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  • Your Good Partner in Biology Research

    Human Alpha-fetoprotein,AFP ELISA Kit

    • 中文名称:
      人甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit
    • 货号:
    • 规格:
    • 价格:
    • 其他:


    • 产品描述:

      This Human AFP ELISA Kit was designed for the quantitative measurement of Human AFP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.563 ng/mL-100 ng/mL and the sensitivity is 1.332 ng/mL.

    • 别名:
      Afp ELISA Kit; AFPD ELISA Kit; Alpha fetoglobulin ELISA Kit; Alpha fetoprotein ELISA Kit; Alpha fetoprotein precursor ELISA Kit; Alpha-1-fetoprotein ELISA Kit; Alpha-fetoglobulin ELISA Kit; Alpha-fetoprotein ELISA Kit; alpha-fetoprotein; Hereditary persistence of; included ELISA Kit; FETA ELISA Kit; FETA_HUMAN ELISA Kit; Hereditary persistence of alpha fetoprotein ELISA Kit; HPAFP ELISA Kit
    • 缩写:
    • Uniprot No.:
    • 种属:
      Homo sapiens (Human)
    • 样本类型:
      serum, plasma, cell culture supernates, tissue homogenates
    • 检测范围:
      1.563 ng/mL-100 ng/mL
    • 灵敏度:
      1.332 ng/mL
    • 反应时间:
    • 样本体积:
    • 检测波长:
      450 nm
    • 研究领域:
    • 测定原理:
    • 测定方法:
    • 说明书:
    • 精密度:


      Intra-assay Precision (Precision within an assay): CV%<8%

      Three samples of known concentration were tested twenty times on one plate to assess.

      Inter-assay Precision (Precision between assays):CV%<10%

      Three samples of known concentration were tested in twenty assays to assess.


    • 线性度:


      To assess the linearity of the assay, samples were spiked with high concentrations of human AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    • 标准曲线:

      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    • 数据处理:
    • 货期:
      3-5 working days



