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    Human β-actin ELISA Kit

    • 中文名称:
      人β-肌动蛋白 ELISA Kit
    • 货号:
      CSB-E13298h
    • 规格:
      96T/48T
    • 价格:
      ¥3200/¥2500
    • 其他:

    产品详情

    • 产品描述:

      This Human ACTB ELISA Kit was designed for the quantitative measurement of Human ACTB protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

    • 别名:
      A26C1A ELISA Kit; A26C1B ELISA Kit; ACTB ELISA Kit; ACTB_HUMAN ELISA Kit; Actin beta ELISA Kit; Actin cytoplasmic 1 ELISA Kit; Actin; cytoplasmic 1; N-terminally processed ELISA Kit; Actx ELISA Kit; b actin ELISA Kit; b-actin ELISA Kit; Beta cytoskeletal actin ELISA Kit; Beta-actin ELISA Kit; BRWS1 ELISA Kit; E430023M04Rik ELISA Kit; Melanoma X actin ELISA Kit; MGC128179 ELISA Kit; PS1TP5 binding protein 1 ELISA Kit; PS1TP5BP1 ELISA Kit
    • 缩写:
      ACTB
    • Uniprot No.:
    • 种属:
      Homo sapiens (Human)
    • 样本类型:
      serum, plasma, tissue homogenates, cell lysates
    • 检测范围:
      0.312 ng/mL-20 ng/mL
    • 灵敏度:
      0.078 ng/mL
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Signal Transduction
    • 测定原理:
      quantitative
    • 测定方法:
      Sandwich
    • 说明书:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%
      Three samples of known concentration were tested twenty times on one plate to assess.
      Inter-assay Precision (Precision between assays): CV%<10%
      Three samples of known concentration were tested in twenty assays to assess.
    • 线性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of human β-actin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
       SampleSerum(n=4)
      1:1Average %94
      Range %80-105
      1:2Average %92
      Range %85-100
      1:4Average %97
      Range %92-105
      1:8Average %90
      Range %83-100
    • 回收率:
      The recovery of human β-actin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample TypeAverage % RecoveryRange
      Serum (n=5) 9582-100
      EDTA plasma (n=4)9589-105
    • 标准曲线:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      ng/mlOD1OD2AverageCorrected
      202.652 2.641 2.647 2.497
      102.134 1.993 2.064 1.914
      51.548 1.486 1.517 1.367
      2.50.987 0.960 0.974 0.824
      1.250.595 0.578 0.587 0.437
      0.6250.402 0.397 0.400 0.250
      0.3120.263 0.245 0.254 0.104
      00.154 0.145 0.150  
    • 数据处理:
    • 货期:
      3-5 working days

