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  • Your Good Partner in Biology Research

    Bovine Cortisol ELISA Kit

    • 中文名稱:
      牛皮質醇(Cortisol)ELISA Kit
    • 貨號:
      CSB-E13064B
    • 規格:
      96T/48T
    • 價格:
      ¥4200/¥3000
    • 其他:

    產品詳情

    • 產品描述:

      This Bovine COR ELISA Kit was designed for the quantitative measurement of Bovine COR protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.049 ng/mL-200 ng/mL and the sensitivity is 0.049 ng/mL.

    • 縮寫:
      COR
    • 種屬:
      Bos taurus (Bovine)
    • 樣本類型:
      serum, plasma, tissue homogenates
    • 檢測范圍:
      0.049 ng/mL-200 ng/mL
    • 靈敏度:
      0.049 ng/mL
    • 反應時間:
      1-5h
    • 樣本體積:
      50-100ul
    • 檢測波長:
      450 nm
    • 研究領域:
      Metabolism
    • 測定原理:
      quantitative
    • 測定方法:
      Competitive
    • 說明書:
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<10%      
      Three samples of known concentration were tested in twenty assays to assess.    
                   
    • 線性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of bovine cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
        Sample Serum(n=4)  
      1:1 Average % 92  
      Range % 86-99  
      1:2 Average % 96  
      Range % 92-105  
      1:4 Average % 85  
      Range % 81-89  
      1:8 Average % 91  
      Range % 83-98  
    • 回收率:
      The recovery of bovine cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 91 87-95  
      EDTA plasma (n=4) 96 92-102  
                   
                   
    • 標準曲線:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      ng/ml OD1 OD2 Average    
      200 0.194 0.184 0.189    
      50 0.328 0.320 0.324    
      12.5 0.507 0.550 0.529    
      3.12 0.900 0.975 0.938    
      0.78 1.314 1.266 1.290    
      0.195 1.653 1.657 1.655    
      0.049 1.915 1.902 1.909    
      0 2.306 2.400 2.353    
    • 數據處理:
    • 貨期:
      3-5 working days

    引用文獻

    產品評價

    問答及客戶評論

     常見問題解答
    Q:

    Please provide the following information regarding Bovine Cortisol ELISA Kit (CSB-E13064B):
    1. What is the Uniprot/Accession number that this kit is designed for? Is the kit designed for XYZ species or is it designed for a different species such as Human and cross reacts with XYZ species?
    2. Is the capture antibody a monoclonal or polyclonal?
    3. What's is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
    4. If recombinant protein, is it full length or partial?
    5. What is the species of the standard– human or something else?
    6. What sample type was tested with this kit (serum? plasma? Something else?) and what were the reference values.

    A:
    Thanks for your inquiry.
    Cortisol is small molecule and it is the same in all species, so there is no uniprot.
    Since for different species, the matrix is different, we have developed kits for different species.
    CSB-E13064B
    The standard is purified cortisol. This is a competitive kit. The plate is coated with specific antigen. The specific antibody is rabbit polyclonal antibody. The immunogen is modified by special modification.
    HRP is conjugated with secondary antibody( goat anti-rabbit antibody).
    Pls let me know if you have any further questions. Thank you.


    1v1周青梅郑宣木天寥
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