtubule-associated protein tau ELISA Kit; MSTD ELISA Kit; Mtapt ELISA Kit; MTBT1 ELISA Kit; MTBT2 ELISA Kit; Neurofibrillary tangle protein ELISA Kit; Paired helical filament tau ELISA Kit; Paired helical filament-tau ELISA Kit; PHF tau ELISA Kit; PHF-tau ELISA Kit; PPND ELISA Kit; PPP1R103 ELISA Kit; Protein phosphatase 1, regulatory subunit 103 ELISA Kit; pTau ELISA Kit; RNPTAU ELISA Kit; TAU ELISA Kit; TAU_HUMAN ELISA Kit; Tauopathy and respiratory failure ELISA Kit; Tauopathy and respiratory failure, included ELISA Kit
    • 缩写:
      MAPT
    • Uniprot No.:
    • 种属:
      Homo sapiens (Human)
    • 样本类型:
      serum, plasma, cerebrospinal fluid (CSF)
    • 检测范围:
      15.6 pg/mL-1000 pg/mL
    • 灵敏度:
      3.9 pg/mL
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Signal Transduction
    • 测定原理:
      quantitative
    • 测定方法:
      Sandwich
    • 说明书:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%
      Three samples of known concentration were tested twenty times on one plate to assess.
      Inter-assay Precision (Precision between assays): CV%<10%
      Three samples of known concentration were tested in twenty assays to assess.
    • 线性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of human Tau in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
       SampleSerum(n=4)
      1:1Average %84
      Range %80-92
      1:2Average %97
      Range %92-106
      1:4Average %98
      Range %93-105
      1:8Average %93
      Range %86-98
    • 回收率:
      The recovery of human Tau spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample TypeAverage % RecoveryRange
      Serum (n=5) 10095-105
      EDTA plasma (n=4)9791-101
    • 标准曲线:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      pg/mlOD1OD2AverageCorrected
      10002.319 2.423 2.371 2.228
      5001.828 1.864 1.846 1.703
      2501.291 1.324 1.308 1.165
      1250.776 0.796 0.786 0.643
      62.50.464 0.498 0.481 0.338
      31.20.321 0.341 0.331 0.188
      15.60.215 0.227 0.221 0.078
      00.142 0.144 0.143  
    • 数据处理:
    • 货期:
      3-5 working days

