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  • Your Good Partner in Biology Research

    Human Interleukin 8,IL-8 ELISA KIT

    • 中文名称:
      人白介素8(IL-8/CXCL8)ELISA KIT
    • 货号:
      CSB-E04641h
    • 规格:
      96T/48T
    • 价格:
      ¥3600/¥2500
    • 其他:

    产品详情

    • 产品描述:

      This Human IL8 ELISA Kit was designed for the quantitative measurement of Human IL8 protein in serum, cell culture supernates, saliva, urine, cerebrospinal fluid (CSF), tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.110 pg/mL.

    • 别名:
      (Ala-IL-8)77 ELISA Kit; (Ser-IL-8)72 ELISA Kit; 9E3 ELISA Kit; Beta thromboglobulin like protein ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; CEF-4 ELISA Kit; chemokine; CXC motif; ligand 8 ELISA Kit; CXCL8 ELISA Kit; Emoctakin ELISA Kit; GCP-1 ELISA Kit; GCP/IL-8 protein I ELISA Kit; GCP/IL-8 protein II ELISA Kit; GCP/IL-8 protein III ELISA Kit; GCP/IL-8 protein IV ELISA Kit; GCP/IL-8 protein V ELISA Kit; GCP/IL-8 protein VI ELISA Kit; GCP1 ELISA Kit; Granulocyte chemotactic protein 1 ELISA Kit; IL-8 ELISA Kit; IL-8(1-77) ELISA Kit; IL-8(9-77) ELISA Kit; IL8 ELISA Kit; IL8/NAP1 form I ELISA Kit; IL8/NAP1 form II ELISA Kit; IL8/NAP1 form III ELISA Kit; IL8/NAP1 form IV ELISA Kit; IL8/NAP1 form V ELISA Kit; IL8/NAP1 form VI ELISA Kit; IL8_HUMAN ELISA Kit; Inteleukin 8 ELISA Kit; LECT ELISA Kit; LUCT ELISA Kit; Lymphocyte-derived neutrophil-activating factor ELISA Kit; LYNAP ELISA Kit; MDNCF ELISA Kit; MDNCF-b ELISA Kit; MDNCF-c ELISA Kit; MONAP ELISA Kit; Monocyte derived neutrophil activating peptide ELISA Kit; Monocyte derived neutrophil chemotactic factor ELISA Kit; Monocyte-derived neutrophil chemotactic factor ELISA Kit; Monocyte-derived neutrophil-activating peptide ELISA Kit; NAF ELISA Kit; NAP 1 ELISA Kit; NAP-1 ELISA Kit; NAP1 ELISA Kit; Neutrophil activating peptide 1 ELISA Kit; Neutrophil activating protein 1 ELISA Kit; Neutrophil-activating factor ELISA Kit; Neutrophil-activating protein 1 ELISA Kit; Protein 3 10C ELISA Kit; Protein 3-10C ELISA Kit; SCYB 8 ELISA Kit; SCYB8 ELISA Kit; Small inducible cytokine subfamily B member 8 ELISA Kit; T cell chemotactic factor ELISA Kit; T-cell chemotactic factor ELISA Kit; TSG 1 ELISA Kit; TSG1 ELISA Kit
    • 缩写:
      IL8
    • Uniprot No.:
    • 种属:
      Homo sapiens (Human)
    • 样本类型:
      serum, cell culture supernates, saliva, urine, cerebrospinal fluid (CSF), tissue homogenates, cell lysates
    • 检测范围:
      31.25 pg/mL-2000 pg/mL
    • 灵敏度:
      7.110 pg/mL
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Immunology
    • 测定原理:
      quantitative
    • 测定方法:
      Sandwich
    • 说明书:
    • 精密度:

       Intra-assay Precision (Precision within an assay): CV%<8%

      Three samples of known concentration were tested twenty times on one plate to assess.

