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  • Your Good Partner in Biology Research

    Chicken Interleukin 10,IL-10 ELISA Kit

    • 中文名稱:
      雞白介素10(IL-10)ELISA Kit
    • 貨號:
      CSB-E12835C
    • 規格:
      96T/48T
    • 價格:
      ¥3800/¥2500
    • 其他:

    產品詳情

    • 產品描述:

      This Chicken IL10 ELISA Kit was designed for the quantitative measurement of Chicken IL10 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1 pg/mL-200 pg/mL and the sensitivity is 0.5 pg/mL.

    • 別名:
      IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit
    • 縮寫:
      IL10
    • Uniprot No.:
    • 種屬:
      Gallus gallus (Chicken)
    • 樣本類型:
      serum, plasma, tissue homogenates
    • 檢測范圍:
      1 pg/mL-200 pg/mL
    • 靈敏度:
      0.5 pg/mL
    • 反應時間:
      1-5h
    • 樣本體積:
      50-100ul
    • 檢測波長:
      450 nm
    • 研究領域:
      Immunology
    • 測定原理:
      quantitative
    • 測定方法:
      Competitive
    • 說明書:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<15%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<15%      
      Three samples of known concentration were tested in twenty assays to assess.    
                   
    • 線性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
        Sample Serum(n=4)  
      1:1 Average % 96  
      Range % 90-101  
      1:2 Average % 97  
      Range % 94-105  
      1:4 Average % 95  
      Range % 89-100  
      1:8 Average % 92  
      Range % 87-96  
    • 回收率:
      The recovery of chicken IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 94 90-98  
      EDTA plasma (n=4) 97 93-101  
                   
                   
    • 標準曲線:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      pg/ml OD1 OD2 Average    
      200 0.155 0.152 0.154    
      50 0.334 0.348 0.341    
      16 0.515 0.502 0.509    
      4 0.969 0.975 0.972    
      1 1.436 1.463 1.450    
      0 2.658 2.667 2.663    
    • 數據處理:
    • 貨期:
      3-5 working days

    引用文獻

    產品評價

    靶點詳情

    • 功能:
      Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
    • 亞細胞定位:
      Secreted.
    • 蛋白家族:
      IL-10 family
    • 組織特異性:
      Expressed predominantly in bursa of Fabricius and cecal tonsils with low levels in thymus, liver and lung.
    • 數據庫鏈接:


    1v1周青梅郑宣木天寥
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