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    Human Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I ELISA Kit

    • 中文名称:
      人Ⅰ型胶原交联羧基末端肽(CTX-I)ELISA Kit
    • 货号:
      CSB-E11224h
    • 规格:
      96T/48T
    • 价格:
      ¥3200/¥2500
    • 其他:

    产品详情

    • 产品描述:

      This Human CTX-I ELISA Kit was designed for the quantitative measurement of Human CTX-I protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

    • 别名:
      Cross-linked Carboxy-terminal telopeptide of type 1 collagen, CTX-1, Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I
    • 缩写:
      CTX-I
    • 种属:
      Homo sapiens (Human)
    • 样本类型:
      serum, plasma, tissue homogenates
    • 检测范围:
      0.625 ng/mL-40 ng/mL
    • 灵敏度:
      0.156 ng/mL
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Others
    • 测定原理:
      quantitative
    • 测定方法:
      Sandwich
    • 说明书:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<10%      
      Three samples of known concentration were tested in twenty assays to assess.    
                   
    • 线性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of human CTX-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
        Sample Serum(n=4)  
      1:1 Average % 88  
      Range % 83-95  
      1:2 Average % 95  
      Range % 91-98  
      1:4 Average % 97  
      Range % 90-103  
      1:8 Average % 96  
      Range % 91-101  
    • 回收率:
      The recovery of human CTX-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 85 81-92  
      EDTA plasma (n=4) 96 93-106  
                   
                   
    • 标准曲线:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      ng/ml OD1 OD2 Average Corrected  
      40 2.995 2.960 2.978 2.838  
      20 2.557 2.570 2.564 2.424  
      10 1.666 1.765 1.716 1.576  
      5 0.839 0.886 0.863 0.723  
      2.5 0.491 0.524 0.508 0.368  
      1.25 0.263 0.251 0.257 0.117  
      0.625 0.201 0.212 0.207 0.067  
      0 0.138 0.142 0.140    
    • 数据处理:
    • 货期:
      3-5 working days

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    问答及客户评论

     常见问题解答
    Q:

    Please provide the following information regarding Human Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I ELISA Kit CSB-E11224h
    a. Is the capture antibody a monoclonal or polyclonal?
    b. What is the host of the capture antibody?
    c. Is the detection antibody a monoclonal or polyclonal?
    d. What is the host of the detection antibody?
    e. B208. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
    f. If recombinant protein, is it full length or partial?
    g. What is the species of the standard– human or something else?

    A:
    Thanks for your inquiry.
    CSB-E11224h
    Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
    Standard is synthetic peptide.
    Pls let me know if you have any further questions. Thank you.


    1v1周青梅郑宣木天寥
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