    • 功能:
      Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
    • 基因功能参考文献:
      1. Peak preoperative serum alpha-fetoprotein to total tumor volume predicts recurrence of hepatocellular carcinoma. PMID: 29284589
      2. the results suggest that AFP may positively regulate cell proliferation by enhancing the apoptosis resistance via effect on TGF-beta and p53/Bax/caspase-3 signaling pathway in HepG2 cells. As such, the knockdown of AFP gene should be further investigated in vivo as a novel approach to hepatocellular carcinoma treatment. PMID: 30249880
      3. Aberrant CTNNB1 expression was seen in a substantial proportion of our hepatocellular carcinoma (HCC) cases. CTNNB1-positive HCC was associated with normal AFP levels, unicentric tumors, well-differentiated histology, and an unfavorable outcome. PMID: 30082549
      4. Elevated preoperative serum alpha-fetoprotein levels have predictive value for hepatocellular carcinoma survival. PMID: 28893428
      5. Cases of ovarian ovarian Sertoli-Leydig cell tumors (SLCTs) with elevated serum AFP should be sampled extensively to look for foci of intestinal-type glands, the likely source of the alpha fetoprotein (AFP) elevation in some of these neoplasms. PMID: 28582340
      6. In HBV-related cirrhosis, the combination of miR-122, AFP and PIVKA-II enables the identification of patients at higher risk of hepatocellular carcinoma development. PMID: 28650134
      7. Examination of AFP over time has great predictive and prognostic value for managing advanced gastric cancer. For those with markedly elevated AFP, triplet regimens may be a better choice. PMID: 29375212
      8. Low expression of AFP is associated with recurrence of hepatocellular carcinoma. PMID: 29572833
      9. High-levels of PIVKA-II in at-risk patients is an indicator of hepatocellular carcinoma (HCC)development in two-year time. CONCLUSIONS: Our data showed that PIVKA-II effectively increases the detection rate of HCC was a valid complement to AFP and image examination in HCC surveillance PMID: 28863782
      10. Unspecific AFP elevations occur in about 2% of pure testicular seminoma patients. PMID: 28668957
      11. serum level in the second trimester an important indicator of fetal surface malformations PMID: 27771626
      12. HCC [hepatocellular carcinoma] patients who are sero-positive for DCP [Des-gamma-carboxyprothrombin] and sero-negative for AFP[Alpha-fetoprotein] have significantly higher levels of serum ALT[alanine aminotransferase]; serum ALT levels may be of diagnostic importance in AFP-negative, HBV-related HCC [hepatitis B virus-related hepatocellular carcinoma] patients. PMID: 29192630
      13. Higher baseline serum AFP levels independently predicted a lower sustained viral response to antivirals among patients with chronic hepatitis C. PMID: 17713163
      14. Computer modeling shows the identification of potential binding sites of lysophospholipids (LP) receptors on the AFP third domain receptor binding fragment. AFP might bind not only to the LPL receptors, but also to LPLs themselves since AFP binds medium and long chain fatty acids. It is proposed that some of the activities ascribed to AFP might be due in part to the presence of bound LPLs and/or their receptors. [review] PMID: 26844573
      15. AFP may be an important molecule acting against paclitaxel-inhibited proliferation and induced apoptosis in hepatocellular carcinoma cells via repressing the activity of caspase-3 and stimulating the expression of Ras and Survivin. PMID: 27255186
      16. PIVKA II, when combined with AFP, may be considered as a screening test for hepatocellular carcinoma due to its high negative predictive value. PMID: 28652441
      17. Identification of the distinct impact of circulating tumor derived AFP on NK-cell function and viability may be crucial to developing a strategy to ameliorate hepatocellular carcinoma patient NK-cell functional deficits. PMID: 28468916
      18. The detection of ALT, AFP, AFP-L3, and GP73 has a certain guiding significance to predict the risk of hepatocellular carcinoma in hepatic cirrhosis patients PMID: 28540298
      19. Antibody therapy targeted at AFP-producing tumor cells showed an inhibitory effect on the PI3K/AKT pathway via the liberation, restoration and functional stabilization of PTEN. PTEN simultaneously induced both P53 activation and intracellular translocation of GLUT1, since these are closely associated with PTEN. PMID: 28498467
      20. Incorporation AFP status into the BCLC staging system to modify the recommended treatment of patients with stage C hepatocellular carcinoma. PMID: 27835609
      21. we used alpha-fetoprotein (AFP), a specific liver cancer marker, as an example to determine whether peptide-MHC complexes can be targets for CAR T-cell therapy against solid tumors.in an established intraperitoneal liver cancer xenograft model, AFP-CAR T cells showed robust antitumor activity PMID: 27535982
      22. miR-620, miR-1236 and miR-1270 are critical factors in the post-transcriptional regulation of AFP in hepatitis B infected hepatoma cells. Icaritin can counteract the effect of HBV. PMID: 27835879
      23. BWS registries and prospective clinical studies will assess the most appropriate means to screen for hepatoblastoma, whether aFP-screened patients have additional survival benefit over patients screened by US only, and whether the outcome is improved by earlier detection PMID: 28211991
      24. p55PIK significantly stimulated the expression of AFP by activating NF-kappaB signaling pathway in hepatocellular carcinoma cells. PMID: 28970114
      25. Irregular MIP pattern by CEUS and high level of serum AFP-L3 were independent risk factors for poor outcome after successful RFA. The Patients with these findings should be considered as special high-risk group in early-stage HCC. PMID: 27748052