    产品评价

    靶点详情

    • 功能:
      Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
    • 基因功能参考文献:
      1. genetic manipulation of Sirt3 revealed that amyloid-beta increased levels of total tau acetylated tau through its modulation of Sirt3. PMID: 29574628
      2. Data suggest that both the small heat shock protein HspB1/Hsp27 and the constitutive chaperone Hsc70/HspA8 interact with tau to prevent tau-fibril/amyloid formation. Chaperones from different families play distinct but complementary roles in prevention of tau-fibril/amyloid formation. (HspB1 = heat shock protein family B small member 1; Hsc70 = heat shock protein family A Hsp70) PMID: 29298892
      3. a 2.0-kDa peptide which biochemically and immunologically resembles the injected amino terminal tau 26-44 was endogenously detected in vivo, being present in hippocampal synaptosomal preparations from Alzheimer's disease subjects. PMID: 29508283
      4. Study reports the identification of new bona fide human brain circular RNAs produced from the MAPT locus. PMID: 29729314
      5. TAU attaches to brain lipid membranes where it self-assembles in a cation-dependent manner. PMID: 29644863
      6. Microtubule hyperacetylation enhances KL1-dependent micronucleation under a Tau deficiency in mammary epithelial cells. PMID: 30142893
      7. This article presents key studies of tau in oligodendrocytes and select important studies of tau in neurons. The extensive work on tau in neurons has considerably advanced the understanding of how tau promotes either health or disease. [review] PMID: 30111714
      8. Zn2 + enhances Tau aggregation-induced apoptosis and toxicity in neuronal cells. PMID: 27890528
      9. Tau binds to synaptic vesicles via its N-terminal domain and interferes with presynaptic functions. PMID: 28492240
      10. Study identifies a potential "two-hit" mechanism in which tau acetylation disengages tau from microtubules (MT) and also promotes tau aggregation. Thus, therapeutic approaches to limit tau K280/K281 acetylation could simultaneously restore MT stability and ameliorate tau pathology in Alzheimer's disease and related tauopathies. PMID: 28287136
      11. In vitro neuroprotective effects of naringenin nanoemulsion against beta-amyloid toxicity through the regulation of amyloidogenesis and tau phosphorylation. PMID: 30001606
      12. To confirm the neuroprotective role of 24-OH, in vivo experiments were run on mice that express human tau without spontaneously developing tau pathology (hTau mice), by means of the intracerebroventricular injection of 24-OH. PMID: 29883958
      13. These findings suggest a relative homogeneous clinicopathological phenotype in P301L MAPT mutation carriers in our series. This phenotype might help in the differential diagnosis from other tauopathies and be a morphological hint for genetic testing. The haplotype analysis results suggest a founder effect of the P301L mutation in this area. PMID: 28934750
      14. Report that the interaction of Tau with vesicles results in the formation of highly stable protein/phospholipid complexes. These complexes are toxic to primary hippocampal cultures and are detected by MC-1, an antibody recognizing pathological Tau conformations. The core of these complexes is comprised of the PHF6* and PHF6 hexapeptide motifs, the latter in a beta-strand conformation. PMID: 29162800
      15. more selective group of neurons appears to be affected in frontotemporal lobar degeneration (FTLD)-TDP and FTLD-FUS than in FTLD-tau PMID: 28984110
      16. Our data show that the hyperacetylation of Tau by p300 histone acetyltransferase (HAT) disfavors liquid-liquid phase separation , inhibits heparin-induced aggregation, and impedes access to LLPS-initiated microtubule assembly PMID: 29734651
      17. Because neurofibrillary tangles are aberrant intracellular inclusions formed in the AD patients by hyperphosphorylated tau, it was initially proposed that phosphorylated and/or aggregated intracellular tau protein was causative of neuronal death. However, recent studies suggest a toxic role for non-phosphorylated and non-aggregated tau when it is located in the brain extracellular space. [review] PMID: 29584657
      18. MAPT rs242557G/A genetic polymorphism is associated with susceptibility to sporadic AD, and individuals with a GG genotype of rs242557G/A might be at a lower risk. PMID: 29098924
      19. Study indicates that there are at least two common patterns of TDP-43 and tau protein misfolding in human brain aging. In patients lacking substantial Alzheimer's disease pathology, cerebral age-related TDP-43 with sclerosis (CARTS) cases tend to have tau neurofibrillary tangles in the hippocampal dentate granule neurons, providing a potential proxy indicator of CARTS. PMID: 28281308
      20. Patients with Kii amyotrophic lateral sclerosis and parkinsonism-dementia complex (Kii ALS/PDC) had dislocated, multinucleated Purkinje cells and various tau pathologies in the cerebellum. These cerebellar abnormalities may provide new insights into the pathomechanism of Kii ALS/PDC and may provide a neuropathological marker for the condition. PMID: 28236345
      21. The studies findings indicate that p.E372G is a pathogenic microtubule-associated protein tau mutation that causes microtubule-associated protein tau similar to p.G389R. PMID: 27529406
      22. Solven ionic strength, temperature and polarity altered tau conformation dynamics. PMID: 29630971
      23. MAPT alternative splicing is associated with Neurodegenerative Diseases. PMID: 29634760
      24. High tau expression is associated with blood vessel abnormalities and angiogenesis in Alzheimer's disease. PMID: 29358399
      25. We identified common splice factors hnRNP F and hnRNP Q regulating the haplotype-specific splicing of MAPT exon 3 through intronic variants rs1800547 and rs17651213 PMID: 29084565
      26. Cognitive impairment in progressive supranuclear palsy is associated with severity of progressive supranuclear palsy-related tau pathology. PMID: 29082658
      27. These observations indicate the ability of QUE to decrease tau protein hyperphosphorylation and thereby attenuate the associated neuropathology... these results support the potential of QUE as a therapeutic agent for AD and other neurodegenerative tauopathies. PMID: 29207020
