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  • Your Good Partner in Biology Research

    Chicken Interferon γ ,IFN-γ ELISA Kit

    • 中文名稱:
      雞γ干擾素(IFN-γ)ELISA Kit
    • 貨號:
      CSB-E08550Ch
    • 規格:
      96T/48T
    • 價格:
      ¥4200/¥3000
    • 其他:

    產品詳情

    • 產品描述:

      This Chicken IFNG ELISA Kit was designed for the quantitative measurement of Chicken IFNG protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.125 pg/mL-800 pg/mL and the sensitivity is 3.125 pg/mL.

    • 別名:
      IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
    • 縮寫:
      IFNG
    • Uniprot No.:
    • 種屬:
      Gallus gallus (Chicken)
    • 樣本類型:
      serum, plasma, tissue homogenates
    • 檢測范圍:
      3.125 pg/mL-800 pg/mL
    • 靈敏度:
      3.125 pg/mL
    • 反應時間:
      1-5h
    • 樣本體積:
      50-100ul
    • 檢測波長:
      450 nm
    • 研究領域:
      Immunology
    • 測定原理:
      quantitative
    • 測定方法:
      Competitive
    • 說明書:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<10%      
      Three samples of known concentration were tested in twenty assays to assess.    
                   
    • 線性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
        Sample Serum(n=4)  
      1:1 Average % 96  
      Range % 91-100  
      1:2 Average % 92  
      Range % 88-97  
      1:4 Average % 102  
      Range % 98-106  
      1:8 Average % 94  
      Range % 90-98  
    • 回收率:
      The recovery of chicken IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 97 93-101  
      EDTA plasma (n=4) 92 88-96  
                   
                   
    • 標準曲線:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      pg/ml OD1 OD2 Average    
      800 0.178 0.182 0.180    
      200 0.245 0.233 0.239    
      50 0.457 0.482 0.470    
      12.5 0.848 0.817 0.833    
      3.125 1.396 1.364 1.380    
      0 2.237 2.242 2.240    
    • 數據處理:
    • 貨期:
      3-5 working days

    引用文獻

    產品評價

    靶點詳情

    • 功能:
      Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
    • 基因功能參考文獻:
      1. Knockdown of IFN-gamma did not seem to fully abrogate vaccine-induced protection against Marek's disease in chickens. PMID: 25673902
      2. Vitiligo in Smyth line chickens appears to be a Th1-polarized autoimmune disease, whereby IFN-gamma expression is strongly associated with parallel increases in IL-10 and IL-21, particularly during the early and active stages of vitiligo. PMID: 22113479
      3. The dose-response effects of supplemental dietary chormium on interferon-gamma expression in response to Newcastle disease virus vaccine in broiler chickens is reported. PMID: 17888472
      4. The pivotal role of chicken interferon-gamma in the pathogenesis and immunosuppression of infectious bursal disease virus infection in in vivo, ex vivo and in vitro experiments are reported. PMID: 17899460
      5. that in vitro stimulation with IBV resulted in ChIFN-gamma production in splenocytes of both infected birds and uninfected birds PMID: 19070879

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    • 亞細胞定位:
      Secreted.
    • 蛋白家族:
      Type II (or gamma) interferon family
    • 數據庫鏈接:

      KEGG: gga:396054

      STRING: 9031.ENSGALP00000016086

      UniGene: Gga.916



    1v1周青梅郑宣木天寥
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