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  • Your Good Partner in Biology Research

    Fish Cortisol ELISA Kit

    • 中文名称:
      鱼类皮质醇(Cortisol)ELISA Kit
    • 货号:
      CSB-E08487f
    • 规格:
      96T/48T
    • 价格:
      ¥4200/¥3000
    • 其他:

    产品详情

    • 产品描述:

      This Fish COR ELISA Kit was designed for the quantitative measurement of Fish COR protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.0023 ng/mL-10 ng/mL and the sensitivity is 0.0023 ng/mL .

    • 缩写:
      COR
    • 种属:
      Fish
    • 样本类型:
      serum, plasma, tissue homogenates
    • 检测范围:
      0.0023 ng/mL-10 ng/mL
    • 灵敏度:
      0.0023 ng/mL
    • 反应时间:
      1-5h
    • 样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 研究领域:
      Metabolism
    • 测定原理:
      quantitative
    • 测定方法:
      Competitive
    • 说明书:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<10%      
      Three samples of known concentration were tested in twenty assays to assess.    
                   
    • 线性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of fish Cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
        Sample Serum(n=4)  
      1:20 Average % 90  
      Range % 86-98  
      1:40 Average % 96  
      Range % 92-100  
      1:80 Average % 82  
      Range % 81-88  
      1:160 Average % 95  
      Range % 91-101  
    • 回收率:
      The recovery of fish Cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 91 87-96  
      EDTA plasma (n=4) 98 92-105  
                   
                   
    • 标准曲线:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      ng/ml OD1 OD2 Average    
      10 0.207 0.196 0.202    
      2.5 0.396 0.381 0.389    
      0.625 0.531 0.575 0.553    
      0.156 0.948 0.982 0.965    
      0.039 1.326 1.390 1.358    
      0.01 1.855 1.996 1.926    
      0.0023 2.097 2.200 2.149    
      0 2.367 2.302 2.335    
    • 数据处理:
    • 货期:
      3-5 working days

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    1v1周青梅郑宣木天寥
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