      28. Increasing microtubule acetylation rescues human tau-induced microtubule defects and neuromuscular junction abnormalities in Drosophila. PMID: 28819043
      29. The findings reveal the ability of Bin1 to modify actin dynamics and provide a possible mechanistic connection between Bin1 and tau-induced pathobiological changes of the actin cytoskeleton. PMID: 28893863
      30. We find that both the generation of Abeta and the responsiveness of TAU to A-beta are affected by neuronal cell type, with rostral neurons being more sensitive than caudal neurons. PMID: 29153990
      31. The results of the current study indicate that variations in microtubule-associated protein tau influence cognition in progressive supranuclear palsy. PMID: 29076559
      32. The identification of mutations in MAPT, the gene that encodes tau, causing dementia and parkinsonism established the notion that tau aggregation is responsible for the development of disease. PMID: 28789904
      33. CSF tau proteins and their index differentiated between Alzheimer's disease or other dementia patients and cognitively normal subjects, while CSF levels of neurofilaments expressed as their index seem to contribute to the discrimination between patients with neuroinflammation and normal controls or AD patients PMID: 28947837
      34. Comparison of the distributions of tau pTyr18 and double-phosphorylated Syk in the transgenic mouse brain and human hippocampus showed that the phosphorylation of tyrosine 18 in tau already occurs at an early stage of tauopathy and increases with the progression of neurodegeneration. Syk appears unlikely to be a major kinase that phosphorylates tyrosine 18 of tau at the early stage of tauopathy. PMID: 28919467
      35. Study confirmed that Western diet did not exacerbate tau pathology in hTau mice, observed that voluntary treadmill exercise attenuates tau phosphorylation, and reported that caloric restriction seems to exacerbate tau aggregation compared to control and obese hTau mice. PMID: 28779908
      36. Study showed a gradual accumulation of nuclear tau in human cells during aging and its general co-localization with the DAPI-positive heterochromatin, which seems to be related to aging pathologies (neurodegenerative or cancerous diseases), where nuclear AT100 decreases drastically, a condition very evident in the more severe stages of the diseases. PMID: 28974363
      37. Methamphetamine can impair the endoplasmic reticulum-associated degradation pathway and induce neuronal apoptosis through endoplasmic reticulum stress, which is mainly mediated by abnormal CDK5-regulated Tau phosphorylation. PMID: 29705343
      38. Aha1 colocalized with tau pathology in brain tissue, and this association positively correlated with Alzheimer disease progression. PMID: 28827321
      39. Assessed the subcellular localization of tau45-230 fragment using tau45-230-GFP-transfected hippocampal neurons as well as neurons in which this fragment was endogenously generated under experimental conditions that induced neurodegeneration. Results suggested that tau45-230 could exert its toxic effects by partially blocking axonal transport along microtubules, contributing to the early pathology of Alzheimer's disease. PMID: 28844006
      40. frontotemporal dementia and parkinsonism linked to chromosome 17 tau with a mutation in the C-terminal region had different banding patterns, indicating a different phosphorylation pattern. PMID: 27641626
      41. Study demonstrated the presence of the smaller Tau isoform (352 amino acids), whose amount increases in differentiated SK-N-BE cells, with Tau-1/AT8 nuclear distribution related to the differentiation process. PMID: 29684490
      42. In primary-culture fetal astrocytes, streptozotocin increases phosphorylation of Tau at Ser396. alpha-boswellic acid reduced hyperphosphorylated tau (Ser404). Interruption in astroglial Reelin/Akt/Tau signaling pathways may have a role in Alzheimer disease. PMID: 27567921
      43. Screening of MAPT, GRN and CHCHD10 genes in Chinese patients with frontotemporal dementia (FTD) identified about 4.9% mutation carriers. Among the known FTD causative genes tested, MAPT and CHCHD10 play the most important roles in Chinese patients with sporadic FTD. PMID: 28462717
      44. Data show that aggregation of the Tau protein correlates with destabilization of the turn-like structure defined by phosphorylation of Ser202/Thr205. PMID: 28784767
      45. deletion or inhibition of the cytoplasmic shuttling factor HDAC6 suppressed neuritic tau bead formation in neurons. PMID: 28854366
      46. We propose that the H2 haplotype, which expresses reduced 4R tau compared with the H1 haplotype, may exert a protective effect as it allows for more fluid mitochondrial movement along axons with high energy requirements, such as the dopaminergic neurons that degenerate in PD. PMID: 28689993
      47. Results find that overexpression of hTau increases intracellular calcium, which in turn activates calpain-2 and induces degradation of alpha4 nAChR. PMID: 27277673
      48. when misfolded tau assemblies enter the cell, they can be detected and neutralized via a danger response mediated by tau-associated antibodies and the cytosolic Fc receptor tripartite motif protein 21 (TRIM21) PMID: 28049840
      49. stress granules and TIA-1 play a central role in the cell-to-cell transmission of Tau pathology. PMID: 27460788
      50. clinicopathologic study shows inter- and intra-familial clinicopathologic heterogeneity of FTDP-17 due to MAPT p.P301L mutation, including globular glial tauopathy in one patient. PMID: 27859539

      显示更多

      收起更多

    • 相关疾病:
      Frontotemporal dementia (FTD); Pick disease of the brain (PIDB); Progressive supranuclear palsy 1 (PSNP1); Parkinson-dementia syndrome (PARDE)
    • 亚细胞定位:
      Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Cell projection, dendrite. Secreted.
    • 组织特异性:
      Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
    • 数据库链接:

      HGNC: 6893

      OMIM: 157140

      KEGG: hsa:4137

      STRING: 9606.ENSP00000340820

      UniGene: Hs.101174



    1v1周青梅郑宣木天寥
    <menu id="ssssw"><table id="ssssw"></table></menu>
  • <rt id="ssssw"><samp id="ssssw"></samp></rt>
  • <bdo id="ssssw"></bdo>