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  • Your Good Partner in Biology Research

    Goat Cortisol ELISA Kit

    • 中文名称:
      山羊皮质醇(Cortisol)ELISA Kit
    • 货号:
      CSB-E18048G
    • 规格:
      96T/48T
    • 价格:
      ¥4200/¥3000
    • 其他:

    产品详情

    • 产品描述:

      This Goat COR ELISA Kit was designed for the quantitative measurement of Goat COR protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.05 ng/mL-200 ng/mL and the sensitivity is 0.05 ng/mL.

    • 缩写:
      COR
    • 种属:
      Capra hircus (Goat)
    • 样本类型:
      serum, plasma, tissue homogenates
    • 检测范围:
      0.05 ng/mL-200 ng/mL
    • 灵敏度:
      0.05 ng/mL
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Metabolism
    • 测定原理:
      quantitative
    • 测定方法:
      Competitive
    • 说明书:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<10%      
      Three samples of known concentration were tested in twenty assays to assess.    
                   
    • 线性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of goat cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
        Sample Serum(n=4)  
      1:1 Average % 93  
      Range % 86-97  
      1:2 Average % 97  
      Range % 92-102  
      1:4 Average % 88  
      Range % 85-90  
      1:8 Average % 90  
      Range % 84-95  
    • 回收率:
      The recovery of goat cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 90 88-92  
      EDTA plasma (n=4) 94 90-99  
                   
                   
    • 标准曲线:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      ng/ml OD1 OD2 Average    
      200 0.178 0.190 0.184    
      50 0.256 0.267 0.262    
      12.5 0.442 0.473 0.458    
      3.12 0.674 0.672 0.673    
      0.78 1.043 1.024 1.034    
      0.195 1.321 1.310 1.316    
      0.05 1.688 1.707 1.698    
      0 2.049 1.924 1.987    
    • 数据处理:
    • 货期:
      3-5 working days

    产品评价

    问答及客户评论

     常见问题解答
    Q:

    I'm a PhD Candidate studying metabolic stress in dairy goats in the peripartum.
    In total, 450 samples have to be analysed for each hormone, and analysis will be done in duplicate. Meaning I'll need a number of kits enough to run 900 tests.
    Could you please send me a quote specifying the plate size (i.e. 96 well) and the number of free wells per plate. (i.e. 96 well minus the number of wells used for the standard curve).

    A:
    Thanks for your email!Regarding your request,we recommand this kit to you.
    #CSB-E18048G,Goat Cortisol ELISA Kit *96T (If duplicate done,24 kits is advised;If not,only 12 kits is ok)
    If you want to detect diary goat samples,we suggest you do pre-testing first.
    Q:

    I have a customer who is looking for Goat Cortisol (COR) ELISA Kit to test with feces samples. Please confirm that the kit is suitable for feces samples.
    Per customer request, can you please provide the following information about this kit:
    a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
    b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
    c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
    d. Please provide your reference data for feces samples. If your lab didn’t test feces, please provide available reference data.
    f. Please send the current manual.

    A:
    Thanks for your inquiry!
    CSB-E18048G
    This kit detects small molecule and it is based on competitive method.
    The plate is coated with standard conjugate. The polyclonal antibody used in the kit is rabbit polyclonal antibody. The immunogen is modified standard. The secondary antibody used in the kit is goat-anti-rabbit antibody .The immunogen is rabbit IgG. Standard is purified small molecule.
    We tested the mentioned sample stated in the manual..We suggest you follow the manual. We tested serum and plasma samples under 1:20 dilution before and the test value is 3-12.5ng/ml. The final concentration is 60--250ng/ml. You can refer to it.We can not ensure 100% on feces sample.If you want to test feces sample we suggest you do a pretest first if you want to test it.
    And I will send you manual by email later.
     客户评论
    • Sample type: Plasma
      Sample species: Goat
      Review: To investigated the plasma cortisol level of the goat during the summer and winter season.


    1v1周青梅郑宣木天寥
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