    产品评价

    靶点详情

    • 功能:
      Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.
    • 基因功能参考文献:
      1. During cell adhesion, an active multistage process naturally leads to pattern transitions from actin vortices over stars into asters. PMID: 28194011
      2. Haploinsufficiency of ACTB is the main cause of the clinical phenotype observed in the patients with 7p22.1 microdeletions. PMID: 29274487
      3. Baraitser-Winter cerebrofrontofacial syndrome is caused by missense mutations in the cytoplasmic beta- and gamma-actin genes ACTB and ACTG1. We provide an overview of the clinical characteristics (including some novel findings in four recently diagnosed patients), diagnosis, management, mutation spectrum and genetic counselling. PMID: 27625340
      4. The authors describe heterozygous ACTB deletions and nonsense and frameshift mutations in 33 individuals with developmental delay, apparent intellectual disability, increased frequency of internal organ malformations (including those of the heart and the renal tract), growth retardation, and a recognizable facial gestalt. PMID: 29220674
      5. Data indicate AIM1 (absent in melanoma 1) as an actin binding protein and show that it regulates cytoskeletal remodeling and cell migration in prostate epithelial cells. PMID: 28747635
      6. Case Report: gastric pericytoma harboring the exceptionally rare translocation t(7;12) resulting in ACTB-GLI1 gene fusion. PMID: 26980027
      7. Data suggest that, in T-lymphocytes, nitric oxide generated by eNOS S-nitrosylates Cys374 on ACTB and thus regulates activation/recruitment of PRKCQ at immune synapse; S-nitrosylation of beta-actin impairs actin binding to PFN1 and regulates protein transport in lamellipodia. (eNOS = nitric oxide synthase 3; ACTB = beta-actin; PRKCQ = protein kinase C-theta; PFN1 = profilin-1) PMID: 28394935
      8. Data indicate that the IQGAP1 N-terminal fragment spanning residues 1-191 (CHDF) binds to both F-actin and Ca(2+)/calmodulin. PMID: 27798963
      9. Based on present and published dup7p22.1 patients we suggest that renal abnormalities might be an additional feature of the 7p22.1 microduplication syndrome.We also pinpoint the ACTB gene as the key gene affecting the 7p22.1 duplication syndrome phenotype. PMID: 27866048
      10. We suggest that haploinsufficiency of ACTB may be responsible for the clinical features of patients with 7p22.1 microdeletions PMID: 27633570
      11. crucial role of Glu270 in ADP-ribosylation of actin by Ia PMID: 26713879
      12. Studies indicate that the process of megakaryocyte maturation and the formation of proplatelets are essential steps in the production of mature platelets and both depend heavily on the actin and microtubule cytoskeletons. PMID: 26210823
      13. Data show that tripartite motif-containing 28 protein (TRIM28) and beta-actin were up-regulated in the glioblastoma multiforme (GBM) stem-like cells compared to the controls. PMID: 25419715
      14. Data suggest that by binding to both clathrin and F-actin, mammalian actin-binding protein 1 (mAbp1; HIP-55 or SH3P7) is specifically recruited at a late stage of clathrin-coated pits (CCPs) formation, which subsequently recruits dynamin to CCPs. PMID: 25690657
      15. The results indicate that the disease-related human beta-actin mutations p.R183W and p.E364K affect interdomain mobility and nucleotide interactions as a basis for the formation of disease phenotypes in patients. PMID: 25255767
      16. Data indicate the WASp-interacting protein (WIP)-Wiskott-Aldrich syndrome protein (WASp) interaction in the regulation of actin-dependent processes. PMID: 24962707
      17. Mutations in ACTB cause a distinctly more severe phenotype than ACTG1 mutations in Baraitser-Winter syndrome. PMID: 23756437
      18. TIA proteins can function as long-term regulators of the ACTB mRNA metabolism in mouse and human cells. PMID: 24766723
      19. Downregulation of the HuR gene results in decreased beta-actin gene expression, which in turn results in decreased motility and proliferation of corneal fibroblasts. PMID: 24826067
      20. Thus, the nucleocapsid domain in HIV-1 Gag does not appear to have a role in actin recruitment or actin incorporation into HIV-1 particles. PMID: 24789788
      21. Taken together, these findings indicate that actin filament dynamics are dispensable for HIV-1 Gag assembly at the plasma membrane of HeLa cells. PMID: 24789789
      22. Results indicate that actin cytoskeleton is one of the upstream regulators of Hippo signaling. PMID: 24040060
      23. PDI appears to regulate cytoskeletal reorganization by the thiol-disulfide exchange in beta-actin via a redox-dependent mechanism. PMID: 24415753
      24. chorein interacts with beta-adducin and beta-actin. PMID: 24129186