      Inter-assay Precision (Precision between assays): CV%<10%

      Three samples of known concentration were tested in twenty assays to assess. 

    • 线性度:

       To assess the linearity of the assay, samples were spiked with high concentrations of human IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    • 标准曲线:

       These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    • 本试剂盒所含材料:
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-8 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labled IL-8 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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    • 本试剂盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Squirt bottle, manifold dispenser, or automated microplate washer.
      • Absorbent paper for blotting the microtiter plate.
      • 100ml and 500ml graduated cylinders.
      • Deionized or distilled water.
      • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL and pipette tips.
      • Timer
      • Test tubes for dilution.

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    • 数据处理:
    • 货期:
      3-5 working days

    引用文献

    产品评价

    问答及客户评论

     客户评论
    • Sample type: Cell culture supernatant
      Sample species: Human
      Review: The mRNA level expressions of pro-inflammatory cytokines, IL-1α, IL-6, IL-8 and TNF-α were all assessed by qRT-PCR. * P<0.05, ** P<0.01, ***P<0.001 compared with the indicated group.
      PMID: 30026098
    • Sample type:Plasma
      Sample species: Human
      Review: Interleukin. Data are presented as the mean ± SD or n (%). Analysis performed using t-test or Student’s t-test.
      PMID: 30008611
    • Sample type: Cell culture supernatant
      Sample species: Human
      Review: IL-8 in the conditioned medium has no significant differences among these groups.
      PMID: 29755530
    • Sample type: Cell culture supernatant
      Sample species: Human
      Review: Serum Effect of various molecular weights of HA (30, 300 and 1300 kDa) on P.gingivalis-stimulated cytokine expression in HGFs. IL-8 production were determined by ELISA. Each value represents the mean ± S.D. from three separate experiments.
      PMID: 30465936

