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  • Your Good Partner in Biology Research

    Human Hyaluronic acid,HA ELISA Kit

    • 中文名称:
      人透明质酸(HA)ELISA Kit
    • 货号:
      CSB-E04805h
    • 规格:
      96T/48T
    • 价格:
      ¥3600/¥2500
    • 其他:

    产品详情

    • 产品描述:

      This Human Hyaluronic acid (HA) ELISA Kit was designed for the quantitative measurement of Human Hyaluronic acid (HA) protein in serum, plasma, cerebrospinal fluid (CSF), tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

    • 别名:
      N/A
    • 缩写:
      HA
    • 种属:
      Homo sapiens (Human)
    • 样本类型:
      serum, plasma, cerebrospinal fluid (CSF), tissue homogenates
    • 检测范围:
      0.156 ng/mL-10 ng/mL
    • 灵敏度:
      0.039 ng/mL
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Cell Biology
    • 测定原理:
      quantitative
    • 测定方法:
      Sandwich
    • 说明书:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%
      Three samples of known concentration were tested twenty times on one plate to assess.
      Inter-assay Precision (Precision between assays): CV%<10%
      Three samples of known concentration were tested in twenty assays to assess.
    • 线性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of human HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
       SampleSerum(n=4)
      1:20Average %87
      Range %80-94
      1:40Average %92
      Range %88-99
      1:80Average %97
      Range %91-100
      1:160Average %101
      Range %97-105
    • 回收率:
      The recovery of human HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample TypeAverage % RecoveryRange
      Serum (n=5) 10095-105
      EDTA plasma (n=4)9388-98
    • 标准曲线:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      ng/mlOD1OD2AverageCorrected
      102.727 2.700 2.714 2.626
      52.231 2.345 2.288 2.200
      2.51.558 1.655 1.607 1.519
      1.250.831 0.901 0.866 0.778
      0.6250.477 0.451 0.464 0.376
      0.3120.301 0.322 0.312 0.224
      0.1560.189 0.174 0.182 0.094
      00.087 0.089 0.088  
    • 本试剂盒所含材料:
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human HA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-labeled HA antibody(100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
      • HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
      • Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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    • 本试剂盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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    • 数据处理:
    • 货期:
      3-5 working days

    引用文献

    产品评价

    问答及客户评论

     常见问题解答
    Q:

    We are looking into the best method to distinguish HA manufactured by fermentation and nature human HA.

    A:
    Thanks for your inquiry.
    CSB-E04805h Human Hyaluronic acid, HA ELISA Kit
    This kit can detect nature HA in polymerized form. As for HA manufactured by fermentation, it usually exists in free form.
    We think it can not be detected out by the kit.


    1v1周青梅郑宣木天寥
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