      25. Data indicate that monomeric actin probes concentrate in nuclear speckles. PMID: 23447706
      26. the roles of ACTB in tumors PMID: 23266771
      27. Data suggest that P-glycoprotein associate with the F-actin cytoskeleton through ezrin/radixin/moesin (ERM) in CCR9/CCL25 induced multidrug resistance of acute T-lymphocytic leukemia (T-ALL) cells. PMID: 23326330
      28. Two variants of beta-actin, beta1 and beta2 were found in the Enterovirus 71-susceptible rhabdomyosarcoma cells, compared to Enterovirus 71-resistant cells that contains only one variant beta1. PMID: 23535377
      29. Studies indicate that cofilin binds actin stoichiometrically - one cofilin molecule per actin filament subunit. PMID: 23395798
      30. Studies indicate that that vinculin not only bundles actin filaments but can also cap these filaments and promote actin polymerization. PMID: 23466368
      31. Cofilin nuclear shuttling is critical for the cofilin-actin rod stress response. PMID: 22623727
      32. These results indicate that F-actin in association with the M protein alters the interaction between the M and H proteins, thereby modulating measles virus cell-cell fusion and assembly. PMID: 23221571
      33. Data indicate beta-cytoplasmic (beta-CYA) and gamma-cytoplasmic (gamma-CYA) actins differential localization and dynamics at epithelial junctions. PMID: 22855531
      34. roles for undetected ACtB in liver cancer progression PMID: 22961449
      35. Data show that ENOA, PARK7 and Beta-actin are proper reference standards in obesity studies based on omental fat. PMID: 22272336
      36. identified de novo missense changes in the cytoplasmic actin-encoding genes ACTB and ACTG1 in one and two probands, respectively; suggest that Baraitser-Winter syndrome is the predominant phenotype associated with mutation of these two genes PMID: 22366783
      37. Recombinant human actin is constantly shuttled into the murine nucleus by importin 9 and out by exportin 6. Nuclear actin is required for maximal transcription. PMID: 22323606
      38. Antioxidant supplementation was noted to increase G6PDH in the pentose phosphate cycle and 18S rRNA in the ribosome. There were no significant changes in the gene expression levels of beta-ACT PMID: 22285204
      39. non-muscle alpha-ACTN4, HSPA5 and cytoplasmic ACTB, should be targeted in idiopathic premature ovarian failure cases PMID: 21890413
      40. Data indicate taht candidate genes ACTB, BZW, OCM, MACC1, NXPH1, PRPS1L1, RAC1 and RPA3, which lie within the DFNB90 region, were sequenced and no potentially causal variants were identified. PMID: 21734401
      41. ACTB showed high expression in forensic skin and body-fluid samples, providing a suitable marker for skin identification PMID: 21221983
      42. Nuclear beta-actin controls growth arrest of epithelial cells. PMID: 21172822
      43. Data suggest that the existence of a common epitope on the molecules of phosducin and beta-actin may reflect a topological similarity of a small region of their surfaces. PMID: 20804785
      44. Findings indicate that activation of the cofilin-F-actin pathway contributes to tumor cell migration and metastasis enhanced by Aur-A, revealing a novel function for mitotic Aur-A kinase in tumor progression. PMID: 21045147
      45. The actin network plays a role in nuclear ERalpha actions in breast cancer cells. PMID: 20308691
      46. Immunoblot analysis revealed profoundly decreased beta-actin levels during Ectromelia virus infection replicative cycle in the infected cells 24 hrs post infection. PMID: 20201613
      47. This protein has been found differentially expressed in the anterior cingulate cortex from patients with schizophrenia PMID: 20381070
      48. Data show that inducible Hox genes are selectively sensitive to the inhibition of actin polymerization and that actin polymerization is required for the assembly of a transcription complex on the regulatory region of the Hox genes. PMID: 19477923
      49. Results suggest a novel mechanism by which cofilin is regulated by v-Src through tyrosine phosphorylation that triggers the degradation of cofilin through ubiquitination-proteosome pathway and reducing cellular F-actin contents and cell spreading. PMID: 19802004
      50. region responsible for down-regulation of the gamma-actin gene during differentiation is not in the 3' end of the gene in contrast to that for beta-actin. PMID: 11787062

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    • 相关疾病:
      Dystonia, juvenile-onset (DJO); Baraitser-Winter syndrome 1 (BRWS1)
    • 亚细胞定位:
      Cytoplasm, cytoskeleton. Nucleus.
    • 蛋白家族:
      Actin family
    • 数据库链接:

      HGNC: 132

      OMIM: 102630

      KEGG: hsa:60

      STRING: 9606.ENSP00000349960

      UniGene: Hs.520640



    1v1周青梅郑宣木天寥
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