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  • Your Good Partner in Biology Research

    Human Lipopolysaccharides,LPS ELISA Kit

    • 中文名稱:
      人脂多糖/內毒素(LPS)ELISA Kit
    • 貨號:
      CSB-E09945h
    • 規格:
      96T/48T
    • 價格:
      ¥3600/¥2500
    • 其他:

    產品詳情

    • 產品描述:

      This Human LPS ELISA Kit was designed for the quantitative measurement of Human LPS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .

    • 縮寫:
      LPS
    • 種屬:
      Homo sapiens (Human)
    • 樣本類型:
      serum, plasma, tissue homogenates
    • 檢測范圍:
      6.25 pg/mL-400 pg/mL
    • 靈敏度:
      1.56 pg/mL
    • 反應時間:
      1-5h
    • 樣本體積:
      50-100ul
    • 檢測波長:
      450 nm
    • 研究領域:
      Others
    • 測定原理:
      quantitative
    • 測定方法:
      Sandwich
    • 說明書:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%
      Three samples of known concentration were tested twenty times on one plate to assess.
      Inter-assay Precision (Precision between assays): CV%<10%
      Three samples of known concentration were tested in twenty assays to assess.
    • 線性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of human LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
       SampleSerum(n=4)
      1:1Average %88
      Range %82-94
      1:2Average %95
      Range %91-99
      1:4Average %92
      Range %87-96
      1:8Average %94
      Range %89-99
    • 回收率:
      The recovery of human LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample TypeAverage % RecoveryRange
      Serum (n=5) 9390-97
      EDTA plasma (n=4)8882-96
    • 標準曲線:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      pg/mlOD1OD2AverageCorrected
      4002.809 2.960 2.885 2.720
      2002.227 2.366 2.297 2.132
      1001.515 1.556 1.536 1.371
      500.789 0.755 0.772 0.607
      250.432 0.470 0.451 0.286
      12.50.303 0.325 0.314 0.149
      6.250.235 0.232 0.234 0.069
      00.164 0.165 0.165  
    • 本試劑盒所含材料:
      • An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
      • Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
      • Biotin-labeled LPS antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
      • Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
      • HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
      • Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away the unbound solution and non-specific substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. HRP catalyzes TMB, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips(For 96 wells) --- Seal the microplate when incubation.
      • An instruction manual

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    • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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    • 數據處理:
    • 貨期:
      3-5 working days

    引用文獻

    產品評價

    問答及客戶評論

     常見問題解答
    Q:

    I would like to know if this ELISA kit is SPECIFIC for LPS of Escherichia Coli
    I mean CSB-E09945h ELISA kit

    A:
    Thanks for your inquiry.
    CSB-E09945h detects the residual LPs in the human body due to infection.Pls let me know if you have any further questions. Thank you.
    Q:

    Could you share with me what the antibodies in this kit bind to CSB-E09945h?

    A:
    Thanks for your inquiry.
    CSB-E09945h
    Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.Pls let me know if you have any further questions. Thank you.
    Q:

    I have an interest in knowing product information "Human Lipopolysaccharides, LPS ELISA Kit" CSB-E09945h.
    It is used to measure circulating levels of Gut-Derived Serum Lipopolysaccharide?
    How many blood samples or individuals can be analyzed with the kit?

    A:
    Thanks for your inquiry.
    CSB-E09945h
    For a 96T kit, it can test 88 samples. If you run dupliacated wells, it can test 44 samples.
    Pls follow actual test result if you want to test Gut-Derived Serum Lipopolysaccharide.Pls let me know if you have any further questions. Thank you.


    1v1周青梅郑宣木天寥
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