Fish dopamine(DA) ELISA kit
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中文名称:鱼多巴胺(DA)ELISA kit
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货号:CSB-EQ027496FI
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规格:96T/48T
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价格:¥4200/¥3000
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其他:
产品详情
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产品描述:
This Fish dopamine (DA) ELISA Kit was designed for the quantitative measurement of Fish dopamine (DA) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 62.5 pg/mL-1000 pg/mL and the sensitivity is 62.5 pg/mL.
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缩写:DA
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种属:Fish
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样本类型:serum, plasma, tissue homogenates
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检测范围:62.5 pg/mL-1000 pg/mL
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灵敏度:62.5 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Neuroscience
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测定原理:quantitative
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测定方法:Competitive
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说明书:
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of fish DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 92-104 1:2 Average % 94 Range % 91-98 1:4 Average % 102 Range % 98-106 1:8 Average % 88 Range % 82-94 -
回收率:
The recovery of fish DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-98 EDTA plasma (n=4) 103 98-107 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 1000 0.154 0.155 0.155 500 0.278 0.272 0.275 250 0.503 0.518 0.511 125 1.135 1.174 1.155 62.5 2.345 2.428 2.387 -
数据处理:
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货期:3-5 working days
引用文献
- Different action mechanisms of low-and high-level quercetin in the brains of adult zebrafish (Danio rerio) X Wu,Ecotoxicology and Environmental Safety,2021
- Assessment of the Effects of Bisphenol A on Dopamine Synthesis and Blood Vessels in the Goldfish Brain Wang Q, et al,International Journal of Molecular Sciences,2019
- Venlafaxine plus melatonin ameliorate reserpine-induced depression-like behavior in zebrafish Ya-Qiu Tang, et al,Neurotoxicology and Teratology,2019
- Transcriptional analysis of renal dopamine-mediated Na+ homeostasis response to environmental salinity stress in Scatophagus argus Su M,BMC Genomics,2019
- Dopamine regulates renal osmoregulation during hyposaline stress via DRD1 in the spotted scat (Scatophagus argus) Maoliang Su.et al,Scientific Reports,2016
- Changes of physiological rhythms of N-methyl-d-aspartate receptors in the chum salmon Oncorhynchus keta: effect of seawater acclimation during the parr-smolt transformation Young Jae Choi.et al,Biological Rhythm Research,2016
- Ambient particulate air pollution and circulating antioxidant enzymes: A repeated-measure study in healthy adults in Beijing, China Shaowei Wu. et al,Environ Pollut,2015
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