    靶点详情

    • 功能:
      IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
    • 基因功能参考文献:
      1. VEGF and IL-8 play a prominent role in the pathogenesis of early forms of rosacea and the hemostasis system. PMID: 29578433
      2. High levels of LIFR in colorectal cancer (CRC) facilitated proliferation and migration of endothelial cells, resulting in an increase in angiogenic activity. Moreover, IL-8 was found to play a role in the LIFR induced angiogenesis. IL-8 levels were correlated with LIFR levels in CRC tissues, whereas depletion of IL-8 led to a reduced angiogenic activity of LIFR in CRC cells. PMID: 29751081
      3. PKC-delta isoform plays a crucial role in Tat-TLR4 signaling pathway to activate NF-kappaB and CXCL8 production. PMID: 28539656
      4. CXCL8 was a target of miR-204, and miR-204 suppression could not increase cell viability, migration, invasion, and EMT procedure when CXCL8 was silenced. PMID: 29402343
      5. Interleukin 8 - 845 T/C and + 781 C/T polymorphisms were analyzed. For + 781C/T locus, in the dominant genetic model, significant difference between TT vs. CC + CT genotypes that significantly had a protective role against periodontitis disease. Positive association between distribution of IL8 - 845 T/C alleles and risk of periodontitis disease. C allele of IL-8 - 845 increased the risk of periodontitis disease. PMID: 30078118
      6. These results suggest a direct involvement of IL-8-CXCR1/2 axes in GBM progression by promoting both cell proliferation and invasion and indirectly by promoting neovascularization in the form of vascular mimicry. PMID: 30086759
      7. These results show that AKIP1 is crucial in cervical cancer angiogenesis and growth by elevating the levels of the NF-kappaB-dependent chemokines CXCL1, CXCL2, and CXCL8. PMID: 29520695
      8. The extramembranous domain of HofQ (emHofQ) was shown to interact with various cytokines, of which IL-8 exhibited the strongest interaction. PMID: 30088437
      9. he protein expression levels of IL-8 were significantly decreased in SZ patients, but no significant difference in the mRNA levels of IL-8 was observed between SZ patients and NC subjects. PMID: 28476335
      10. immune system process is indispensable in the progression of disease in colon, and identifies that IL-8 and MMP-9 play potential critical roles for the progression. PMID: 30074183
      11. IL-8 production was significantly enhanced following treatment with both IL-17A and CSE, while treatment with either IL-17A or CSE alone caused only a slight increase in IL-8 production. PMID: 29463070
      12. Work identified IL-8 as a positive regulator of homotypic CIC formation via enhancing intercellular adhesion. PMID: 30021676
      13. V2O5 induction of CXCL8 and CXCL11 chemokines may lead to the appearance and perpetuation of an inflammatory reaction into the dermal tissue. Further studies are required to evaluate dermal integrity and manifestations in subjects occupationally exposed, or living in polluted areas. PMID: 29901202
      14. Study findings point out that PAR2 could play an essential role in gastroesophageal reflux disease (GERD) pathogenesis - even repeated short-term exposure to weakly acidic conditions lead to the upregulation of PAR2 and subsequent activation of the intense IL-8 release in the esophageal mucosa and initiation of mucosal immune response in GERD. PMID: 29672302
      15. Given that IL-8, MIP-1beta, and MCP-1 are chemokines that play important roles in recruitment of immunocompetent cells for immune defense and tumor cell clearance, the observed lower levels of these markers with increasing PM2.5 exposure may provide insight into the mechanism by which DEE promotes lung cancer. PMID: 29023999
      16. These results suggested that stemness induction in SKOV3 cells by macrophages co-cultured with SKOV3-derived OCSLCs involved IL-8/STAT3 signaling. PMID: 29656182
      17. IL-8-251T>A (rs4073) Polymorphism is associated with gastric Cancer. PMID: 30275190
      18. expression level of CXCL8 had a positive relationship with recurrence probability in Acute myeloid leukemia. PMID: 29596823
      19. These data were in close agreement with the reduced cell migration and colony formation. Results from the present study suggested that reparixin and SCH527123 may be promising therapeutic agents for the treatment of pancreatic cancer by inhibiting the IL8/CXCR1/2 signaling cascade. PMID: 29749433
      20. A urinary IL-8 level of less than 61.25 pg/ml is more sensitive for prediction of complete remission in idiopathic membranous nephropathy patients. PMID: 29415357
      21. berberine inhibited the expression of MCP-1 and IL-8 induced by LPS. PMID: 28852897
      22. Regarding the IL-8 promoter T - 251A, the TA and AA genotypes were associated with significantly decreased risks of nasopharyngeal carcinoma (NPC) in a Taiwanese population compared with the wild-type TT genotype. The mRNA and protein expression levels for NPC tissues revealed no significant associations among the 20 NPC samples with different genotypes. PMID: 30200105
