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  • Your Good Partner in Biology Research

    Chicken Interleukin 6,IL-6 ELISA Kit

    • 中文名稱:
      雞白介素6(IL-6)ELISA Kit
    • 貨號:
      CSB-E08549Ch
    • 規格:
      96T/48T
    • 價格:
      ¥3800/¥2500
    • 其他:

    產品詳情

    • 產品描述:

      This Chicken IL6 ELISA Kit was designed for the quantitative measurement of Chicken IL6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

    • 別名:
      IL6 ELISA Kit; Interleukin-6 ELISA Kit; ChIL-6 ELISA Kit; IL-6 ELISA Kit
    • 縮寫:
      IL6
    • Uniprot No.:
    • 種屬:
      Gallus gallus (Chicken)
    • 樣本類型:
      serum, plasma, tissue homogenates
    • 檢測范圍:
      15.6 pg/mL-1000 pg/mL
    • 靈敏度:
      3.9 pg/mL
    • 反應時間:
      1-5h
    • 樣本體積:
      50-100ul
    • 檢測波長:
      450 nm
    • 研究領域:
      Immunology
    • 測定原理:
      quantitative
    • 測定方法:
      Sandwich
    • 說明書:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%
      Three samples of known concentration were tested twenty times on one plate to assess.
      Inter-assay Precision (Precision between assays): CV%<10%
      Three samples of known concentration were tested in twenty assays to assess.
    • 線性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
       SampleSerum(n=4)
      1:1Average %93
      Range %85-97
      1:2Average %92
      Range %85-99
      1:4Average %95
      Range %89-100
      1:8Average %92
      Range %85-100
    • 回收率:
      The recovery of chicken IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample TypeAverage % RecoveryRange
      Serum (n=5) 9891-105
      EDTA plasma (n=4)9688-99
    • 標準曲線:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      pg/mlOD1OD2AverageCorrected
      10002.487 2.570 2.529 2.454
      5002.204 2.230 2.217 2.142
      2501.800 1.764 1.782 1.707
      1251.390 1.394 1.392 1.317
      62.50.917 0.950 0.934 0.859
      31.20.584 0.568 0.576 0.501
      15.60.348 0.362 0.355 0.280
      00.074 0.075 0.075  
    • 數據處理:
    • 貨期:
      3-5 working days

    引用文獻

    產品評價

    靶點詳情

    • 功能:
      Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
    • 基因功能參考文獻:
      1. Avian leukosis virus subgroup J induces VEGF expression via NF-kappaB/PI3K-dependent IL-6 production to promoter tumorigenesis. PMID: 27852059
      2. The effects of daily corticosterone injections on acute phase proteins (APP), IL-6, and HSP70 are reported; APP and HSP70 may be of value as indicators of stress in poultry. PMID: 25306460
      3. Chicken IL-6 is a heat-shock gene PMID: 24055475
      4. Nampt/visfatin/PBEF affects expression of myogenic regulatory factors and is regulated by interleukin-6 in chicken skeletal muscle cells PMID: 21545843
      5. IL-6 is involved in the progression of chicken arthritis induced by Staph. aureus, and it contributes to disease incidence and mortality PMID: 17495100

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    • 亞細胞定位:
      Secreted.
    • 蛋白家族:
      IL-6 superfamily
    • 數據庫鏈接:

      KEGG: gga:395337

      UniGene: Gga.2769



    1v1周青梅郑宣木天寥
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