      26. our data suggest that HBeAg seroconversion and liver cirrhosis are associated with the occurrence of HCC and that a surveillance system with regular measurement of AFP to watch for the occurrence of HCC may be mandatory for children and young patients with chronic HBV infection. PMID: 27748053
      27. We could demonstrate in our cohort the superior detection of early stage HCC with the combined use of the respective biomarkers and in particular GALAD even in AFP-negative tumors. PMID: 27936479
      28. A correlation was found between serum AFP concentration and age in extremely low-birthweight infants (ELBWI), and the 95%CI of serum AFP concentration was determined for ELBWI up to 2 years after birth. PMID: 27400853
      29. rAFP promoted growth of the human HCC cells, antagonize paclitaxel inhibition of HCC cell proliferation, suppress expression of active caspase-3, and promote expression of Ras and survivin. PMID: 28554567
      30. Measurements of serum alpha-fetoprotein (AFP) levels could be helpful in detecting progression or recurrence in treated patients with hepatitis B virus (HBV) - hepatocellular carcinoma (HCC) and normal alanine aminotransferase (ALT). PMID: 27304617
      31. AFP mRNA level is in enriched circulating tumor cells from patients with metastatic hepatocellular carcinoma. PMID: 27160647
      32. High serum AFP levels were significantly associated with the prevalence of metabolic syndrome in a Chinese asymptomatic population. PMID: 27121855
      33. Contrary to expectation, Chronic Hepatitis B patients with current or recent exposure to NA therapy showed poorer performance of AFP during hepatocellular carcinoma surveillance PMID: 27997559
      34. Protein induced by vitamin K absence or antagonist-II showed higher area under the curve than alpha-fetoprotein for diagnosis of hepatitis B virus-related hepatocellular carcinoma patients (0.901 vs 0.788).We conclude that Combining alpha-fetoprotein and protein induced by vitamin K absence or antagonist-II may improve the diagnostic value for early detection of hepatocellular carcinoma. PMID: 28621228
      35. AFP expression turned on prior to expression of Oct4, Klf4, Sox2 and c-myc, and the stemness markers CD44, CD133 and EpCAM in the normal human liver L-02 cell line or CHL cell lines upon transfection with MCV-HBx vectors. HBx stimulates AFP expression to induce natural reprogramming of liver cells, and AFP plays a critical role in promoting the initiation of HCC progenitor/stem cells. PMID: 27925189
      36. analysis of alpha-fetoprotein producing early gastric cancer with enteroblastic differentiation PMID: 27688662
      37. Lower post-treatment AFP levels and HCV genotype 2 significantly correlated with liver fibrosis regression after sustained virological response. PMID: 27123974
      38. Increased expression of AFP resulted in the upregulation of beta-catenin mRNA and protein levels, while knockdown of AFP with AFP shRNA led to significantly decreased beta-catenin mRNA and protein levels in hepatocellular carcinoma cells. PMID: 27924522
      39. serum level differentiates hepatocellular carcinoma from liver cirrhosis, especially when combined with acarboxyprothrombin PMID: 28296720
      40. soluble alpha-fetoprotein is a marker for Fanconi anemia; FANC proteins form multi-protein complexes with BRCA proteins and are involved in homologous DNA repair [review] PMID: 27690720
      41. Studies indicate that the experimental data on the carboxy-terminal third domain of alpha-fetoprotein (AFP-3D) interaction with cell cycle proteins in cultured MCF-7 breast cancer cells were consistent with the "in silico" findings. PMID: 27448301
      42. we evaluated the clinical performance of LOCItrade mark-based tumor marker assays CEA, CA19-9, CA15-3, CA125 and AFP in patients with gastrointestinal cancer and demonstrated their high diagnostic power PMID: 28011514
      43. The definitive endoderm and foregut endoderm differentiation capabilities of Wnt pathway-modulated cells were determined based on the expression levels of the endodermal transcription factors SOX17 and FOXA2 and those of the transcription activator GATA4 and the alpha-fetoprotein (AFP) gene, respectively. PMID: 26861571
      44. Suggest elevated serum AFP levels may have diagnostic value for early stage hepatocellular carcinoma in Korean patients. PMID: 26767857
      45. AFP promoted hepatocellular carcinoma cell invasion and metastasis via up-regulating expression of metastasis-related proteins. PMID: 26756858
      46. Interference of AFP reduced AGS gastric cancer cell invasion and metastasis by enhancing anoikis sensitivity. PMID: 26986949
      47. High serum alpha-fetoprotein is associated with colon cancer. PMID: 26976278
      48. High serum AFP is associated with hepatitis B virus infection-related hepatocellular carcinoma. PMID: 26784252
      49. Elevated insulin resistance, leptin, serum iron, ferritin, mean platelet volume, NLR, and advanced fibrosis, as well as decreased platelet count and serum albumin, are independently associated with an elevated AFP level in chronic hepatitis C. PMID: 26618566
      50. VEGF could be a potential biomarker for HCC, while AFP could be used to distinguish between patients with HCC and cirrhosis or HCV. PMID: 26782388



    • 相关疾病:
      Alpha-fetoprotein deficiency (AFPD); Alpha-fetoprotein, hereditary persistence (HPAFP)
    • 亚细胞定位:
    • 蛋白家族:
      ALB/AFP/VDB family
    • 组织特异性:
      Plasma. Synthesized by the fetal liver and yolk sac.
    • 数据库链接:

      HGNC: 317

      OMIM: 104150

      KEGG: hsa:174

      STRING: 9606.ENSP00000379138

      UniGene: Hs.518808

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