      23. IL-8 +781 T/C polymorphism is associated with the severe Clostridium difficile infection PMID: 29203364
      24. ShRNA mediated down-regulation of CXCL8 resulted in inhibition of cell proliferation, viability and invasion in vitro and a near complete growth reduction of tumor in vivo. PMID: 29679563
      25. CSF IL-8 concentrations were significantly elevated in CNS tumor patients as compared to non-tumoral individuals. AUC for CSF IL-8 was higher than for its index (CSF IL-8/serum IL-8). PMID: 29086194
      26. High IL8 expression is associated with melanoma. PMID: 29286146
      27. Lipo-CPFX, but not CPFX, retained the anti-IL-8 releasing activity. PMID: 29337216
      28. The results indicate significant contribution of IL8 on survival of hormonal dependent early-stage breast cancer patients and association with established parameters such as estrogen receptors/progesterone receptor and HER2. PMID: 28569250
      29. the frequency of non-classical monocytes expressing CXCL8 was increased in systemic sclerosis patient and monocytes expressing CXCL8 PMID: 29127442
      30. ntermediate Molecular Mass Hyaluronan and CD44 interactions enhanced normal PMN phagocytosis and IL-8 production PMID: 28730511
      31. serum levels in active vitiligo significantly elevated compared to those in stable vitiligo patients PMID: 29115683
      32. High IL8 expression is associated with pancreatic adenocarcinoma. PMID: 29205349
      33. Compared with controls, the interleukin (IL)-8 A/A genotype was more common in acute pancreatitis (AP). PMID: 29215544
      34. The presence of neither the first transmembrane helix of the receptor nor the lipid bilayer significantly affected the interactions of IL-8 with Binding Site-I of CXCR1. PMID: 29143165
      35. CXCL8 is highly expressed in cervical cancer tissues and cell lines, and correlated with malignant status and prognosis in cervical cancer patients. PMID: 28883082
      36. In conclusion, to our knowledge, this is the first study in the association of rs4073 and rs2227306 polymorphisms with childhood asthma risk in the Tunisian population. PMID: 28993876
      37. Results show that IL8 expression level is regulated by APE1 which activates NF-KB. PMID: 27388124
      38. aberrant miR-520c-3p expression may lead to reduced IL-8 expression and promote the mesenchymal phenotype in breast cancer cells, thereby increasing invasive growth. PMID: 29048659
      39. increased levels of IL-8 are associated with factors of worse prognosis in ovarian cancer PMID: 28872976
      40. Significantly elevated blood levels of IL-8 in myelodysplastic syndrome patients. PMID: 28856536
      41. the elevated concentrations of CXCL13, CXCL8, and CXCL10 or their increasing CSF/serum ratios may be potential biomarkers of neurosyphilis PMID: 27650493
      42. Results implicated the important role of PRL-3 in glycolysis metabolism through improving IL-8 secretion in colorectal cancer cells, and PRL-3 mediated glycolysis contributed to the promotion of cancer metastasis. PMID: 28791350
      43. Changes in serum IL-8 levels could be used to monitor and predict clinical benefit from immune checkpoint blockade in melanoma and NSCLC patients PMID: 28595336
      44. Lung cancer patients showed significantly lower levels of serum VEGF (1.9 fold) and IL-8 (~9 fold) than COPD patients. VEGF level was significantly higher (2.6 fold) in metastatic than non-metastatic cancer patients. An increase in MMP-9 (~1.6 fold) levels was observed in lung cancer patients. PMID: 27811960
      45. CXCL1/8 secreted by adipose-derived mesenchymal stem cells could promote breast cancer angiogenesis. PMID: 28514506
      46. our meta-analysis suggests that the IL-8 rs4073, A2767T, T11722T2, rs2234671, rs2230054, rs1126579, rs2227306, rs2227307, rs2227532, and T-738A polymorphisms are not associated with periodontitis while the IL-8 C1633T and rs1126580 polymorphisms may elevate the susceptibility of periodontitis based on the currently available evidences. PMID: 28446725
      47. An analogue of human CXCL8, CXCL8(3-72)K11R/G31P (hG31P) has been developed. PMID: 28754019
      48. inflammation triggered property of Microcystin-LR via IL-8/CXCR2 signaling PMID: 29197248
      49. A high IL-8 content in urine sampled on day 1 after renal transplantation was positively correlated with the activity of metalloproteinase-9 in urine. This proves that both of these chemokines cooperate in ischaemia-reperfusion injuries in transplanted kidneys. PMID: 28494217
      50. We discovered that IL-8 secreted from decidual stromal cells is a key cytokine enhancing the invasiveness of trophoblasts PMID: 28328096

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    • 亚细胞定位:
      Secreted.
    • 蛋白家族:
      Intercrine alpha (chemokine CxC) family
    • 数据库链接:

      HGNC: 6025

      OMIM: 146930

      KEGG: hsa:3576

      STRING: 9606.ENSP00000306512

      UniGene: Hs.624



    1v1周青梅郑宣木天寥
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