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  • Your Good Partner in Biology Research

    Human Keratin, type I cytoskeletal 18 fragment (K18-Asp396)ELISA Kit

    • 中文名称:
      人细胞角蛋白18片段Asp396(K18-Asp396)ELISA Kit
    • 货号:
      CSB-E13696h
    • 规格:
      96T/48T
    • 价格:
      ¥3600/¥2500
    • 其他:

    产品详情

    • 产品描述:

      This Human CK 18M30 ELISA Kit was designed for the quantitative measurement of Human CK 18M30 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 mIU/mL-1000 mIU/mL and the sensitivity is 3.9 mIU/mL .

    • 别名:
      N/A
    • 缩写:
      CK 18M30
    • 种属:
      Homo sapiens (Human)
    • 样本类型:
      serum, plasma, tissue homogenates, cell lysates
    • 检测范围:
      15.6 mIU/mL-1000 mIU/mL
    • 灵敏度:
      3.9 mIU/mL
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Others
    • 测定原理:
      quantitative
    • 测定方法:
      Sandwich
    • 说明书:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<10%      
      Three samples of known concentration were tested in twenty assays to assess.    
                   
    • 线性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of human CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
        Sample Serum(n=4)  
      1:1 Average % 90  
      Range % 85-94  
      1:2 Average % 86  
      Range % 81-92  
      1:4 Average % 94  
      Range % 90-99  
      1:8 Average % 89  
      Range % 83-96  
    • 回收率:
      The recovery of human CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 95 90-100  
      EDTA plasma (n=4) 99 94-105  
                   
                   
    • 标准曲线:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      mIU/ml OD1 OD2 Average Corrected  
      1000 2.577 2.552 2.565 2.443  
      500 2.056 2.016 2.036 1.914  
      250 1.267 1.202 1.235 1.113  
      125 0.683 0.648 0.666 0.544  
      62.5 0.379 0.405 0.392 0.270  
      31.2 0.294 0.279 0.287 0.165  
      15.6 0.201 0.199 0.200 0.078  
      0 0.117 0.126 0.122    
    • 数据处理:
    • 货期:
      3-5 working days

    产品评价



    1v1周青梅郑宣木天寥
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