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  • Your Good Partner in Biology Research

    Human Cortisol ELISA Kit

    • 中文名称:
      人皮质醇(Cortisol)ELISA Kit
    • 货号:
      CSB-E05111h
    • 规格:
      96T/48T
    • 价格:
      ¥3600/¥2500
    • 其他:

    产品详情

    • 产品描述:

      This Human COR ELISA Kit was designed for the quantitative measurement of Human COR protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.049 ng/mL-200 ng/mL and the sensitivity is 0.049 ng/mL.

    • 缩写:
      COR
    • 种属:
      Homo sapiens (Human)
    • 样本类型:
      serum, plasma, cell culture supernates, tissue homogenates
    • 检测范围:
      0.049 ng/mL-200 ng/mL
    • 灵敏度:
      0.049 ng/mL
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Metabolism
    • 测定原理:
      quantitative
    • 测定方法:
      Competitive
    • 说明书:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<10%      
      Three samples of known concentration were tested in twenty assays to assess.    
                   
    • 线性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of human cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
        Sample Serum(n=4)  
      1:1 Average % 92  
      Range % 86-99  
      1:2 Average % 97  
      Range % 92-103  
      1:4 Average % 85  
      Range % 81-89  
      1:8 Average % 90  
      Range % 83-96  
    • 回收率:
      The recovery of human cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 90 87-92  
      EDTA plasma (n=4) 96 92-101  
                   
                   
    • 标准曲线:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      ng/ml OD1 OD2 Average    
      200 0.175 0.180 0.178    
      50 0.246 0.231 0.239    
      12.5 0.401 0.423 0.412    
      3.12 0.661 0.682 0.672    
      0.78 1.010 1.050 1.030    
      0.195 1.316 1.306 1.311    
      0.049 1.595 1.614 1.605    
      0 1.862 1.922 1.892    
    • 本试剂盒所含材料:
        • A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-human COR antibody.
        • Two vails Lyophilized Standard -- Dilute one of the standards at dilution series, read the OD values, and then draw a standard curve.
        • Anti-human COR Antibody (100 x concentrate) 1 x 60 μl-- Act as the detection antibody.
        • Antibody Diluent 1 x 10 ml -- Dilute the COR antibody solution.
        • HRP-conjugate (100 x concentrate) 1 x 120 μl-- Bind to the COR antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
        • HRP-conjugate Diluent 1 x 20 ml -- Dilute the HRP-conjugate solution.
        • Sample Diluent 2 x 20 ml -- Dilute the standard and sample solutions. Wash Buffer (25 x concentrate) 1 x 20 ml -- Wash away unbound or free substances.
        • TMB Substrate 1 x 10 ml -- HRP catalyzes the TMB, eliciting a chromogenic reaction.
        • Stop Solution 1 x 10 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
        • Four Adhesive Strips (For 96 wells)
        • An Instruction manual

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    • 本试剂盒不含材料:
        • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
        • An incubator that can provide stable incubation conditions up to 37°C±5°C.
        • Centrifuge
        • Vortex
        • Squirt bottle, manifold dispenser, or automated microplate washer
        • Absorbent paper for blotting the microtiter plate
        • 50-300ul multi-channel micropipette
        • Pipette tips
        • Single-channel micropipette with different ranges
        • 100ml and 500ml graduated cylinders
        • Deionized or distilled water
        • Timer
        • Test tubes for dilution

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    • 数据处理:
    • 货期:
      3-5 working days

    引用文献

    产品评价

    问答及客户评论

     常见问题解答
    Q:

    I would like to know if corticosterone also affects to OD value.

    A:
    Thanks for your inquiry.
    This kit is specific to Cortisol.It has no correlation with corticosterone and the corticosterone does not affect OD value.
    Q:

    I hope you would be able to advice me which kit would
    be suitable for me to purchase in order to conduct human salivary
    ELISA assay on cortisol. Sample size is 60 and running in
    duplicates.

    A:
    Thanks for your inquiry.
    We have CSB-E05111h available. It is based on competitive method. It is suitable to test human salivary. Pls folow actual test result.
    One 96T kit can test 43 samples if you run in duplicates.
     客户评论
    • Sample type: Plasma
      Sample species: Human
      Review: Cortisol levels in Experiment 2. Food, n = 21; Fasting, n = 24. b Cortisol levels in Experiment 4. Food, n = 22; Fasting, n = 23. Blue dots and red dots represent individuals in the food group and fasting group, respectively.
      PMID: 30301955
    • Sample type: Plasma
      Sample species: Human
      Review: Data are expressed as means SEM, except plasma cortisol and epinephrine concentration, which are descripted as median (inter-quartile range, IQR). FEV1, forced expiratory volume in one second; FVC, forced vital capacity.
      PMID: 30937140
    • Sample type: Saliva
      Sample species: Human
      Review: Cortisol responses to the Trier Social Stress Test by bullying group. Cortisol was measured in nanograms per milliliter (ng/ml), and was log-transformed in this figure. Figure shows means for cortisol levels, for physical bullied, verbal bullied, relational bullied, the total bullied and nonbullied children.
      PMID: 29196916
    • Sample type: Serum
      Sample species: Human
      Review: FPG, fasting plasma glucose; PPG, postprandial plasma glucose; BMI, body mass index; p < 0.05 denoted as a statistically significant (in bold). *Student t test, *Type 1 diabetes vs controls p < 0.05, #Type 1 vs Type 2 diabetes, p < 0.05, **Type 2 diabetes vs controls p < 0.05.
      PMID: 30184546


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